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AB192027

Anti-Langerin antibody [EPR15863]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • 20ul selling size
  • What is this?

5

(1 Review)

|

(9 Publications)

Anti-Langerin antibody [EPR15863] (ab192027) is a rabbit monoclonal antibody detecting Langerin in IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD207, CLEC4K, C-type lectin domain family 4 member K, Langerin

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR15863] (AB192027)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR15863] (AB192027)

Immunohistochemical analysis of Paraffin-embedded sections of human skin tissue labelling Langerin with ab192027 at 1/1000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the Langerhans cells in the human skin is observed. Counter-stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

The section was incubated with ab192027 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR15863] (AB192027)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR15863] (AB192027)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Langerin with ab192027 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilized PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15863

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-Langerin antibody [EPR15863] (ab192027) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR15863] also available for your convenience: ab192027, Alexa Fluor® 488 - ab225374, Alexa Fluor® 647 - ab225375, Carrier free - ab232603, Alexa Fluor® 555 - ab274894, Alexa Fluor® 594 - ab275353, Biotin - ab322985

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Langerin also known as CD207 is a type II transmembrane protein involved in the immune response. It weighs approximately 40 kDa and is expressed mainly in Langerhans cells a specialized subset of dendritic cells found in the skin and mucosal tissues. Langerin functions as a C-type lectin receptor with the ability to recognize and internalize glycosylated antigens. This target facilitates the capture and presentation of antigens enhancing the immune surveillance process.
Biological function summary

Langerin supports Langerhans cells in the recognition and processing of pathogens. The protein forms homotrimers enabling it to bind specific mannose-rich glycan structures on pathogens such as viruses and bacteria. Through its endocytic properties Langerin internalizes these antigens into Birbeck granules unique organelles in Langerhans cells promoting antigen processing and presentation to T cells therefore initiating immune responses.

Pathways

Langerin is critical for antigen uptake and processing via the lectin pathway of endocytosis. It interacts with other dendritic cell receptors and influences signaling pathways that enhance immune activation such as the Syk and Raf-1 kinase pathways. Langerin's ability to internalize antigens connects it with proteins like DC-SIGN and MR which share roles in pathogen recognition and immune modulation.

Langerin plays a significant role in conditions like Langerhans Cell Histiocytosis (LCH) and HIV. In LCH abnormal proliferation of Langerhans cells occurs where Langerin expression helps in diagnosing and characterizing LCH lesions. In the context of HIV Langerin interacts with viral particles reducing their ability to infect immune cells. This protein also relates to other immune receptors like CD40 and CD86 which regulate immune responses in associated disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. Major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. Protects against human immunodeficiency virus-1 (HIV-1) infection. Binds to high-mannose structures present on the envelope glycoprotein which is followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation.
See full target information CD207
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical medicine 14: PubMed40565944

2025

Pyodermatitis-Pyostomatitis Vegetans: The Role of Langerin Deficiency in Disease Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Pan,Jiongke Wang,Luyao Cai,Mei Huang,Qi Han,Zhijian Zheng,Xin Zeng,Qianming Chen,Ruixue Ai,Yu Zhou

Frontiers in molecular biosciences 10:1215185 PubMed37711386

2023

Corrigendum: Dendritic cell proliferation by primary cilium in atopic dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Manami Toriyama,Defri Rizaldy,Motoki Nakamura,Yukiko Atsumi,Michinori Toriyama,Fumitaka Fujita,Fumihiro Okada,Akimichi Morita,Hiroshi Itoh,Ken J Ishii

Frontiers in molecular biosciences 10:1149828 PubMed37179569

2023

Dendritic cell proliferation by primary cilium in atopic dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Manami Toriyama,Defri Rizaldy,Motoki Nakamura,Yukiko Atsumi,Michinori Toriyama,Fumitaka Fujita,Fumihiro Okada,Akimichi Morita,Hiroshi Itoh,Ken J Ishii

Parasite immunology 45:e12971 PubMed36695719

2023

Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen M Sandoval Pacheco,Gabriela V Araujo Flores,Aurea F Ferreira,Vânia L R da Matta,Claudia M de Castro Gomes,Wilfredo H Sosa-Ochoa,Concepción Zúniga,Fernando T Silveira,Carlos E P Corbett,Márcia D Laurenti

Pharmaceutics 13: PubMed33917842

2021

Pre-Emptive Priming of Human Skin Improves Cutaneous Scarring and Is Superior to Immediate and Delayed Topical Anti-Scarring Treatment Post-Wounding: A Double-Blind Randomised Placebo-Controlled Clinical Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Ud-Din,Traci A Wilgus,Douglas D McGeorge,Ardeshir Bayat

Experimental dermatology 30:249-261 PubMed33067891

2020

Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL-36γ and IL-37.

Applications

Unspecified application

Species

Unspecified reactive species

Astrid H Lossius,Teresa L Berents,Frank Saetre,Hogne R Nilsen,Maria Bradley,Samina Asad,Guttorm Haraldsen,Olav Sundnes,Jan-Øivind Holm

The Journal of investigative dermatology 139:1680-1690.e16 PubMed30822414

2019

A Double-Blind, Randomized Trial Shows the Role of Zonal Priming and Direct Topical Application of Epigallocatechin-3-Gallate in the Modulation of Cutaneous Scarring in Human Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Ud-Din,Philip Foden,Mohsin Mazhari,Samer Al-Habba,Mohamed Baguneid,Silvia Bulfone-Paus,Douglas McGeorge,Ardeshir Bayat

The American journal of pathology 187:1380-1398 PubMed28432872

2017

Human Effector Memory T Helper Cells Engage with Mouse Macrophages and Cause Graft-versus-Host-Like Pathology in Skin of Humanized Mice Used in a Nonclinical Immunization Study.

Applications

Unspecified application

Species

Unspecified reactive species

Balasai Sundarasetty,Valery Volk,Sebastian J Theobald,Susanne Rittinghausen,Dirk Schaudien,Vanessa Neuhaus,Constanca Figueiredo,Andreas Schneider,Laura Gerasch,Adele Mucci,Thomas Moritz,Constantin von Kaisenberg,Loukia M Spineli,Katherina Sewald,Armin Braun,Henning Weigt,Arnold Ganser,Renata Stripecke

The Journal of investigative dermatology 137:1588-1590 PubMed28365260

2017

CXCL12 as a Predictor of Vitiligo Activity and Disease Progression.

Applications

IHC-P

Species

Unspecified reactive species

Vitali Alexeev
View all publications
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Product promise

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