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Rabbit Recombinant Monoclonal Langerin antibody. Suitable for IHC-P, IHC-Fr, mIHC and reacts with Mouse, Human samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (AB283686), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (AB283686), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (AB283686), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Langerin antibody [EPR24685-12] (AB283686), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Langerin antibody [EPR24685-12] (AB283686), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PIHC-FrFlow Cyt (Intra)mIHC
Human
Not recommended
Not recommended
Tested
Expected
Not recommended
Tested
Mouse
Not recommended
Not recommended
Tested
Tested
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

-

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/1000

Notes

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Target data

Function

Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells. Major receptor on primary Langerhans cells for Candida species, Saccharomyces species, and Malassezia furfur. Protects against human immunodeficiency virus-1 (HIV-1) infection. Binds to high-mannose structures present on the envelope glycoprotein which is followed by subsequent targeting of the virus to the Birbeck granules leading to its rapid degradation.

Alternative names

CLEC4K, CLEC4K, CD207, C-type lectin domain family 4 member K, Langerin

Recommended products

Rabbit Recombinant Monoclonal Langerin antibody. Suitable for IHC-P, IHC-Fr, mIHC and reacts with Mouse, Human samples. Cited in 1 publication.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR24685-12

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Langerin also known as CD207 is a type II transmembrane protein involved in the immune response. It weighs approximately 40 kDa and is expressed mainly in Langerhans cells a specialized subset of dendritic cells found in the skin and mucosal tissues. Langerin functions as a C-type lectin receptor with the ability to recognize and internalize glycosylated antigens. This target facilitates the capture and presentation of antigens enhancing the immune surveillance process.

Biological function summary

Langerin supports Langerhans cells in the recognition and processing of pathogens. The protein forms homotrimers enabling it to bind specific mannose-rich glycan structures on pathogens such as viruses and bacteria. Through its endocytic properties Langerin internalizes these antigens into Birbeck granules unique organelles in Langerhans cells promoting antigen processing and presentation to T cells therefore initiating immune responses.

Pathways

Langerin is critical for antigen uptake and processing via the lectin pathway of endocytosis. It interacts with other dendritic cell receptors and influences signaling pathways that enhance immune activation such as the Syk and Raf-1 kinase pathways. Langerin’s ability to internalize antigens connects it with proteins like DC-SIGN and MR which share roles in pathogen recognition and immune modulation.

Associated diseases and disorders

Langerin plays a significant role in conditions like Langerhans Cell Histiocytosis (LCH) and HIV. In LCH abnormal proliferation of Langerhans cells occurs where Langerin expression helps in diagnosing and characterizing LCH lesions. In the context of HIV Langerin interacts with viral particles reducing their ability to infect immune cells. This protein also relates to other immune receptors like CD40 and CD86 which regulate immune responses in associated disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (ab283686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (ab283686)

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling Langerin with ab283686 at 1/500 (1.066 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on Langerhans cells in human skin. The section was incubated with ab283686 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (ab283686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (ab283686)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling Langerin with ab283686 at 1/500 (1.066 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on scattered cells in human lung carcinoma. The section was incubated with ab283686 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (ab283686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (ab283686)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling Langerin with ab283686 at 1/500 (1.066 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on human cerebrum. The section was incubated with ab283686 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Langerin antibody [EPR24685-12] (ab283686), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Langerin antibody [EPR24685-12] (ab283686)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse thymus (fresh) tissue labeling Langerin with ab283686 at 1/100 (5.33 μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green). Positive staining on mouse thymus is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 μg/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Langerin antibody [EPR24685-12] (ab283686), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Langerin antibody [EPR24685-12] (ab283686)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skin (fresh) tissue labeling Langerin with ab283686 at 1/100 (5.33 μg/mL) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green). Positive staining on Langerhans cells of mouse skin is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 μg/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (ab283686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (ab283686)

    Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labelling Langerin with ab283686 at 1/500 (1.066 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on Langerhans cells in mouse skin. The section was incubated with ab283686 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (ab283686), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] (ab283686)

    Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labelling Langerin with ab283686 at 1/500 (1.066 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on scattered immune cells in mouse thymic medulla. The section was incubated with ab283686 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-Langerin antibody [EPR24685-12] (ab283686), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Langerin antibody [EPR24685-12] (ab283686)

    Panel A: merged staining of anti-Desmoglein 1/DSG1 (gray; Opal™690), anti-Langerin (green; Opal™520) and anti-TRP2/DCT (red; Opal™570) on human skin.
    Panel B: anti-TRP2/DCT stained on melanocytes.
    Panel C: anti-Langerin stained on Langerhans cells.
    Panel D: anti-Desmoglein 1/DSG1 stained on epidermis.

    The section was incubated in three rounds of staining: in the order of Anti-Desmoglein 1/DSG1 antibody [EPR20382] - BSA and Azide free ab236259, ab283686, and Anti-TRP2/DCT antibody [EPR21986] ab221144 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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