AB320852

Anti-LAMP1 antibody [RM1217] - BSA and Azide free

BOND RX™ Validated
Recombinant
RabMAb
KO Validated
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Knockout Tested Rabbit Recombinant Multiclonal LAMP1 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), ICC/IF, IP and reacts with Human, Mouse samples.

View Alternative Names

CD107a, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, LAMP1

17 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling LAMP1 with ab320851 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human tonsil. The section was incubated with ab320851 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LAMP1 with ab320851 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human liver. The section was incubated with ab320851 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized LAMP1 KO HAP1 (LAMP1 knockout human chronic myelogenous leukemia near-haploid cell) cells labelling LAMP1 with ab320851 at 1/100 (4.91 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in wildtype HAP1 cells and no staining in LAMP1 knockout HAP1 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling LAMP1 with ab320851 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human lung carcinoma. The section was incubated with ab320851 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded A Wild-type HAP1 (Human chronic myelogenous leukemia near-haploid cell) cell pellet B Human LAMP1 knockout HAP1 cell pellet tissue labeling LAMP1 with ab320851 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on A : Wild-type HAP1 cell pellet, no staining on B : human LAMP1 knockout HAP1 cell pellet. The section was incubated with ab320851 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized LAMP1 KO HAP1 (LAMP1 knock out human chronic myelogenous leukemia near-haploid cell, Right) / Wild-type HAP1 (Left) cells labelling LAMP1 with ab320851 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling LAMP1 with ab320851 at 1/2000 (0.246 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human ovarian cancinoma. The section was incubated with ab320851 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Supplier Data

Immunoprecipitation - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

LAMP1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab320851 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab320851 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : ab320851 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab320851 in HepG2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-LAMP1 antibody [RM1217] - Lysosome Marker (<a href='/en-us/products/primary-antibodies/lamp1-antibody-rm1217-lysosome-marker-ab320851'>ab320851</a>) at 1/30 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling LAMP1 with ab320851 at 1/500 (0.982 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse liver. The section was incubated with ab320851 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling LAMP1 with ab320851 at 1/500 (0.982 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse spleen. The section was incubated with ab320851 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling LAMP1 with ab320851 at 1/100 (4.91 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in NIH/3T3 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling LAMP1 with ab320851 at 1/500 (0.982 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse lung. The section was incubated with ab320851 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling LAMP1 with ab320851 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

LAMP1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab320851 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab320851 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : ab320851 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab320851 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-LAMP1 antibody [RM1217] - Lysosome Marker (<a href='/en-us/products/primary-antibodies/lamp1-antibody-rm1217-lysosome-marker-ab320851'>ab320851</a>) at 1/30 dilution

All lanes:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Supplier Data

Western blot - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab320851 was shown to bind specifically to LAMP1. Target of interest was observed at 110kDa in wild-type HAP1 cell lysates (lane 1) with no signal observed at this size in LAMP1 knockout cell line (lane 2).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 15111122).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-LAMP1 antibody [RM1217] - Lysosome Marker (<a href='/en-us/products/primary-antibodies/lamp1-antibody-rm1217-lysosome-marker-ab320851'>ab320851</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

LAMP1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 6:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 7:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa,36 kDa

false

Exposure time: 103s

Supplier Data

Western blot - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-LAMP1 antibody [RM1217] - Lysosome Marker (<a href='/en-us/products/primary-antibodies/lamp1-antibody-rm1217-lysosome-marker-ab320851'>ab320851</a>) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Mouse colon tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa,36 kDa

true

Exposure time: 103s

Supplier Data

Western blot - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

This data was developed using ab320851, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-LAMP1 antibody [RM1217] - Lysosome Marker (<a href='/en-us/products/primary-antibodies/lamp1-antibody-rm1217-lysosome-marker-ab320851'>ab320851</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa whole cell lysate treated with Peptide:N-glycosidase F (PNGase F) at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110-44 kDa,36 kDa

true

Exposure time: 180s

Unconjugated

Anti-LAMP1 antibody [RM1217] - Lysosome Marker

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RM1217

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

IP, Flow Cyt (Intra), IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab320852 is the carrier-free version of ab320851.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.

Biological function summary

LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.

Pathways

The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.

LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis (PubMed : 37390818). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed : 37390818). Also plays an important role in NK-cells cytotoxicity (PubMed : 2022921, PubMed : 23632890). Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule (PubMed : 23632890). In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content (PubMed : 23847195). Presents carbohydrate ligands to selectins (PubMed : 7685349).. (Microbial infection) Acts as a receptor for Lassa virus glycoprotein (PubMed : 24970085, PubMed : 25972533, PubMed : 27605678, PubMed : 28448640). Promotes also fusion of the virus with host membrane in less acidic endosomes (PubMed : 29295909).. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.

See full target information LAMP1

Additional targets

Lamp1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAMP1 antibody [RM1217] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunoprecipitation - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunocytochemistry/ Immunofluorescence - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Flow Cytometry (Intracellular) - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Immunoprecipitation - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Western blot - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Western blot - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Western blot - Anti-LAMP1 antibody [RM1217] - BSA and Azide free (AB320852)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise