Anti-Langerin antibody [EPR24685-12] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] - BSA and Azide free (AB283714)

This data was developed using ab283686, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skin tissue labelling Langerin with ab283686 at 1/500 (1.066 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on Langerhans cells in human skin. The section was incubated with ab283686 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] - BSA and Azide free (AB283714)

This data was developed using ab283686, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling Langerin with ab283686 at 1/500 (1.066 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on human cerebrum. The section was incubated with ab283686 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Langerin antibody [EPR24685-12] - BSA and Azide free (AB283714)

This data was developed using ab283686, the same antibody clone in a different buffer formulation. Panel A : merged staining of anti-Desmoglein 1/DSG1 (gray; Opal™690), anti-Langerin (green; Opal™520) and anti-TRP2/DCT (red; Opal™570) on human skin. Panel B : anti-TRP2/DCT stained on melanocytes. Panel C : anti-Langerin stained on Langerhans cells. Panel D : anti-Desmoglein 1/DSG1 stained on epidermis. The section was incubated in three rounds of staining : in the order of ab236259, ab283686, and ab221144 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] - BSA and Azide free (AB283714)

This data was developed using ab283686, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling Langerin with ab283686 at 1/500 (1.066 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on scattered cells in human lung carcinoma. The section was incubated with ab283686 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Langerin antibody [EPR24685-12] - BSA and Azide free (AB283714)

This data was developed using ab283686, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse thymus (fresh) tissue labeling Langerin with ab283686 at 1/100 (5.33 μg/mL) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green). Positive staining on mouse thymus is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 μg/mL dilution.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Langerin antibody [EPR24685-12] - BSA and Azide free (AB283714)

This data was developed using ab283686, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skin staining of Cytokeratin 1 + Cytokeratin 2e with ab315228 at a 1/2000 (0.263 μg/ml) dilution, Collagen I with ab270993 and Langerin with ab283686 at a 1/1000 dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Cytokeratin 1 + Cytokeratin 2e (green; Opal™520), anti-Collagen I (magenta; Opal™690) and anti-Langerin (gray; Opal™570) on mouse skin.
Panel B : anti-Cytokeratin 1 + Cytokeratin 2e staining epidermis keratin layer in mouse skin.
Panel C : anti-Collagen I staining connective tissues in mouse skin.
Panel D : anti-Langerin staining Langerhans cells in mouse skin.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab315228, ab270993 and ab283686 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system,

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] - BSA and Azide free (AB283714)

This data was developed using ab283686, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labelling Langerin with ab283686 at 1/500 (1.066 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on Langerhans cells in mouse skin. The section was incubated with ab283686 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR24685-12] - BSA and Azide free (AB283714)

This data was developed using ab283686, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labelling Langerin with ab283686 at 1/500 (1.066 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on scattered immune cells in mouse thymic medulla. The section was incubated with ab283686 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Langerin antibody [EPR24685-12] - BSA and Azide free (AB283714)

This data was developed using ab283686, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skin (fresh) tissue labeling Langerin with ab283686 at 1/100 (5.33 μg/mL) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green). Positive staining on Langerhans cells of mouse skin is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 μg/mL dilution.