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Rabbit Recombinant Monoclonal LAP3 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

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Images

Western blot - Anti-LAP3 antibody [EPR10330] (AB154809), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (AB154809), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-LAP3 antibody [EPR10330] (AB154809), expandable thumbnail
  • Western blot - Anti-LAP3 antibody [EPR10330] (AB154809), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (AB154809), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Not recommended
Tested
Rat
Predicted
Not recommended
Predicted
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Target data

Function

Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides. The presence of Zn(2+) ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme. For instance, in the presence of Mn(2+), it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates. Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status.

Alternative names

LAPEP, PEPS, LAP3, Cytosol aminopeptidase, Cysteinylglycine-S-conjugate dipeptidase, Leucine aminopeptidase 3, Leucyl aminopeptidase, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase, LAP-3

Recommended products

Rabbit Recombinant Monoclonal LAP3 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10330
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAP3 also known as leukotriene A4 hydrolase has a molecular weight of approximately 53 kDa. This enzyme functions as an aminopeptidase which is a type of enzyme that removes amino acids from the amino terminus of proteins and peptides. It is expressed in many tissues with significant levels found in the liver and kidney. LAP3 also shows activity in the cellular cytoplasm indicating it plays a role in cellular metabolic processes.

Biological function summary

LAP3 participates in protein maturation and degradation. It plays a role in processing peptides that are destined for further degradation or cellular signaling. The LAP3 protein is not known to be part of a larger complex but it works alongside other enzymes involved in peptide metabolism. This activity contributes to the regulation of intracellular peptide concentrations and supports cellular homeostasis.

Pathways

LAP3 is linked to important biological processes such as protein catabolism and the regulation of blood pressure. It interacts within the peptide processing pathways to influence the generation of bioactive peptides. LAP3 works in conjunction with other proteolytic enzymes such as aminopeptidases and endopeptidases which are essential for various cellular functions. Through these pathways LAP3 indirectly impacts the regulation of physiological processes.

Associated diseases and disorders

LAP3 is associated with hypertension and certain metabolic disorders. It influences conditions like high blood pressure through its role in generating peptides that can modulate vascular functions. LAP3 has connections with proteins involved in these conditions such as angiotensin-converting enzyme (ACE) which also plays a part in blood pressure regulation. Understanding the functions of LAP3 aids in delineating its potential as a therapeutic target for these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-LAP3 antibody [EPR10330] (ab154809), expandable thumbnail

    Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)

    Lanes 1-4: Merged signal (red and green). Green - ab154809 observed at 56 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab154809 Anti-LAP3 antibody [EPR10330] was shown to specifically react with LAP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line Human LAP3 knockout A549 cell line ab266986 (knockout cell lysate Human LAP3 knockout A549 cell lysate ab258023) was used. Wild-type and LAP3 knockout samples were subjected to SDS-PAGE. ab154809 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: LAP3 knockout A549 cell lysate at 20 µg

    Lane 2: Western blot - Human LAP3 knockout A549 cell line (Human LAP3 knockout A549 cell line ab266986)

    Lane 3: HepG2 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 56 kDa

    Observed band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling LAP3 with ab154809 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-LAP3 antibody [EPR10330] (ab154809), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-LAP3 antibody [EPR10330] (ab154809)

    Intracellular flow cytometric analysis of permeabilized 293T cells labeling LAP3 with ab154809 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).

  • Western blot - Anti-LAP3 antibody [EPR10330] (ab154809), expandable thumbnail

    Western blot - Anti-LAP3 antibody [EPR10330] (ab154809)

    All lanes: Western blot - Anti-LAP3 antibody [EPR10330] (ab154809) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: 293T lysate at 10 µg

    Lane 3: HepG2 lysate at 10 µg

    Lane 4: HeLa lysate at 10 µg

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

    Immunohistochemical analysis of paraffin-embedded ovarian human carcinoma tissue labeling LAP3 with ab154809 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

    Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154809 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154809 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

    Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154809 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] (ab154809)

    Immunohistochemical analysis of paraffin embedded Human colonic adenocarcinoma tissue using ab154809 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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