Rabbit Polyclonal LARP1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human LARP1 aa 250-350.
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- Stem cell reports 18:254-2682022The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarino Caruso,Sébastien Meurant,Damien Detraux,Amandine Mathieu,Manon Gilson,Marc Dieu,Antoine Fattaccioli,Catherine Demazy,Mustapha Najimi,Etienne Sokal,Thierry Arnould,Catherine Verfaillie,Denis L J Lafontaine,Patricia RenardPubMed 36563686
- The Journal of biological chemistry 298:1022772022The amino acid sensor GCN2 suppresses terminal oligopyrimidine (TOP) mRNA translation via La-related protein 1 (LARP1).Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeenat Farooq,Fedho Kusuma,Phillip Burke,Catherine R Dufour,Duckgue Lee,Negar Tabatabaei,Phoenix Toboz,Ernest Radovani,Jack F Greenblatt,Jalees Rehman,Jacob Class,Arkady Khoutorsky,Bruno D Fonseca,Justin M Richner,Eloi Mercier,Guillaume Bourque,Vincent Giguère,Arvind R Subramaniam,Jaeseok Han,Soroush TahmasebiPubMed 35863436
- World journal of surgical oncology 20:1082022Circ_0047921 acts as the sponge of miR-1287-5p to stimulate lung cancer progression by regulating proliferation, migration, invasion, and glycolysis of lung cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuehua Xiao,Shequn Gu,Wenxiu Yao,Ling Qin,Jihui LuoPubMed 35365169
- Bioengineered 13:7181-71962022Circular RNA coiled-coil domain containing 66 regulates malignant development of papillary thyroid carcinoma by upregulating La ribonucleoprotein 1 the sponge effect on miR-129-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeipei Li,Junhui Chen,Jun Zou,Wei Zhu,Yan Zang,Hongwu LiPubMed 35264065
- Breast cancer research and treatment 189:607-6192021Long non-coding RNA ARHGAP5-AS1 inhibits migration of breast cancer cell via stabilizing SMAD7 protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen-Long Wang,Jing-Chi Li,Ci-Xiang Zhou,Cheng-Ning Ma,Di-Fei Wang,Lu-Lu Wo,Ming He,Qianqian Yin,Jian-Rong He,Qian ZhaoPubMed 34370213
- Nucleic acids research 49:6722-67382021Upregulation of RNA cap methyltransferase RNMT drives ribosome biogenesis during T cell activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlison Galloway,Aneesa Kaskar,Dimitrinka Ditsova,Abdelmadjid Atrih,Harunori Yoshikawa,Carolina Gomez-Moreira,Olga Suska,Marcin Warminski,Renata Grzela,Angus I Lamond,Edward Darzynkiewicz,Jacek Jemielity,Victoria H CowlingPubMed 34125914
- Frontiers in cell and developmental biology 8:6257302021Long Non-coding RNA Promotes Ovarian Cancer by Regulating the miR-144-5p/LARP1 Axis as a Competing Endogenous RNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinxin Chen,Xiaocen Li,Lu Yang,Jingru ZhangPubMed 33614632
- Nucleic acids research :2021mTORC1 promotes TOP mRNA translation through site-specific phosphorylation of LARP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian-Jun Jia et. al.PubMed 33398329
- RNA biology 18:237-2472020LARP1 isoform expression in human cancer cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHagen Schwenzer,Mai Abdel Mouti,Pia Neubert,Josephine Morris,Joanne Stockton,Sarah Bonham,Martin Fellermeyer,James Chettle,Roman Fischer,Andrew D Beggs,Sarah P BlagdenPubMed 32286153
- The Journal of cell biology 219:2020CDK1 couples proliferation with protein synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Haneke,Johanna Schott,Doris Lindner,Anne Kruse Hollensen,Christian Kroun Damgaard,Cyril Mongis,Michael Knop,Wilhelm Palm,Alessia Ruggieri,Georg StoecklinPubMed 32040547
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human LARP1 aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PKG0
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla | Dilution info - | Notes - |
Associated Products
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Target data
Function
RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability (PubMed:20430826, PubMed:23711370, PubMed:24532714, PubMed:25940091, PubMed:28650797, PubMed:28673543, PubMed:29244122). Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery (PubMed:23711370, PubMed:25940091, PubMed:26206669, PubMed:28379136, PubMed:28650797, PubMed:29244122). The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1 (PubMed:25940091, PubMed:28379136, PubMed:28650797, PubMed:29244122). When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA (PubMed:25940091, PubMed:28650797). Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif (PubMed:28379136). Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes (PubMed:20430826, PubMed:24532714, PubMed:25940091, PubMed:28673543). Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis (PubMed:23711370, PubMed:28650797). Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation (PubMed:20430826, PubMed:24532714). Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis (PubMed:20430826, PubMed:23711370, PubMed:25940091, PubMed:28673543). (Microbial infection) Positively regulates the replication of dengue virus (DENV).
Alternative names
KIAA0731, LARP, LARP1, La-related protein 1, La ribonucleoprotein domain family member 1
Recommended products
Rabbit Polyclonal LARP1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human LARP1 aa 250-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human LARP1 aa 250-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q6PKG0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LARP1 or La-related protein 1 plays an important role in mRNA metabolism by regulating translation. This protein has a molecular weight of approximately 150 kDa and is expressed ubiquitously in various human tissues. LARP1 contains conserved domains that enable it to interact with RNA making it an important player in gene expression regulation at the post-transcriptional level. LARP1 is also involved in regulating the stability and translation of target mRNAs especially those with a specific 5' terminal oligopyrimidine (TOP) motif.
- Biological function summary
LARP1 acts as an important regulator in the translation of mRNA particularly within the mTOR (mechanistic target of rapamycin) signaling pathway. LARP1 functions as part of a molecular complex that includes mTOR and other translation-associated proteins. This involvement in the mTOR pathway links LARP1 to protein synthesis control which is important for cell growth proliferation and survival. The interaction between LARP1 and mTORC1 (mTOR complex 1) enhances the translational efficiency of certain mRNAs influencing cellular responses to nutritional and growth signals.
- Pathways
LARP1 interacts significantly with the mTORC1 signaling pathway which is important in cell growth and metabolism regulation. Its activity connects to proteins like eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) that are important for controlling protein synthesis. The function of LARP1 in these pathways helps maintain cellular homeostasis especially under varying environmental conditions by modulating the translation of mRNAs that encode for ribosomal proteins and other components of the translational machinery.
- Associated diseases and disorders
LARP1 has been implicated in cancer and metabolic diseases due to its role in mTORC1 signaling. Dysregulation of LARP1 expression or function can lead to aberrant cell growth and proliferation which is common in cancers. LARP1's association with mTORC1 and 4EBP1 highlights its involvement in metabolic disorders such as insulin resistance where altered mRNA translation can disrupt normal metabolic processes. Understanding LARP1's role in these conditions may provide insights into therapeutic targeting for cancer and metabolic disease interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunoprecipitation - Anti-LARP1 antibody (ab86359)
Detection of human LARP1 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: Rabbit polyclonal to LARP1 (ab86359) at 3 μg/mg lysate
Lane 2: Rabbit polyclonal to LARP1 (Anti-LARP1 antibody ab245635) at 3 μg/mg lysate
Lane 3: Control IgG
Detection: Chemiluminescence with an exposure time of 3 seconds. Anti-FTSJD2 antibody ab70386 was used at 0.4 ug/mL for Western blot.
All lanes: Immunoprecipitation - Anti-LARP1 antibody (ab86359)
Predicted band size: 124 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LARP1 antibody (ab86359)
ab86359 staining LARP1 in human colon carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde- fixed, paraffin embedded tissue sections). Samples were incubated with primary antibody at 1:200 dilution (1μg/ml).
Western blot - Anti-LARP1 antibody (ab86359)
Detection: Chemiluminescence with an exposure time of 3 seconds
All lanes: Western blot - Anti-LARP1 antibody (ab86359) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 15 µg
Lane 2: HEK293T whole cell lysate at 15 µg
Lane 3: Jurkat whole cell lysate at 15 µg
Predicted band size: 124 kDa
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