Rabbit Polyclonal LAMP1 antibody. Lysosome marker. Suitable for ICC and reacts with Drosophila melanogaster samples. Cited in 27 publications.
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- Nature 617:798-8062023A phosphate-sensing organelle regulates phosphate and tissue homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChiwei Xu,Jun Xu,Hong-Wen Tang,Maria Ericsson,Jui-Hsia Weng,Jonathan DiRusso,Yanhui Hu,Wenzhe Ma,John M Asara,Norbert PerrimonPubMed 37138087
- Development (Cambridge, England) 150:2023The Drosophila AWP1 ortholog Doctor No regulates JAK/STAT signaling for left-right asymmetry in the gut by promoting receptor endocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Ting Lai,Takeshi Sasamura,Junpei Kuroda,Reo Maeda,Mitsutoshi Nakamura,Ryo Hatori,Tomoki Ishibashi,Kiichiro Taniguchi,Masashi Ooike,Tomohiro Taguchi,Naotaka Nakazawa,Shunya Hozumi,Takashi Okumura,Toshiro Aigaki,Mikiko Inaki,Kenji MatsunoPubMed 36861793
- Aging cell 21:e137152022Autophagy activation can partially rescue proteasome dysfunction-mediated cardiac toxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEleni-Dimitra Papanagnou,Sentiljana Gumeni,Aimilia D Sklirou,Alexandra Rafeletou,Evangelos Terpos,Kleoniki Keklikoglou,Efstathios Kastritis,Kimon Stamatelopoulos,Gerasimos P Sykiotis,Meletios A Dimopoulos,Ioannis P TrougakosPubMed 36259256
- Journal of extracellular vesicles 11:e122632022Microscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaren Linnemannstöns,Pradhipa Karuna M,Leonie Witte,Dolma Choezom,Mona Honemann-Capito,Alex Simon Lagurin,Chantal Vanessa Schmidt,Shreya Shrikhande,Lara-Kristin Steinmetz,Möbius Wiebke,Christof Lenz,Julia Christina GrossPubMed 36103151
- Autophagy 19:1199-12202022HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas J Krzystek,Joseph A White,Rasika Rathnayake,Layne Thurston,Hayley Hoffmar-Glennon,Yichen Li,Shermali GunawardenaPubMed 36048753
- JCI insight 7:2022Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianzun Tao,Yi Zhu,Zoraida Diaz-Perez,Seok-Ho Yu,Jackson R Foley,Tracy Murray Stewart,Robert A Casero,Richard Steet,R Grace ZhaiPubMed 35801587
- eLife 10:2021sustains trans-synaptic BMP signaling required for synaptic maintenance with age.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica M Sidisky,Daniel Weaver,Sarrah Hussain,Meryem Okumus,Russell Caratenuto,Daniel BabcockPubMed 33667157
- Development (Cambridge, England) 147:2020Ykt6-dependent endosomal recycling is required for Wnt secretion in the wing epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaren Linnemannstöns et. al.PubMed 32611603
- Development (Cambridge, England) 147:2020The kinesin motor Klp98A mediates apical to basal Wg transport.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeonie Witte et. al.PubMed 32665246
- The Journal of neuroscience : the official journal 40:6234-62492020Dietary Amino Acids Impact LRRK2-Induced Neurodegeneration in Parkinson's Disease Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVinita G Chittoor-Vinod et. al.PubMed 32605938
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| ICC | WB | |
|---|---|---|
| Drosophila melanogaster | Tested | Not recommended |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info 0.2 µg/mL | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Drosophila melanogaster | Dilution info - | Notes - |
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Target data
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Rabbit Polyclonal LAMP1 antibody. Lysosome marker. Suitable for ICC and reacts with Drosophila melanogaster samples. Cited in 27 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.
- Biological function summary
LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.
- Pathways
The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.
- Associated diseases and disorders
LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunocytochemistry - Anti-LAMP1 antibody - Lysosome Marker (ab30687)
ab30687 staining LAMP1 in Drosophila S2 Schneider cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab30687 at 0.2μg/ml concentration and Anti-Tubulin antibody [DM1A +DM1B] - Loading Control ab44928 (Mouse monoclonal to Tubulin) at 0.2μg/ml concentration overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
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