AB30687

Anti-LAMP1 antibody - Lysosome Marker

Name: Anti-LAMP1 antibody - Lysosome Marker (ab30687) | Abcam
Brand: Abcam Limited
SKU: AB30687
Rating: 4 (4 reviews)

Lab Essentials
What is this?

(4 Reviews)

(32 Publications)

Rabbit Polyclonal LAMP1 antibody. Lysosome marker. Suitable for ICC and reacts with Drosophila melanogaster samples. Cited in 32 publications.

1 Images

Supplier Data

Immunocytochemistry - Anti-LAMP1 antibody - Lysosome Marker (AB30687)

ab30687 staining LAMP1 in Drosophila S2 Schneider cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab30687 at 0.2μg/ml concentration and ab44928 (Mouse monoclonal to Tubulin) at 0.2μg/ml concentration overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Drosophila melanogaster

Applications

ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Drosophila melanogaster": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.2 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information LAMP1

style

left-column

Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:328 PubMed40263294

2025

Opposing roles for GSK3β and ERK1-dependent phosphorylation of huntingtin during neuronal dysfunction and cell death in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Krzystek,Rasika Rathnayake,Jia Zeng,Jing Huang,Gary Iacobucci,Michael C Yu,Shermali Gunawardena

Autophagy 20:131-150 PubMed37649246

2023

Atl (atlastin) regulates mTor signaling and autophagy in Drosophila muscle through alteration of the lysosomal network.

Applications

Unspecified application

Species

Unspecified reactive species

Saurabh Srivastav,Kevin van der Graaf,Pratibha Singh,Alloysius Budi Utama,Matthew D Meyer,James A McNew,Michael Stern

iScience 26:107335 PubMed37529104

2023

Role of Rab5 early endosomes in regulating gut antibacterial response.

Applications

Unspecified application

Species

Unspecified reactive species

Manish Joshi,Annelise Viallat-Lieutaud,Julien Royet

Nature 617:798-806 PubMed37138087

2023

A phosphate-sensing organelle regulates phosphate and tissue homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chiwei Xu,Jun Xu,Hong-Wen Tang,Maria Ericsson,Jui-Hsia Weng,Jonathan DiRusso,Yanhui Hu,Wenzhe Ma,John M Asara,Norbert Perrimon

Development (Cambridge, England) 150: PubMed36861793

2023

The Drosophila AWP1 ortholog Doctor No regulates JAK/STAT signaling for left-right asymmetry in the gut by promoting receptor endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ting Lai,Takeshi Sasamura,Junpei Kuroda,Reo Maeda,Mitsutoshi Nakamura,Ryo Hatori,Tomoki Ishibashi,Kiichiro Taniguchi,Masashi Ooike,Tomohiro Taguchi,Naotaka Nakazawa,Shunya Hozumi,Takashi Okumura,Toshiro Aigaki,Mikiko Inaki,Kenji Matsuno

Aging cell 21:e13715 PubMed36259256

2022

Autophagy activation can partially rescue proteasome dysfunction-mediated cardiac toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Eleni-Dimitra Papanagnou,Sentiljana Gumeni,Aimilia D Sklirou,Alexandra Rafeletou,Evangelos Terpos,Kleoniki Keklikoglou,Efstathios Kastritis,Kimon Stamatelopoulos,Gerasimos P Sykiotis,Meletios A Dimopoulos,Ioannis P Trougakos

Journal of extracellular vesicles 11:e12263 PubMed36103151

2022

Microscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo model.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Linnemannstöns,Pradhipa Karuna M,Leonie Witte,Dolma Choezom,Mona Honemann-Capito,Alex Simon Lagurin,Chantal Vanessa Schmidt,Shreya Shrikhande,Lara-Kristin Steinmetz,Möbius Wiebke,Christof Lenz,Julia Christina Gross

Autophagy 19:1199-1220 PubMed36048753

2022

HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Krzystek,Joseph A White,Rasika Rathnayake,Layne Thurston,Hayley Hoffmar-Glennon,Yichen Li,Shermali Gunawardena

JCI insight 7: PubMed35801587

2022

Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xianzun Tao,Yi Zhu,Zoraida Diaz-Perez,Seok-Ho Yu,Jackson R Foley,Tracy Murray Stewart,Robert A Casero,Richard Steet,R Grace Zhai

eLife 10: PubMed33667157

2021

sustains trans-synaptic BMP signaling required for synaptic maintenance with age.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica M Sidisky,Daniel Weaver,Sarrah Hussain,Meryem Okumus,Russell Caratenuto,Daniel Babcock

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMP1 antibody - Lysosome Marker

Immunocytochemistry - Anti-LAMP1 antibody - Lysosome Marker (AB30687)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise