Skip to main content

Rabbit Recombinant Monoclonal Langerin antibody. Suitable for WB, IP, IHC-P and reacts with Transfected cell lysate - Mouse, Mouse samples.

Be the first to review this product! Submit a review

Images

Multiplex immunohistochemistry - Anti-Langerin antibody [EPR28089-89] (AB317813), expandable thumbnail
  • Western blot - Anti-Langerin antibody [EPR28089-89] (AB317813), expandable thumbnail
  • Western blot - Anti-Langerin antibody [EPR28089-89] (AB317813), expandable thumbnail
  • Immunoprecipitation - Anti-Langerin antibody [EPR28089-89] (AB317813), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR28089-89] (AB317813), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Mouse
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Mouse
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Mouse, Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate - Mouse, Human, Rat
Dilution info
-
Notes

-

Target data

Function

Calcium-dependent lectin displaying mannose-binding specificity. Induces the formation of Birbeck granules (BGs); is a potent regulator of membrane superimposition and zippering. Binds to sulfated as well as mannosylated glycans, keratan sulfate (KS) and beta-glucans. Facilitates uptake of antigens and is involved in the routing and/or processing of antigen for presentation to T cells.

Alternative names

CD207, Clec4k, Cd207, C-type lectin domain family 4 member K, Langerin

Recommended products

Rabbit Recombinant Monoclonal Langerin antibody. Suitable for WB, IP, IHC-P and reacts with Transfected cell lysate - Mouse, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28089-89
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Langerin also known as CD207 is a type II transmembrane protein involved in the immune response. It weighs approximately 40 kDa and is expressed mainly in Langerhans cells a specialized subset of dendritic cells found in the skin and mucosal tissues. Langerin functions as a C-type lectin receptor with the ability to recognize and internalize glycosylated antigens. This target facilitates the capture and presentation of antigens enhancing the immune surveillance process.

Biological function summary

Langerin supports Langerhans cells in the recognition and processing of pathogens. The protein forms homotrimers enabling it to bind specific mannose-rich glycan structures on pathogens such as viruses and bacteria. Through its endocytic properties Langerin internalizes these antigens into Birbeck granules unique organelles in Langerhans cells promoting antigen processing and presentation to T cells therefore initiating immune responses.

Pathways

Langerin is critical for antigen uptake and processing via the lectin pathway of endocytosis. It interacts with other dendritic cell receptors and influences signaling pathways that enhance immune activation such as the Syk and Raf-1 kinase pathways. Langerin’s ability to internalize antigens connects it with proteins like DC-SIGN and MR which share roles in pathogen recognition and immune modulation.

Associated diseases and disorders

Langerin plays a significant role in conditions like Langerhans Cell Histiocytosis (LCH) and HIV. In LCH abnormal proliferation of Langerhans cells occurs where Langerin expression helps in diagnosing and characterizing LCH lesions. In the context of HIV Langerin interacts with viral particles reducing their ability to infect immune cells. This protein also relates to other immune receptors like CD40 and CD86 which regulate immune responses in associated disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Multiplex immunohistochemistry - Anti-Langerin antibody [EPR28089-89] (ab317813), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Langerin antibody [EPR28089-89] (ab317813)

    Multiplex immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of mouse skin tissue staining with Anti-Collagen VII antibody [EPR27012-157] ab309143 at 1/100 dilution, ab317813 at 1/500 dilution, and Anti-Loricrin antibody [EPR28296-23] ab315806 at 1/500 dilution. The secondary used was Opal Polymer HRP Ms + Rb. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
    Panel A: merged staining of anti-Collagen VII (green; Opal™520), anti-Langerin (gray; Opal™570) and anti-Loricrin (magenta; Opal™690) on mouse skin.
    Panel B: anti-Collagen VII staining the dermal–epidermal junction in mouse skin.
    Panel C: anti-Langerin staining Langerhans cells in mouse skin.
    Panel D: anti-Loricrin staining terminally differentiated epidermal cells in mouse skin.
    Nuclear DNA was labeled with DAPI (shown in blue).
    The section was incubated in three rounds of staining: in the order of Anti-Collagen VII antibody [EPR27012-157] ab309143, ab317813 and Anti-Loricrin antibody [EPR28296-23] ab315806 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Western blot - Anti-Langerin antibody [EPR28089-89] (ab317813), expandable thumbnail

    Western blot - Anti-Langerin antibody [EPR28089-89] (ab317813)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Langerin antibody [EPR28089-89] (ab317813) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containi a His-tag, whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: 293T cells transfected with a mouse CD207 expression vector containing a His-tag, whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 40-50 kDa, 36 kDa

    Exposure time: 15s

  • Western blot - Anti-Langerin antibody [EPR28089-89] (ab317813), expandable thumbnail

    Western blot - Anti-Langerin antibody [EPR28089-89] (ab317813)

    Negative control: Mouse liver and kidney

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Langerin antibody [EPR28089-89] (ab317813) at 1/1000 dilution

    Lane 1: Mouse lymph node tissue lysate at 20 µg with NFDM/TBST

    Lane 2: Mouse thymus tissue lysate at 20 µg with NFDM/TBST

    Lane 3: Mouse spleen tissue lysate at 20 µg with NFDM/TBST

    Lane 4: Mouse liver tissue lysate at 20 µg with NFDM/TBST

    Lane 5: Mouse kidney tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 40-50 kDa, 36 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-Langerin antibody [EPR28089-89] (ab317813), expandable thumbnail

    Immunoprecipitation - Anti-Langerin antibody [EPR28089-89] (ab317813)

    Langerin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab317813 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317813 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse spleen tissue lysate

    Lane 2: ab317813 IP in Mouse spleen tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317813 in mouse spleen tissue lysate

    All lanes: Immunoprecipitation - Anti-Langerin antibody [EPR28089-89] (ab317813) at 1/30 dilution

    All lanes: Mouse spleen tissue lysate with NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR28089-89] (ab317813), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR28089-89] (ab317813)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Langerin with ab317813 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse spleen.

    The section was incubated with ab317813 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR28089-89] (ab317813), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR28089-89] (ab317813)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Langerin with ab317813 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse liver.

    The section was incubated with ab317813 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR28089-89] (ab317813), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Langerin antibody [EPR28089-89] (ab317813)

    Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling Langerin with ab317813 at 1/500 (1.026 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on Langerhans cells of mouse skin.

    The section was incubated with ab317813 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com