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AB243183

Anti-Large subunit of NADH-dependent glutamate synthase antibody

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(1 Publication)

Rabbit Polyclonal Large subunit of NADH-dependent glutamate synthase antibody. Suitable for ELISA, WB and reacts with Synechocystis sp samples. Cited in 1 publication.
1 Images
Western blot - Anti-Large subunit of NADH-dependent glutamate synthase antibody (AB243183)
  • WB

Supplier Data

Western blot - Anti-Large subunit of NADH-dependent glutamate synthase antibody (AB243183)

All lanes:

Western blot - Anti-Large subunit of NADH-dependent glutamate synthase antibody (ab243183) at 4 µg/mL

All lanes:

Soluble fraction of Synechocystis sp. PCC6803 extract

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Synechocystis sp

Applications

WB, ELISA

applications

Specificity

Reacts with cyanobacteria glutamate synthase, GltB and GltD proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Synechocystis sp": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The large subunit of NADH-dependent glutamate synthase also known as GltB participates in the conversion of glutamine and 2-oxoglutarate to glutamate. This enzyme plays a role in nitrogen assimilation and has a molecular mass of approximately 150 kDa. The large subunit of NADH-dependent glutamate synthase is mostly found in the chloroplasts of plants and cyanobacteria though it can also appear in certain bacteria where it aids in essential metabolic activities.
Biological function summary

The large subunit engages in forming glutamate which is a fundamental amino acid in many organisms. It acts as part of a protein complex where it joins with a small subunit (GltD) to complete its enzymatic function. This protein complex ensures the efficient transfer of nitrogen within the organism contributing to amino acid biosynthesis and cellular growth functions.

Pathways

The large subunit of NADH-dependent glutamate synthase plays an essential part in the nitrogen cycle and glutamate metabolism. The enzyme interacts within the pathway alongside proteins like glutamine synthetase facilitating the conversion of ammonia to amino acids. This pathway involvement highlights its roles in metabolite interconversion and energy cycling in cells.

Disruptions in the function of this large subunit of glutamate synthase could lead to issues in nitrogen metabolism such as metabolic syndromes in plants or cyanobacteria. Proteins like glutamate decarboxylase link to this target under pathological conditions where imbalanced glutamate levels could contribute to neurological disorders. Understanding these interactions helps researchers target pathways in agricultural or microbial settings aiming to improve growth efficiency and health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Large subunit of NADH-dependent glutamate synthase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed32455738

2020

Interstitial Leydig Cell Tumorigenesis-Leptin and Adiponectin Signaling in Relation to Aromatase Expression in the Human Testis.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Duliban,Ewelina Gorowska-Wojtowicz,Waclaw Tworzydlo,Agnieszka Rak,Malgorzata Brzoskwinia,Izabella Krakowska,Jan K Wolski,Malgorzata Kotula-Balak,Bartosz J Płachno,Barbara Bilinska
View all publications
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Product promise

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