Anti-LAP2 alpha antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody (AB5162)

ab5162 at a dilution of 1/250 staining LAP 2 alpha in HeLa cells by immunofluorescence.

A = ab5162, 1/250

B = DNA

C = A and B merged

• WB

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Western blot - Anti-LAP2 alpha antibody (AB5162)

ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL). ab5162 at dilutions of 1/100 and 1/500 staining approximately 83 kDa LAP 2 alpha in HeLa cell extracts by Western blot (ECL).

All lanes:

Western blot - Anti-LAP2 alpha antibody (ab5162)

Predicted band size: 75 kDa

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• WB

CiteAb

Western blot - Anti-LAP2 alpha antibody (AB5162)

LAP2 alpha western blot using anti-LAP2 alpha antibody ab5162. Publication image and figure legend from Fahrenkrog, B., Martinelli, V., et al., 2016, PLoS One, PubMed 27031510.

ab5162 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab5162 please see the product overview.

Expression of Nup98 fusions perturbs the nuclear distribution of lamina-associated polypeptide 2α (LAP2α).HeLa cells were transiently transfected with GFP constructs and fixed and stained after 24 hours for immunofluorescence microscopy. (A) Lamin A/C (LA/C, red) concentrates at the nuclear envelope in HeLa control cells and in Nup98 expressing cells (green), while LAP2α (magenta) is found throughout the nucleoplasm. In HeLa cells expressing Nup98-HOXA9 (A) and Nup98-HHEX (B), LAP2α diminished from the nucleoplasm and aggregates at the nuclear periphery. Disruption of the homeodomain in HOXA9 (A) and HHEX (B) and the FG domain of Nup98 (A and B) prevent the relocation of the lamina proteins. DAPI was used to visualize DNA (merge). (B) Fluorescence intensity of LAP2α staining was determined along the axis shown as line in the fluorescence images and plotted as a graph. (D) Quantification of cells with altered LA/C and LAP2α distribution. About 400 cells were analyzed for each sample. (E) Western blot analysis of the expression levels of LA/C, LAP2α, and LB1.

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