AB185718

Anti-LAP2 antibody

Name: Anti-LAP2 antibody (ab185718) | Abcam
Brand: Abcam Limited
SKU: AB185718
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal LAP2B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications.

View Alternative Names

LAP2, TMPO, Thymopoietin-related peptide isoforms beta/gamma, TP beta/gamma, TPRP isoforms beta/gamma

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (AB185718)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (AB185718)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling LAP2 with ab185718 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (AB185718)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling LAP2 with ab185718 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (AB185718)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling LAP2 with ab185718 at 1/200. Magnification : 200x.

Immunocytochemistry/ Immunofluorescence - Anti-LAP2 antibody (AB185718)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LAP2 antibody (AB185718)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab185718. Blue DAPI for nuclear staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (AB185718)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling LAP2 with ab185718 at 1/200. Magnification : 400x.

Supplier Data

Western blot - Anti-LAP2 antibody (AB185718)

All lanes:

Western blot - Anti-LAP2 antibody (ab185718) at 1/500 dilution

Lane 1:

Extract from 22RV1 cell line

Lane 2:

Extract from K562 cell line

Lane 3:

Extract from HepG2 cell line

Lane 4:

Extract from 293T cell line

Lane 5:

Extract from SH-SY5Y cell line

Lane 6:

Extract from H460 cell line

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LAP2 protein also known as Lamin-Associated Polypeptide 2 plays a significant role in the structural organization of the nuclear envelope. This protein exists in various isoforms with LAP2β being among the most studied. LAP2 has a molecular mass ranging from 45 to 80 kDa depending on its isoform. LAP2 shows expression in nuclear membrane and endoplasmic reticulum and these locations enable it to participate in the anchorage of chromatin and nuclear lamina to the inner nuclear membrane.

Biological function summary

The LAP2 protein acts as a scaffold for several nuclear processes. It interacts with lamin proteins forming complexes that are vital for both nuclear assembly and structural stability. Through these interactions LAP2 not only assists in maintaining nuclear shape but also plays a role in DNA replication and gene expression regulation. By facilitating interactions between chromatin and the inner nuclear membrane LAP2 influences the spatial organization of genetic material within the nucleus.

Pathways

The LAP2 protein engages in the regulation of key cellular processes such as the cell cycle and apoptosis. In the context of the cell cycle LAP2 associates with nuclear lamins and other nuclear envelope components impacting the dynamic disassembly and reassembly of the nuclear envelope during mitosis. In apoptosis pathways LAP2 interacts with proteins like BAF (Barrier-to-Autointegration Factor) affecting chromatin condensation and DNA fragmentation phases.

LAP2 protein alterations show connections to cardiac and muscular pathologies. Mutations or dysregulation of LAP2 protein can contribute to Emery-Dreifuss muscular dystrophy a condition often linked with mutant nuclear envelope proteins including emerin and lamins. Additionally LAP2 has relevance in dilated cardiomyopathy where its interaction with lamin A/C becomes impaired leading to improper nuclear structural integrity and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with NAKAP95.. Thymopoietin (TP) and Thymopentin (TP5) may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.

See full target information TMPO

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer research communications 4:2621-2637 PubMed39248577

2024

Antiangiogenic Tyrosine Kinase Inhibitors have Differential Efficacy in Clear Cell Renal Cell Carcinoma in Bone.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Maksimovic,Nina C Boscolo,Ludovica La Posta,Sergio Barrios,Mohammad Jad Moussa,Emanuela Gentile,Pedro I Pesquera,Wenjiao Li,Jianfeng Chen,Javier A Gomez,Akshay Basi,Jared K Burks,Christopher Alvarez-Breckenridge,Jianjun Gao,Matthew T Campbell,Eleonora Dondossola

Nature communications 12:3176 PubMed34039995

2021

The SUN1-SPDYA interaction plays an essential role in meiosis prophase I.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Chen,Yan Wang,Juan Chen,Wu Zuo,Yong Fan,Sijia Huang,Yongmei Liu,Guangming Chen,Qing Li,Jinsong Li,Jian Wu,Qian Bian,Chenhui Huang,Ming Lei

Cancer cell international 20:294 PubMed32669970

2020

TMPO-AS1 promotes cell proliferation of thyroid cancer via sponging miR-498 to modulate TMPO.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Li,Yun Feng,Zhen Zhang,Xiaozhong Cao,Xiubo Lu

Cell 176:198-212.e15 PubMed30503211

2018

LAP2 Proteins Chaperone GLI1 Movement between the Lamina and Chromatin to Regulate Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Amar N Mirza,Siegen A McKellar,Nicole M Urman,Alexander S Brown,Tyler Hollmig,Sumaira Z Aasi,Anthony E Oro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LAP2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (AB185718)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (AB185718)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (AB185718)

Immunocytochemistry/ Immunofluorescence - Anti-LAP2 antibody (AB185718)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP2 antibody (AB185718)

Western blot - Anti-LAP2 antibody (AB185718)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise