Rabbit Polyclonal LAP2 alpha antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication.
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Publications
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- International journal of biological sciences 19:1528-15422023SIRT7 Downregulation Promotes Breast Cancer Metastasis Via LAP2α-Induced Chromosomal Instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Huo,Siqi Chen,Jialang Zhuang,Chuntao Quan,Yue Wang,Ni XiePubMed 37056924
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P42167
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Plays an important role, together with LMNA, in the nuclear anchorage of RB1. TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.
Alternative names
LAP2, TMPO, Thymopoietin isoform alpha, Thymopoietin-related peptide isoform alpha, TP alpha, TPRP isoform alpha
Recommended products
Rabbit Polyclonal LAP2 alpha antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information. Database link P42167
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab226336 was affinity purified using an epitope specific to LAP2 alpha immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Lamina-associated polypeptide 2 alpha (LAP2 alpha) also known as LAP2A is part of the LAP2 protein family. It has a molecular mass of approximately 76 kDa. This protein connects with the nuclear lamina and is prominently expressed in the nucleus of most cell types. LAP2 alpha interacts with chromatin and its mechanical role involves regulating interactions between chromatin and nuclear structures.
- Biological function summary
LAP2 alpha contributes to processes like nuclear stability and chromatin organization. It is involved in the formation of a complex with lamin A/C facilitating the association of this nuclear lamina protein. This interaction plays a significant role in dynamically organizing the nuclear architecture throughout the cell cycle influencing cell differentiation and proliferation.
- Pathways
The interaction of LAP2 alpha with lamin A/C places it in the context of the nuclear envelope dynamics. Key pathways include the regulation of genome stability and DNA repair pathways. The interaction with lamin A/C influences how cells manage genome maintenance making LAP2 alpha important for cellular homeostasis.
- Associated diseases and disorders
LAP2 alpha shows relevance to muscular dystrophy and progeria. LAP2 alpha’s interaction with lamin A/C is important because mutations in lamin A/C are known to cause Emery-Dreifuss muscular dystrophy. Additionally LAP2 alpha's role in nuclear architecture is significant in the context of Hutchinson-Gilford progeria syndrome where abnormalities in nuclear shape are evident. In these diseases LAP2 alpha's interaction or malfunction can lead to or exacerbate pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 alpha antibody (ab226336)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for LAP2 alpha using ab226336 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
Immunoprecipitation - Anti-LAP2 alpha antibody (ab226336)
LAP2 alpha was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226336 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226336 at 1 μg/ml.
Lane 1: ab226336 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-LAP2 alpha antibody (ab226336)
Predicted band size: 75 kDa
Western blot - Anti-LAP2 alpha antibody (ab226336)
Cell lysates were prepared using NETN lysis buffer.
All lanes: Western blot - Anti-LAP2 alpha antibody (ab226336) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysates at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysates at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysates at 50 µg
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 alpha antibody (ab226336)
Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for LAP2 alpha using ab226336 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB staining.
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