Anti-LANCL2 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LANCL2 antibody (AB237520)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for LANCL2 (Green) using ab237520 at 1/100 dilution in ICC/IF, followed by Alexa Fluor^® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LANCL2 antibody (AB237520)

Paraffin-embedded human brain tissue stained for LANCL2 using ab237520 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• WB

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Western blot - Anti-LANCL2 antibody (AB237520)

All lanes:

Western blot - Anti-LANCL2 antibody (ab237520) at 1/500 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate

Lane 2:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 51 kDa

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