Rabbit Recombinant Monoclonal LANCL2 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Images
![Dot Blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (AB317445), expandable thumbnail](https://content.abcam.com/products/images/lancl2-antibody-epr27973-132-bsa-and-azide-free-ab317445--dot-blot-img431560.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (AB317445)")
![Western blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (AB317445), expandable thumbnail](https://content.abcam.com/products/images/lancl2-antibody-epr27973-132-bsa-and-azide-free-ab317445--western-blot-img431562.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (AB317445)")
![Western blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (AB317445), expandable thumbnail](https://content.abcam.com/products/images/lancl2-antibody-epr27973-132-bsa-and-azide-free-ab317445--western-blot-img431561.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (AB317445)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | IHC-P | Flow Cyt (Intra) | IP | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Expected | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Expected | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Necessary for abscisic acid (ABA) binding on the cell membrane and activation of the ABA signaling pathway in granulocytes.
Alternative names
GPR69B, TASP, LANCL2, LanC-like protein 2, Testis-specific adriamycin sensitivity protein
Recommended products
Rabbit Recombinant Monoclonal LANCL2 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27973-132
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317445 is the carrirer-free version of Anti-LANCL2 antibody [EPR27973-132] ab317444.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LANCL2 also known as Lanthionine Synthetase C-Like 2 is a protein with a molecular weight of approximately 44 kDa. It belongs to the LANCL protein family which also includes LANCL1 and LANCL3. LANCL2 is expressed in various tissues with significant expression observed in adipose tissue liver and skeletal muscle. Its intracellular location suggests a role in cellular processes that require close interaction with intracellular signaling pathways.
- Biological function summary
LANCL2 functions in regulatory mechanisms related to metabolic processes. It does not exist in isolation; instead it interacts with other proteins as part of a multiprotein complex. LANCL2 modulates the signaling pathway involving the peroxisome proliferator-activated receptor gamma (PPARγ) and plays a role in modulating glucose and lipid metabolism. Its activity suggests involvement in maintaining cellular energy balance and homeostasis.
- Pathways
LANCL2 is integrated into metabolic pathways with significant involvement in both the PPAR signaling pathway and the insulin signaling pathway. These pathways highlight its importance in glucose homeostasis and energy metabolism. LANCL2 interacts with proteins such as AKT an important player in the insulin signaling pathway contributing to the regulation of glucose uptake and lipid metabolism.
- Associated diseases and disorders
LANCL2 has been connected to metabolic diseases particularly type 2 diabetes and obesity. Changes in LANCL2 expression or function can impact glucose and lipid regulation contributing to these conditions. LANCL2's role in these disorders often involves its interaction with proteins like PPARγ which also influences lipid storage and insulin sensitivity. Understanding LANCL2's role in these diseases could provide insights into novel therapeutic approaches.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Dot Blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (ab317445), expandable thumbnail](https://content.abcam.com/products/images/lancl2-antibody-epr27973-132-bsa-and-azide-free-ab317445--dot-blot-img431560.jpg "Dot Blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (ab317445)")
Dot Blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (ab317445)
LANCL2 Dot Blot staining using rabbit Anti-LANCL2 antibody
This data was developed using Anti-LANCL2 antibody [EPR27973-132] ab317444, the same antibody clone in a different buffer formulation.
Dot blot analysis of LANCL2 using Anti-LANCL2 antibody [EPR27973-132] ab317444 at 1:1000 (0.512 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Exposure time: 180seconds.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human LANCL1.
All lanes: Dot Blot - Anti-LANCL2 antibody [EPR27973-132] (Anti-LANCL2 antibody [EPR27973-132] ab317444) at 1/1000 dilution
Lane 1: Human LANCL1 protein
Lane 2: Human LANCL2 protein
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Western blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (ab317445), expandable thumbnail](https://content.abcam.com/products/images/lancl2-antibody-epr27973-132-bsa-and-azide-free-ab317445--western-blot-img431562.jpg "Western blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (ab317445)")
Western blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (ab317445)
This data was developed using Anti-LANCL2 antibody [EPR27973-132] ab317444, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Skeletal muscle, spleen, lung(PMID: 11762191)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-LANCL2 antibody [EPR27973-132] (Anti-LANCL2 antibody [EPR27973-132] ab317444) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: Mouse lung tissue lysate at 20 µg with 5% NFDM/TBST
Lane 6: Rat brain tissue lysate at 20 µg with 5% NFDM/TBST
Lane 7: Rat testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 8: Rat spleen tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 50 kDa, 36 kDa
Exposure time: 180s
![Western blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (ab317445), expandable thumbnail](https://content.abcam.com/products/images/lancl2-antibody-epr27973-132-bsa-and-azide-free-ab317445--western-blot-img431561.jpg "Western blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (ab317445)")
Western blot - Anti-LANCL2 antibody [EPR27973-132] - BSA and Azide free (ab317445)
This data was developed using Anti-LANCL2 antibody [EPR27973-132] ab317444, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Skeletal muscle, spleen(PMID: 11762191), SK-MEL-28
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-LANCL2 antibody [EPR27973-132] (Anti-LANCL2 antibody [EPR27973-132] ab317444) at 1/1000 dilution
Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: SK-MEL-28 (human malignant melanoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human hippocampus tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: Human spleen tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
Observed band size: 50 kDa, 36 kDa
Exposure time: 180s
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