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AB317575

Anti-LARP1 antibody [EPR28797-92]

  • 20ul selling size
  • Recombinant
  • RabMAb
  • KO Validated
  • What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal LARP1 antibody. Suitable for WB, Dot and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication.

View Alternative Names

KIAA0731, LARP, LARP1, La-related protein 1, La ribonucleoprotein domain family member 1

3 Images
Western blot - Anti-LARP1 antibody [EPR28797-92] (AB317575)
  • WB

Lab

Western blot - Anti-LARP1 antibody [EPR28797-92] (AB317575)

Western blot : Anti-LARP1 antibody [EPR28797-92] ab317575 staining at 1/10000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 150 kDa in Wild-type A549 cell lysates with no signal observed at this size in LARP1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 100pc LICOR in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-LARP1 antibody [EPR28797-92] (ab317575) at 1/10000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

LARP1 knockout A549 at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 124 kDa

Observed band size: 150 kDa,50 kDa

false

Western blot - Anti-LARP1 antibody [EPR28797-92] (AB317575)
  • WB

Supplier Data

Western blot - Anti-LARP1 antibody [EPR28797-92] (AB317575)

The identity of the higher MW band at approximately 250 kDa is unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LARP1 antibody [EPR28797-92] (ab317575) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

HeLa transfected with siRNA specifically targeting LARP1 whole cell lysate at 20 µg with NFDM/TBST

Lane 3:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 4:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 5:

HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 150 kDa,124 kDa

false

Exposure time: 15s

Dot Blot - Anti-LARP1 antibody [EPR28797-92] (AB317575)
  • Dot

Supplier Data

Dot Blot - Anti-LARP1 antibody [EPR28797-92] (AB317575)

Dot blot analysis of LARP1 using ab317575 at 1 : 1000 (0.519 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human LARP1B.

All lanes:

Dot Blot - Anti-LARP1 antibody [EPR28797-92] (ab317575) at 1/1000 dilution

Lane 1:

His-tagged human LARP1 fragment

Lane 2:

His-tagged human LARP1B fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

  • Carrier free

    Anti-LARP1 antibody [EPR28797-92] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28797-92

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Not suitable for use with tissue lysates in WB. A predominant non-specific band at ~100KDa is observed.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LARP1 or La-related protein 1 plays an important role in mRNA metabolism by regulating translation. This protein has a molecular weight of approximately 150 kDa and is expressed ubiquitously in various human tissues. LARP1 contains conserved domains that enable it to interact with RNA making it an important player in gene expression regulation at the post-transcriptional level. LARP1 is also involved in regulating the stability and translation of target mRNAs especially those with a specific 5' terminal oligopyrimidine (TOP) motif.
Biological function summary

LARP1 acts as an important regulator in the translation of mRNA particularly within the mTOR (mechanistic target of rapamycin) signaling pathway. LARP1 functions as part of a molecular complex that includes mTOR and other translation-associated proteins. This involvement in the mTOR pathway links LARP1 to protein synthesis control which is important for cell growth proliferation and survival. The interaction between LARP1 and mTORC1 (mTOR complex 1) enhances the translational efficiency of certain mRNAs influencing cellular responses to nutritional and growth signals.

Pathways

LARP1 interacts significantly with the mTORC1 signaling pathway which is important in cell growth and metabolism regulation. Its activity connects to proteins like eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) that are important for controlling protein synthesis. The function of LARP1 in these pathways helps maintain cellular homeostasis especially under varying environmental conditions by modulating the translation of mRNAs that encode for ribosomal proteins and other components of the translational machinery.

LARP1 has been implicated in cancer and metabolic diseases due to its role in mTORC1 signaling. Dysregulation of LARP1 expression or function can lead to aberrant cell growth and proliferation which is common in cancers. LARP1's association with mTORC1 and 4EBP1 highlights its involvement in metabolic disorders such as insulin resistance where altered mRNA translation can disrupt normal metabolic processes. Understanding LARP1's role in these conditions may provide insights into therapeutic targeting for cancer and metabolic disease interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability (PubMed : 20430826, PubMed : 23711370, PubMed : 24532714, PubMed : 25940091, PubMed : 28650797, PubMed : 28673543, PubMed : 29244122). Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery (PubMed : 23711370, PubMed : 25940091, PubMed : 26206669, PubMed : 28379136, PubMed : 28650797, PubMed : 29244122). The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1 (PubMed : 25940091, PubMed : 28379136, PubMed : 28650797, PubMed : 29244122). When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA (PubMed : 25940091, PubMed : 28650797). Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif (PubMed : 28379136). Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes (PubMed : 20430826, PubMed : 24532714, PubMed : 25940091, PubMed : 28673543). Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis (PubMed : 23711370, PubMed : 28650797). Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation (PubMed : 20430826, PubMed : 24532714). Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis (PubMed : 20430826, PubMed : 23711370, PubMed : 25940091, PubMed : 28673543).. (Microbial infection) Positively regulates the replication of dengue virus (DENV).
See full target information LARP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Kaohsiung journal of medical sciences 41:e12934 PubMed39786317

2025

Telocinobufagin suppresses malignant metastasis of undifferentiated thyroid carcinoma via modulation of the LARP1-mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Zhi Qiang,Shi-Zhi Fang
View all publications
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Product promise

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