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AB136072

Anti-LARG antibody

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(2 Publications)

Rabbit Polyclonal LARG antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ARHGEF12.

View Alternative Names

KIAA0382, LARG, ARHGEF12, Rho guanine nucleotide exchange factor 12, Leukemia-associated RhoGEF

1 Images
Western blot - Anti-LARG antibody (AB136072)
  • WB

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Western blot - Anti-LARG antibody (AB136072)

All lanes:

Western blot - Anti-LARG antibody (ab136072) at 1/500 dilution

All lanes:

HUVEC cell lysate at 30 µg

Predicted band size: 173 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ARHGEF12. The exact immunogen used to generate this antibody is proprietary information.

Q9NZN5

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LARG also known as Leukemia Associated Rho Guanine Nucleotide Exchange Factor is a RhoGEF involved in the regulation of Rho GTPases. It functions mechanically by facilitating the exchange of GDP for GTP on Rho proteins thereby activating them. LARG has a molecular mass of approximately 173 kDa. It expresses in various tissues including the brain heart and kidney highlighting its potential involvement in diverse biological processes.
Biological function summary

LARG influences cytoskeletal dynamics and cell mobility. It associates with G-protein coupled receptor signaling complexes impacting cellular responses to external stimuli. This protein also has important roles in the regulation of cell adhesion migration and proliferation. Through interactions with other proteins such as PDZ-RhoGEF and p115-RhoGEF LARG influences the organization of the actin cytoskeleton and is involved in maintaining tissue architecture.

Pathways

LARG integrates into the RhoA signaling pathway and is an important player in the regulation of cellular structure and motility. It interacts with proteins involved in the Rho GTPase cycle such as RhoA and RhoC and modulates actin cytoskeletal arrangement. LARG also participates in the Wnt signaling pathway influencing gene expression and cell behavior. Through these interactions LARG contributes to regulating processes like cell morphology and division.

LARG has connections to certain cancers and cardiovascular disorders. It interacts with proteins such as Gα13 and RhoA which have implications in the progression of these diseases. In cancer alterations in LARG expression or function may lead to enhanced cell proliferation and metastasis. In cardiovascular disorders LARG-related pathways might affect cell contraction and architecture in vascular tissues contributing to pathological states. Understanding LARG's role in these contexts could help develop targeted therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
See full target information ARHGEF12
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 3: PubMed30385725

2018

Arhgef12 drives IL17A-induced airway contractility and airway hyperresponsiveness in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie Fong,Austin Hsu,Esther Wu,Agnieszka P Looney,Previn Ganesan,Xin Ren,Dean Sheppard,Sarah A Wicher,Michael A Thompson,Rodney D Britt,Y S Prakash,Mallar Bhattacharya

Genes & development 32:781-793 PubMed29891559

2018

A RhoA-YAP-c-Myc signaling axis promotes the development of polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Cai,Xuewen Song,Wei Wang,Terry Watnick,York Pei,Feng Qian,Duojia Pan
View all publications
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