Anti-LARP1 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LARP1 antibody (AB86359)

ab86359 staining LARP1 in human colon carcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde- fixed, paraffin embedded tissue sections). Samples were incubated with primary antibody at 1 : 200 dilution (1μg/ml).

• IP

Supplier Data

Immunoprecipitation - Anti-LARP1 antibody (AB86359)

Detection of human LARP1 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

Lane 1 : Rabbit polyclonal to LARP1 (ab86359) at 3 μg/mg lysate

Lane 2 : Rabbit polyclonal to LARP1 (ab245635) at 3 μg/mg lysate

Lane 3 : Control IgG

Detection : Chemiluminescence with an exposure time of 3 seconds. ab70386 was used at 0.4 ug/mL for Western blot.

All lanes:

Immunoprecipitation - Anti-LARP1 antibody (ab86359)

Predicted band size: 124 kDa

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• WB

Unknown

Western blot - Anti-LARP1 antibody (AB86359)

Detection : Chemiluminescence with an exposure time of 3 seconds

All lanes:

Western blot - Anti-LARP1 antibody (ab86359) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 15 µg

Lane 2:

HEK293T whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 15 µg

Predicted band size: 124 kDa

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• WB

Lab

Western blot - Anti-LARP1 antibody (AB86359)

Western blot : Anti-LARP1 antibody ab86359 staining at 0.1 µg/mL, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 150 kDa in Wild-type A549 cell lysates with no signal observed at this size in LARP1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc BSA in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-LARP1 antibody (ab86359) at 0.1 µg/mL

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

LARP1 knockout A549 at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 124 kDa

Observed band size: 150 kDa,50 kDa

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