AB86095

Anti-LARG antibody

Name: Anti-LARG antibody (ab86095) | Abcam
Brand: Abcam Limited
SKU: AB86095

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(1 Publication)

Rabbit Polyclonal LARG antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARHGEF12 aa 1350-1400.

View Alternative Names

KIAA0382, LARG, ARHGEF12, Rho guanine nucleotide exchange factor 12, Leukemia-associated RhoGEF

2 Images

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Immunoprecipitation - Anti-LARG antibody (AB86095)

Detection of LARG by Western Blot of Immunprecipitate.
ab86095 at 1µg/ml staining LARG in HeLa whole cell lysate immunoprecipitated using ab86095 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-LARG antibody (ab86095)

Predicted band size: 173 kDa

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Unknown

Western blot - Anti-LARG antibody (AB86095)

All lanes:

Western blot - Anti-LARG antibody (ab86095) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 173 kDa

Observed band size: 173 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ARHGEF12 aa 1350-1400. The exact immunogen used to generate this antibody is proprietary information.

Q9NZN5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab86095 was affinity purified using an epitope specific to LARG immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LARG also known as Leukemia Associated Rho Guanine Nucleotide Exchange Factor is a RhoGEF involved in the regulation of Rho GTPases. It functions mechanically by facilitating the exchange of GDP for GTP on Rho proteins thereby activating them. LARG has a molecular mass of approximately 173 kDa. It expresses in various tissues including the brain heart and kidney highlighting its potential involvement in diverse biological processes.

Biological function summary

LARG influences cytoskeletal dynamics and cell mobility. It associates with G-protein coupled receptor signaling complexes impacting cellular responses to external stimuli. This protein also has important roles in the regulation of cell adhesion migration and proliferation. Through interactions with other proteins such as PDZ-RhoGEF and p115-RhoGEF LARG influences the organization of the actin cytoskeleton and is involved in maintaining tissue architecture.

Pathways

LARG integrates into the RhoA signaling pathway and is an important player in the regulation of cellular structure and motility. It interacts with proteins involved in the Rho GTPase cycle such as RhoA and RhoC and modulates actin cytoskeletal arrangement. LARG also participates in the Wnt signaling pathway influencing gene expression and cell behavior. Through these interactions LARG contributes to regulating processes like cell morphology and division.

LARG has connections to certain cancers and cardiovascular disorders. It interacts with proteins such as Gα13 and RhoA which have implications in the progression of these diseases. In cancer alterations in LARG expression or function may lead to enhanced cell proliferation and metastasis. In cardiovascular disorders LARG-related pathways might affect cell contraction and architecture in vascular tissues contributing to pathological states. Understanding LARG's role in these contexts could help develop targeted therapies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.

See full target information ARHGEF12

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 25:3528-40 PubMed25143398

2014

TGF-β regulates LARG and GEF-H1 during EMT to affect stiffening response to force and cell invasion.

Applications

Species

Unspecified reactive species

Lukas D Osborne,George Z Li,Tam How,E Tim O'Brien,Gerard C Blobe,Richard Superfine,Karthikeyan Mythreye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LARG antibody

Immunoprecipitation - Anti-LARG antibody (AB86095)

Western blot - Anti-LARG antibody (AB86095)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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