Anti-LAMP2 antibody [GL2A7] is a rat monoclonal antibody that is used to detect LAMP2 in Flow cytometry, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rabbit samples.
- Cited in over 205 publications
- Antibody clone GL2A7 has been tried and trusted by researchers since 2004 and is cited in >260 publications
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
ICC | IP | Flow Cyt | WB | IHC-FoFr | IHC-P | ICC/IF | IHC-Fr | |
---|---|---|---|---|---|---|---|---|
Human | Expected | Expected | Expected | Tested | Expected | Expected | Expected | Expected |
Mouse | Expected | Expected | Expected | Tested | Expected | Expected | Expected | Expected |
Rabbit | Expected | Expected | Expected | Predicted | Expected | Expected | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%. |
Species Human | Dilution info - | Notes Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%. |
Species Rabbit | Dilution info - | Notes Fix cells with methanol or with paraformaldehyde-lysine-periodate formulation. If the latter is used, permeabilize with saponin, not with Triton X-100 or NP-40. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin 0.01%. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes (PMID 2142158). |
Species Human | Dilution info - | Notes (PMID 2142158). |
Species Rabbit | Dilution info - | Notes (PMID 2142158). |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rabbit | Dilution info - | Notes ab37371 - Rat polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes (PubMed:17425404) |
Species Human | Dilution info - | Notes (PubMed:17425404) |
Species Rabbit | Dilution info - | Notes (PubMed:17425404) |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes See Abreview. |
Species Human | Dilution info - | Notes See Abreview. |
Species Rabbit | Dilution info - | Notes See Abreview. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
The protein expressed by gene LAMP2 plays an important role in chaperone-mediated autophagy, facilitating the lysosomal degradation of proteins in response to various stresses and as part of normal protein turnover, especially for those with a long biological half-life. It functions by binding target proteins like GAPDH and MLLT11, directing them for lysosomal degradation, and is necessary for the fusion of autophagosomes with lysosomes during autophagy. Although cells lacking LAMP2 express normal VAMP8 levels, they fail to accumulate STX17 on autophagosomes, likely explaining the fusion failure between autophagosomes and lysosomes. LAMP2 is essential for the normal degradation of autophagosomal contents and efficient MHCII-mediated presentation of exogenous antigens, as it facilitates lysosomal protein degradation. It is not required for the presentation of endogenous antigens via MHCII. Specifically, isoform LAMP-2C modulates chaperone-mediated autophagy, reduces presentation of endogenous antigens by MHCII, and does not influence the presentation of exogenous and membrane-derived antigens by MHCII. This supplementary information is collated from multiple sources and compiled automatically.
CD107b, Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, LGP-96, LAMP2
Anti-LAMP2 antibody [GL2A7] is a rat monoclonal antibody that is used to detect LAMP2 in Flow cytometry, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, Western blot. Suitable for Human, Mouse, Rabbit samples.
- Cited in over 205 publications
- Antibody clone GL2A7 has been tried and trusted by researchers since 2004 and is cited in >260 publications
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Product Specifications
Anti-LAMP2 antibody [GL2A7] (ab13524) is a rat monoclonal antibody and is validated for use in Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, IP, WB in human, mouse, rabbit samples.
Anti-LAMP2 antibody [GL2A7] (ab13524) specifically detects LAMP2 (UniProt ID: P13473; Molecular weight: 42kDa) and is sold in 100 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-LAMP2 antibody [GL2A7] (ab13524) has high sensitivity and specificity.
Anti-LAMP2 antibody [GL2A7] (ab13524) has been cited over 207 times in peer reviewed journals and is trusted by the scientific community.
Anti-LAMP2 antibody [GL2A7] (ab13524) has 9 independent reviews from customers.
For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
LAMP2 also known as CD107b is an important protein found in the lysosomal membrane. It plays a significant role in lysosome function facilitating autophagy and the degradation of cellular debris. LAMP2 proteins are expressed in most tissues especially in tissues with high lysosomal activity like the liver kidney and heart. The LAMP2 molecule has a molecular weight of approximately 45 kDa. Researchers frequently use LAMP2 staining and LAMP2 marker to study its cellular localization and expression levels.
LAMP2 is essential for the normal fusion of lysosomes with autophagosomes. This process ensures the recycling of cellular components and maintenance of cellular homeostasis. LAMP2 acts in conjunction with other members of the lysosome-associated membrane protein family LAMP1 and LAMP3 forming part of a larger complex. This interaction is fundamental in managing the degradation pathways within the cell supporting processes such as lipid metabolism and protein turnover.
The activity of LAMP2 is critical in the autophagic and degradation pathways within the cell. It particularly interacts in the autophagy-lysosome pathway where it aids the clearance of damaged organelles and protein aggregates. LAMP2 interaction with proteins such as the autophagy-related protein LC3 helps this degradation process. Additionally LAMP2 plays a role in endocytic pathways linking it to proteins involved in vesicle trafficking and membrane fusion.
Mutations or deficiencies in LAMP2 are associated with Danon disease a type of lysosomal storage disorder. Patients with Danon disease exhibit symptoms such as cardiomyopathy skeletal myopathy and intellectual disabilities. LAMP2 is also implicated in other lysosomal disorders with a connection to the malfunction of autophagy processes. Its dysfunction is often linked with proteins responsible for cellular degradation affecting the normal homeostatic balance and leading to the accumulation of autophagic substrates.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
LAMP2 Western blot staining of HEK293 cell lysate using rat Anti-LAMP2 antibody
Blocked with 5% milk for 1 hour at RT.
Incubated with primary antibody in 5% BSA/TBST for 16 hours at 4°C.
All lanes: Western blot - Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524) at 1/1000 dilution
All lanes: HEK293 cell lysate at 20 µg
All lanes: Goat anti-rat HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 105 kDa, 28 kDa, 70 kDa
Exposure time: 3min
LAMP2 Western blot staining using rat Anti-LAMP2 antibody
Block: 5% milk + TBST for 1 hour at RT.
All lanes: Western blot - Anti-LAMP2 antibody [GL2A7] - Lysosome Marker (ab13524) at 1/500 dilution
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate at 20 µg
Lane 2: NIH/3T3 (Mouse embryo fibroblast cell line) lysate at 10 µg
All lanes: HRP Goat Anti-Rat, 1 hour at RT at 1/100 dilution
Predicted band size: 45 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com