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AB249116

Anti-LAP3 antibody [EPR10330] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal LAP3 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

LAPEP, PEPS, LAP3, Cytosol aminopeptidase, Cysteinylglycine-S-conjugate dipeptidase, Leucine aminopeptidase 3, Leucyl aminopeptidase, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase, LAP-3

8 Images
Flow Cytometry (Intracellular) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)

This data was developed using ab154809, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells labeling LAP3 with ab154809 at 1/10 dilution (red) or a negative rabbit IgG (control) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)

This data was developed using ab154809, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded ovarian human carcinoma tissue labeling LAP3 with ab154809 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)

This data was developed using ab154809, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling LAP3 with ab154809 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)

This data was developed using ab154809, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)

This data was developed using ab154809, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human urinary bladder transitional carcinoma tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)

This data was developed using ab154809, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154809 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)
  • WB

Lab

Western blot - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)

This data was developed using the same antibody clone in a different buffer formulation (ab154809).

Lanes 1-4 : Merged signal (red and green). Green - ab154809 observed at 56 kDa. Red - loading control ab8245 observed at 36 kDa.

ab154809 Anti-LAP3 antibody [EPR10330] was shown to specifically react with LAP3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266986 (knockout cell lysate ab258023) was used. Wild-type and LAP3 knockout samples were subjected to SDS-PAGE. ab154809 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-LAP3 antibody [EPR10330] (<a href='/en-us/products/primary-antibodies/lap3-antibody-epr10330-ab154809'>ab154809</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

LAP3 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human LAP3 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-lap3-knockout-a549-cell-line-ab266986'>ab266986</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 56 kDa

Observed band size: 56 kDa

false

Western blot - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)
  • WB

Unknown

Western blot - Anti-LAP3 antibody [EPR10330] - BSA and Azide free (AB249116)

This data was developed using ab154809, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LAP3 antibody [EPR10330] (<a href='/en-us/products/primary-antibodies/lap3-antibody-epr10330-ab154809'>ab154809</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

293T lysate at 10 µg

Lane 3:

HepG2 lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10330

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249116 is the carrier-free version of ab154809.

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAP3 also known as leukotriene A4 hydrolase has a molecular weight of approximately 53 kDa. This enzyme functions as an aminopeptidase which is a type of enzyme that removes amino acids from the amino terminus of proteins and peptides. It is expressed in many tissues with significant levels found in the liver and kidney. LAP3 also shows activity in the cellular cytoplasm indicating it plays a role in cellular metabolic processes.
Biological function summary

LAP3 participates in protein maturation and degradation. It plays a role in processing peptides that are destined for further degradation or cellular signaling. The LAP3 protein is not known to be part of a larger complex but it works alongside other enzymes involved in peptide metabolism. This activity contributes to the regulation of intracellular peptide concentrations and supports cellular homeostasis.

Pathways

LAP3 is linked to important biological processes such as protein catabolism and the regulation of blood pressure. It interacts within the peptide processing pathways to influence the generation of bioactive peptides. LAP3 works in conjunction with other proteolytic enzymes such as aminopeptidases and endopeptidases which are essential for various cellular functions. Through these pathways LAP3 indirectly impacts the regulation of physiological processes.

LAP3 is associated with hypertension and certain metabolic disorders. It influences conditions like high blood pressure through its role in generating peptides that can modulate vascular functions. LAP3 has connections with proteins involved in these conditions such as angiotensin-converting enzyme (ACE) which also plays a part in blood pressure regulation. Understanding the functions of LAP3 aids in delineating its potential as a therapeutic target for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides. The presence of Zn(2+) ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme. For instance, in the presence of Mn(2+), it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates. Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status.
See full target information LAP3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze
View all publications
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