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AB316366

Anti-LAP2 alpha antibody [3A3]

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Mouse Monoclonal LAP2 alpha antibody. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

LAP2, TMPO, Thymopoietin isoform alpha, Thymopoietin-related peptide isoform alpha, TP alpha, TPRP isoform alpha

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha Antibody [3A3] (AB316366)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha Antibody [3A3] (AB316366)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab316366 (red).

Western blot - Anti-LAP2 alpha Antibody [3A3] (AB316366)
  • WB

Supplier Data

Western blot - Anti-LAP2 alpha Antibody [3A3] (AB316366)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were boiled in Laemmli buffer and the proteins were separated by 8% SDS-PAGE and blotted onto nitrocellulose membrane. The membrane was blocked in 3% NFDM in PBS-T for 1h at RT and incubated at 4°C o/n with ab316366 at the indicated dilution in 0.5% NFDM/PBS-T. After incubation with anti-mouse Fc-HRP coupled secondary antibody for 1h at RT, ECL was performed with the Western Lightning reagents. A 30 second exposure is shown.

All lanes:

Western blot - Anti-LAP2 alpha Antibody [3A3] (ab316366) at 1 µg/mL

All lanes:

HeLa cell lysate

Secondary

All lanes:

anti-mouse Fc-HRP coupled secondary antibody

true

Exposure time: 30s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A3

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lamina-associated polypeptide 2 alpha (LAP2 alpha) also known as LAP2A is part of the LAP2 protein family. It has a molecular mass of approximately 76 kDa. This protein connects with the nuclear lamina and is prominently expressed in the nucleus of most cell types. LAP2 alpha interacts with chromatin and its mechanical role involves regulating interactions between chromatin and nuclear structures.
Biological function summary

LAP2 alpha contributes to processes like nuclear stability and chromatin organization. It is involved in the formation of a complex with lamin A/C facilitating the association of this nuclear lamina protein. This interaction plays a significant role in dynamically organizing the nuclear architecture throughout the cell cycle influencing cell differentiation and proliferation.

Pathways

The interaction of LAP2 alpha with lamin A/C places it in the context of the nuclear envelope dynamics. Key pathways include the regulation of genome stability and DNA repair pathways. The interaction with lamin A/C influences how cells manage genome maintenance making LAP2 alpha important for cellular homeostasis.

LAP2 alpha shows relevance to muscular dystrophy and progeria. LAP2 alpha’s interaction with lamin A/C is important because mutations in lamin A/C are known to cause Emery-Dreifuss muscular dystrophy. Additionally LAP2 alpha's role in nuclear architecture is significant in the context of Hutchinson-Gilford progeria syndrome where abnormalities in nuclear shape are evident. In these diseases LAP2 alpha's interaction or malfunction can lead to or exacerbate pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Plays an important role, together with LMNA, in the nuclear anchorage of RB1.. TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.
See full target information TMPO
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