Anti-LARP1 antibody [EPR28797-92] - BSA and Azide free

• WB

Lab

Western blot - Anti-LARP1 antibody [EPR28797-92] - BSA and Azide free (AB317576)

This data was developed using ab317575, the same antibody clone in a different buffer formulation.

Western blot : Anti-LARP1 antibody [EPR28797-92] ab317575 staining at 1/10000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 150 kDa in Wild-type A549 cell lysates with no signal observed at this size in LARP1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 100pc LICOR in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-LARP1 antibody [EPR28797-92] (<a href='/en-us/products/primary-antibodies/larp1-antibody-epr28797-92-ab317575'>ab317575</a>) at 1/10000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

LARP1 knockout A549 at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 124 kDa

Observed band size: 150 kDa,50 kDa

false

• WB

Supplier Data

Western blot - Anti-LARP1 antibody [EPR28797-92] - BSA and Azide free (AB317576)

This data was developed using ab317575, the same antibody clone in a different buffer formulation.

The identity of the higher MW band at approximately 250 kDa is unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-LARP1 antibody [EPR28797-92] (<a href='/en-us/products/primary-antibodies/larp1-antibody-epr28797-92-ab317575'>ab317575</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

HeLa transfected with siRNA specifically targeting LARP1 whole cell lysate at 20 µg with NFDM/TBST

Lane 3:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 4:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 5:

HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 150 kDa,124 kDa

false

Exposure time: 15s

• Dot

Supplier Data

Dot Blot - Anti-LARP1 antibody [EPR28797-92] - BSA and Azide free (AB317576)

This data was developed using ab317575, the same antibody clone in a different buffer formulation.

Dot blot analysis of LARP1 using ab317575 at 1 : 1000 (0.519 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human LARP1B.

All lanes:

Dot Blot - Anti-LARP1 antibody [EPR28797-92] (<a href='/en-us/products/primary-antibodies/larp1-antibody-epr28797-92-ab317575'>ab317575</a>) at 1/1000 dilution

Lane 1:

His-tagged human LARP1 fragment

Lane 2:

His-tagged human LARP1B fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s