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AB241489

Anti-LARP4 antibody

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(4 Publications)

Rabbit Polyclonal LARP4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human LARP4 aa 650 to C-terminus.

View Alternative Names

PP13296, LARP4, La-related protein 4, La ribonucleoprotein domain family member 4

2 Images
Immunoprecipitation - Anti-LARP4 antibody (AB241489)
  • IP

Supplier Data

Immunoprecipitation - Anti-LARP4 antibody (AB241489)

LARP4 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP; 20% of IP loaded) using ab241489 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab241489 at 1 μg/ml.

Lane 1 : ab241489 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence withan exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-LARP4 antibody (ab241489)

Predicted band size: 81 kDa

false

Western blot - Anti-LARP4 antibody (AB241489)
  • WB

Supplier Data

Western blot - Anti-LARP4 antibody (AB241489)

All lanes:

Western blot - Anti-LARP4 antibody (ab241489) at 0.4 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 81 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human LARP4 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q71RC2

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Primary Antibodies

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Primary Antibodies

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Anti-Biotin antibody

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to LARP4 immobilized on solid support
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LARP4 also known as La-related protein 4 plays a role in RNA biology. LARP4 is a cytoplasmic protein with a mass of approximately 80 kDa. It is expressed in various tissues with notable presence in liver kidney and muscle tissues. LARP4 interacts with specific RNA motifs influencing RNA stability and translation. This protein associates with poly(A) binding proteins highlighting its role in RNA metabolism.
Biological function summary

LARP4 modulates RNA stability and translation efficiency. It associates with the poly(A) ribonucleoprotein complex where it influences mRNA turnover and translation rates. Through these interactions LARP4 supports proper protein synthesis aligning with cellular needs. In addition to impacting RNA stability LARP4 interacts with other proteins involved in ribosome recruitment and translation initiation further cementing its role in post-transcriptional regulation.

Pathways

LARP4 integrates into pathways affecting RNA processing and protein synthesis. It links closely with the mTOR signaling pathway which is important for cell growth and protein synthesis regulation. Within this pathway LARP4 interacts with proteins like PABP highlighting its contribution to mRNA translation control. Furthermore the interplay with mTOR signaling suggests a broader effect on cellular metabolism beyond RNA processing.

LARP4 has connections to cancer and muscular dystrophy. Its role in RNA stability links it to oncogenic processes where dysregulation might contribute to tumorigenesis. In some cancers aberrant LARP4 expression associates with increased tumor growth potentially through altered mRNA turnover. Additionally links between LARP4 and muscle protein synthesis issues connect it to muscular dystrophy pathogenesis wherein its interaction with dysregulated proteins impacts muscle integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA binding protein that binds to the poly-A tract of mRNA molecules (PubMed : 21098120). Associates with the 40S ribosomal subunit and with polysomes (PubMed : 21098120). Plays a role in the regulation of mRNA translation (PubMed : 21098120). Plays a role in the regulation of cell morphology and cytoskeletal organization (PubMed : 21834987, PubMed : 27615744).
See full target information LARP4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109288 PubMed38532886

2024

The RNA binding proteins LARP4A and LARP4B promote sarcoma and carcinoma growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer C Coleman,Luke Tattersall,Val Yianni,Laura Knight,Hongqiang Yu,Sadie R Hallett,Philip Johnson,Ana J Caetano,Charlie Cosstick,Anne J Ridley,Alison Gartland,Maria R Conte,Agamemnon E Grigoriadis

International journal of molecular sciences 24: PubMed37298552

2023

Endogenous TOM20 Proximity Labeling: A Swiss-Knife for the Study of Mitochondrial Proteins in Human Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sébastien Meurant,Lorris Mauclet,Marc Dieu,Thierry Arnould,Sven Eyckerman,Patricia Renard

Cancer cell international 20:5 PubMed31911757

2020

Up-regulation of circ_LARP4 suppresses cell proliferation and migration in ovarian cancer by regulating miR-513b-5p/LARP4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wumei Lin,Haiyan Ye,Keli You,Le Chen

Neurotherapeutics : the journal of the American So : PubMed31898284

2020

CCR1 Activation Promotes Neuroinflammation Through CCR1/TPR1/ERK1/2 Signaling Pathway After Intracerebral Hemorrhage in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Yan,Gang Zuo,Prativa Sherchan,Lei Huang,Umut Ocak,Weilin Xu,Zachary D Travis,Wenna Wang,John H Zhang,Jiping Tang
View all publications
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Product promise

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