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Anti-LAMP2 antibody [GL2A7] is a rat monoclonal antibody that is used to detect LAMP2 in Flow cytometry, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rabbit samples.

- Cited in over 205 publications
- Antibody clone GL2A7 has been tried and trusted by researchers since 2004 and is cited in >260 publications


Images

Western blot - Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (AB13524), expandable thumbnail
  • Western blot - Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (AB13524), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rat
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Tissue preparation containing Lamp2 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P17047

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIPFlow CytWBIHC-FoFrIHC-PICC/IFIHC-Fr
Human
Expected
Expected
Expected
Tested
Expected
Expected
Expected
Expected
Mouse
Expected
Expected
Expected
Tested
Expected
Expected
Expected
Expected
Rabbit
Expected
Expected
Expected
Predicted
Expected
Expected
Expected
Expected

Expected
Expected

Species
Mouse
Dilution info
-
Notes

Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.

Species
Human
Dilution info
-
Notes

Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.

Species
Rabbit
Dilution info
-
Notes

Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%.

Expected
Expected

Species
Mouse
Dilution info
-
Notes

(PMID 2142158).

Species
Human
Dilution info
-
Notes

(PMID 2142158).

Species
Rabbit
Dilution info
-
Notes

(PMID 2142158).

Expected
Expected

Species
Mouse
Dilution info
-
Notes

ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
-
Notes

ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Rabbit
Dilution info
-
Notes

ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Human
Dilution info
1/500
Notes

-

Predicted
Predicted

Species
Rabbit
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
-
Notes

(PubMed:17425404)

Species
Human
Dilution info
-
Notes

(PubMed:17425404)

Species
Rabbit
Dilution info
-
Notes

(PubMed:17425404)

Expected
Expected

Species
Mouse, Human, Rabbit
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Mouse
Dilution info
-
Notes

See Abreview.

Species
Human
Dilution info
-
Notes

See Abreview.

Species
Rabbit
Dilution info
-
Notes

See Abreview.

Expected
Expected

Species
Mouse, Human, Rabbit
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

The protein expressed by gene LAMP2 plays an important role in chaperone-mediated autophagy, facilitating the lysosomal degradation of proteins in response to various stresses and as part of normal protein turnover, especially for those with a long biological half-life. It functions by binding target proteins like GAPDH and MLLT11, directing them for lysosomal degradation, and is necessary for the fusion of autophagosomes with lysosomes during autophagy. Although cells lacking LAMP2 express normal VAMP8 levels, they fail to accumulate STX17 on autophagosomes, likely explaining the fusion failure between autophagosomes and lysosomes. LAMP2 is essential for the normal degradation of autophagosomal contents and efficient MHCII-mediated presentation of exogenous antigens, as it facilitates lysosomal protein degradation. It is not required for the presentation of endogenous antigens via MHCII. Specifically, isoform LAMP-2C modulates chaperone-mediated autophagy, reduces presentation of endogenous antigens by MHCII, and does not influence the presentation of exogenous and membrane-derived antigens by MHCII. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

Recommended products

Anti-LAMP2 antibody [GL2A7] is a rat monoclonal antibody that is used to detect LAMP2 in Flow cytometry, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rabbit samples.

- Cited in over 205 publications
- Antibody clone GL2A7 has been tried and trusted by researchers since 2004 and is cited in >260 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Tissue preparation containing Lamp2 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P17047
Clone number
GL2A7
Purification technique
Affinity purification Protein G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Product Specifications

Anti-LAMP2 antibody [GL2A7] (ab13524) is a rat monoclonal antibody and is validated for use in Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, WB in human, mouse, rabbit samples.
Anti-LAMP2 antibody [GL2A7] (ab13524) specifically detects LAMP2 (UniProt ID: P13473; Molecular weight: 42kDa) and is sold in 100 µL selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-LAMP2 antibody [GL2A7] (ab13524) has high sensitivity and specificity.
Anti-LAMP2 antibody [GL2A7] (ab13524) has been cited over 207 times in peer reviewed journals and is trusted by the scientific community.
Anti-LAMP2 antibody [GL2A7] (ab13524) has 9 independent reviews from customers.

For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAMP2 also known as CD107b is an important protein found in the lysosomal membrane. It plays a significant role in lysosome function facilitating autophagy and the degradation of cellular debris. LAMP2 proteins are expressed in most tissues especially in tissues with high lysosomal activity like the liver kidney and heart. The LAMP2 molecule has a molecular weight of approximately 45 kDa. Researchers frequently use LAMP2 staining and LAMP2 marker to study its cellular localization and expression levels.

Biological function summary

LAMP2 is essential for the normal fusion of lysosomes with autophagosomes. This process ensures the recycling of cellular components and maintenance of cellular homeostasis. LAMP2 acts in conjunction with other members of the lysosome-associated membrane protein family LAMP1 and LAMP3 forming part of a larger complex. This interaction is fundamental in managing the degradation pathways within the cell supporting processes such as lipid metabolism and protein turnover.

Pathways

The activity of LAMP2 is critical in the autophagic and degradation pathways within the cell. It particularly interacts in the autophagy-lysosome pathway where it aids the clearance of damaged organelles and protein aggregates. LAMP2 interaction with proteins such as the autophagy-related protein LC3 helps this degradation process. Additionally LAMP2 plays a role in endocytic pathways linking it to proteins involved in vesicle trafficking and membrane fusion.

Associated diseases and disorders

Mutations or deficiencies in LAMP2 are associated with Danon disease a type of lysosomal storage disorder. Patients with Danon disease exhibit symptoms such as cardiomyopathy skeletal myopathy and intellectual disabilities. LAMP2 is also implicated in other lysosomal disorders with a connection to the malfunction of autophagy processes. Its dysfunction is often linked with proteins responsible for cellular degradation affecting the normal homeostatic balance and leading to the accumulation of autophagic substrates.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Western blot - Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524)

    LAMP2 Western blot staining of HEK293 cell lysate using rat Anti-LAMP2 antibody

    Blocked with 5% milk for 1 hour at RT.

    Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.

    All lanes: Western blot - Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524) at 1/1000 dilution

    All lanes: HEK293 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-rat HRP at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa

    Observed band size: 105 kDa, 28 kDa, 70 kDa

    Exposure time: 3min

  • Western blot - Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524), expandable thumbnail

    Western blot - Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524)

    LAMP2 Western blot staining using rat Anti-LAMP2 antibody

    Block: 5% milk + TBST for 1 hour at RT.

    All lanes: Western blot - Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524) at 1/500 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg

    Lane 2: NIH/3T3 (Mouse embryo fibroblast cell line) lysate at 10 µg

    Secondary

    All lanes: HRP Goat Anti-Rat, 1 hour at RT at 1/100 dilution

    Predicted band size: 45 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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