Anti-LAP2 alpha antibody [EPR28581-87] KO Tested (ab317028)

Knockout Tested Rabbit Recombinant Monoclonal LAP2 alpha antibody. Suitable for WB, Flow Cyt (Intra), IHC-P, ICC/IF, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Western blot - Anti-LAP2 alpha antibody [EPR28581-87] (AB317028), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	Flow Cyt (Intra)	IHC-P	ICC/IF	IP
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Tested	Tested	Expected
Rat	Tested	Expected	Tested	Expected	Expected
Recombinant fragment - Human	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/5000	Notes -
Species Mouse	Dilution info 1/5000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/500	Notes -
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Target data

See full target information TMPO

Function

May be involved in the structural organization of the nucleus and in the post-mitotic nuclear assembly. Plays an important role, together with LMNA, in the nuclear anchorage of RB1. TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.

Alternative names

LAP2, TMPO, Thymopoietin isoform alpha, Thymopoietin-related peptide isoform alpha, TP alpha, TPRP isoform alpha

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal LAP2 alpha antibody. Suitable for WB, Flow Cyt (Intra), IHC-P, ICC/IF, IP and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR28581-87
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Lamina-associated polypeptide 2 alpha (LAP2 alpha) also known as LAP2A is part of the LAP2 protein family. It has a molecular mass of approximately 76 kDa. This protein connects with the nuclear lamina and is prominently expressed in the nucleus of most cell types. LAP2 alpha interacts with chromatin and its mechanical role involves regulating interactions between chromatin and nuclear structures.

Biological function summary

LAP2 alpha contributes to processes like nuclear stability and chromatin organization. It is involved in the formation of a complex with lamin A/C facilitating the association of this nuclear lamina protein. This interaction plays a significant role in dynamically organizing the nuclear architecture throughout the cell cycle influencing cell differentiation and proliferation.

Pathways

The interaction of LAP2 alpha with lamin A/C places it in the context of the nuclear envelope dynamics. Key pathways include the regulation of genome stability and DNA repair pathways. The interaction with lamin A/C influences how cells manage genome maintenance making LAP2 alpha important for cellular homeostasis.

Associated diseases and disorders

LAP2 alpha shows relevance to muscular dystrophy and progeria. LAP2 alpha’s interaction with lamin A/C is important because mutations in lamin A/C are known to cause Emery-Dreifuss muscular dystrophy. Additionally LAP2 alpha's role in nuclear architecture is significant in the context of Hutchinson-Gilford progeria syndrome where abnormalities in nuclear shape are evident. In these diseases LAP2 alpha's interaction or malfunction can lead to or exacerbate pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
This antibody does not cross-react with human LAP2 beta, LAP2 gamma and LAP2 zeta.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
All lanes: Western blot - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028) at 1/1000 dilution
Lane 1: His-tagged human LAP2 alpha fragment at 100 ng with 5% NFDM/TBST
Lane 2: His-tagged human LAP2 beta recombinant protein at 100 ng with 5% NFDM/TBST
Lane 3: His-tagged human LAP2 gamma recombinant protein at 100 ng with 5% NFDM/TBST
Lane 4: His-tagged human LAP2 zeta recombinant protein at 100 ng with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 57 kDa
Exposure time: 180s
Western blot - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
In Western blot, ab317028 was shown to bind specifically to LAP2 alpha. Target of interest was observed at 76 kDa in wild-type HEK-293 cell lysates (lane 1/4) with no signal observed at this size in TMPO knockout cell line (lane 2/5) (knockout cell line ab266774 / knockout cell lysate ab257747).
In lanes 1-3, the lysates were stored at -80℃ prior to Western Blotting. The bands beneath the target band (76 kDa) are likey to be degradation products. In lanes 4-13, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
The identity of the higher MW band at approximately 250 kDa (in lane 10) is unknown.
The lanes 1-2 were developed using a high sensitivity ECL substrate.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-2: 81 seconds, lanes 3-13: 180 seconds
All lanes: Western blot - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028) at 1/1000 dilution
Lane 1: Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lanes 2 and 5: TMPO knockout HEK-293 whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: HEL (erythroleukemia erythroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 4: Wild-type HEK-293 whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 6: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 7: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 8: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 9: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 10: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 11: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 12: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 13: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 76 kDa, 36 kDa
Western blot - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
The bands beneath the target band (76 kDa) are likely to be degraded target fragments.
All lanes: Western blot - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse spleen tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Rat testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Rat spleen tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 76 kDa
Exposure time: 180s
Flow Cytometry (Intracellular) - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling LAP2 alpha with ab317028 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized TMPO KO HEK293T (LAP2 alpha knock out human embryonic kidney epithelial cell, Left) / Parental HEK293T (Right) cells labelling LAP2 alpha with ab317028 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling LAP2 alpha with ab317028 at 1/500 (1.046 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing nuclear staining in NIH/3T3 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunocytochemistry/ Immunofluorescence - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TMPO KO HEK293T (TMPO knockout human embryonic kidney epithelial cell) (ab266774) cells labelling LAP2 alpha with ab317028 at 1/500 (1.046 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing nuclear staining in parental HEK293T cells (shown in green), no staining in TMPO KO HEK293T cells. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling LAP2 alpha with ab317028 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat spleen. The section was incubated with ab317028 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling LAP2 alpha with ab317028 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse spleen. The section was incubated with ab317028 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling LAP2 alpha with ab317028 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human gastric carcinoma (PMID: 22745766). The section was incubated with ab317028 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling LAP2 alpha with ab317028 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil. The section was incubated with ab317028 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunoprecipitation - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028)
LAP2 alpha was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab317028 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317028 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

Lane 2: ab317028 IP in HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab317028 in HEK-293 whole cell lysate.
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.
All lanes: Immunoprecipitation - Anti-LAP2 alpha antibody [EPR28581-87] (ab317028) at 1/30 dilution
All lanes: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with 5% NFDM/TBST
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s