JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB66588

Anti-LAP2 alpha antibody [3A3]

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Monoclonal LAP2 alpha antibody 3A3. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

LAP2, TMPO, Thymopoietin isoform alpha, Thymopoietin-related peptide isoform alpha, TP alpha, TPRP isoform alpha

1 Images
Western blot - Anti-LAP2 alpha antibody [3A3] (AB66588)
  • WB

Unknown

Western blot - Anti-LAP2 alpha antibody [3A3] (AB66588)

All lanes:

Western blot - Anti-LAP2 alpha antibody [3A3] (ab66588) at 10 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HeLa (post mitotic stage) Whole Cell Lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 75 kDa

Observed band size: 55 kDa,77 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A3

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB5162

Anti-LAP2 alpha antibody

Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody (AB5162)

  • 4
  • 1
Primary Antibodies

AB226336

Anti-LAP2 alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 alpha antibody (AB226336)

  • 0
  • 0
Primary Antibodies

AB226342

Anti-LAP2 alpha antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 alpha antibody - N-terminal (AB226342)

  • 0
  • 0
Primary Antibodies

AB189993

Anti-LAP2 alpha antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody - N-terminal (AB189993)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB32535

Anti-Lamin B Receptor/LBR antibody [E398L]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lamin B Receptor/LBR antibody [E398L] (AB32535)

  • 3
  • 3
Primary Antibodies

AB16048

Anti-Lamin B1 antibody - Nuclear Envelope Marker

BOND RX™ Validated|KO Validated|Lab Essentials

Western blot - Anti-Lamin B1 antibody - Nuclear Envelope Marker (AB16048)

  • 5
  • 110
Primary Antibodies

AB108595

Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Lamin A + Lamin C antibody [EPR4100] - Nuclear Envelope Marker (AB108595)

  • 5
  • 9
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lamina-associated polypeptide 2 alpha (LAP2 alpha) also known as LAP2A is part of the LAP2 protein family. It has a molecular mass of approximately 76 kDa. This protein connects with the nuclear lamina and is prominently expressed in the nucleus of most cell types. LAP2 alpha interacts with chromatin and its mechanical role involves regulating interactions between chromatin and nuclear structures.
Biological function summary

LAP2 alpha contributes to processes like nuclear stability and chromatin organization. It is involved in the formation of a complex with lamin A/C facilitating the association of this nuclear lamina protein. This interaction plays a significant role in dynamically organizing the nuclear architecture throughout the cell cycle influencing cell differentiation and proliferation.

Pathways

The interaction of LAP2 alpha with lamin A/C places it in the context of the nuclear envelope dynamics. Key pathways include the regulation of genome stability and DNA repair pathways. The interaction with lamin A/C influences how cells manage genome maintenance making LAP2 alpha important for cellular homeostasis.

LAP2 alpha shows relevance to muscular dystrophy and progeria. LAP2 alpha’s interaction with lamin A/C is important because mutations in lamin A/C are known to cause Emery-Dreifuss muscular dystrophy. Additionally LAP2 alpha's role in nuclear architecture is significant in the context of Hutchinson-Gilford progeria syndrome where abnormalities in nuclear shape are evident. In these diseases LAP2 alpha's interaction or malfunction can lead to or exacerbate pathology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Plays an important role, together with LMNA, in the nuclear anchorage of RB1.. TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.
See full target information TMPO
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature 561:551-555 PubMed30232450

2018

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Applications

Unspecified application

Species

Unspecified reactive species

Shiwei Liu,Mijung Kwon,Mark Mannino,Nachen Yang,Fioranna Renda,Alexey Khodjakov,David Pellman

Journal of cell science 128:2830-41 PubMed26092935

2015

The lamin-A/C-LAP2α-BAF1 protein complex regulates mitotic spindle assembly and positioning.

Applications

Unspecified application

Species

Unspecified reactive species

Ran Qi,Nan Xu,Gang Wang,He Ren,Si Li,Jun Lei,Qiaoyu Lin,Lihao Wang,Xin Gu,Hongyin Zhang,Qing Jiang,Chuanmao Zhang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com