Rabbit Polyclonal LARP4B antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LARP4B aa 600-650.
View Alternative Names
KIAA0217, LARP5, LARP4B, La-related protein 4B, La ribonucleoprotein domain family member 4B, La ribonucleoprotein domain family member 5, La-related protein 5
- WB
Supplier Data
Western blot - Anti-LARP4B antibody (AB197085)
Lysates prepared using NETN lysis buffer.
All lanes:
Western blot - Anti-LARP4B antibody (ab197085) at 0.1 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg
Lane 2:
293T whole cell lysate at 50 µg
Lane 3:
Jurkat whole cell lysate at 50 µg
Lane 4:
TCMK-1 whole cell lysate at 50 µg
Lane 5:
NIH3T3 whole cell lysate at 50 µg
Predicted band size: 81 kDa
true
Exposure time: 3min
- IP
Supplier Data
Immunoprecipitation - Anti-LARP4B antibody (AB197085)
Detection of LARP4B in Immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197085 at 6 μg/mg lysate for IP (Lane
- and at 0.1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 10 seconds.
All lanes:
Immunoprecipitation - Anti-LARP4B antibody (ab197085)
Predicted band size: 81 kDa
false
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LARP4B plays a role in regulating mRNA stability and translation. It often cooperates with mRNA decay and translation initiation factors. LARP4B does not form a stable complex but associates transiently with other RNA-binding proteins to perform its function. Its ability to bind mRNA suggests involvement in gene expression post-transcriptionally impacting various cellular processes.
Pathways
LARP4B is implicated in the mTOR signaling pathway and the RNA processing pathway. The mTOR pathway is essential for cell growth and proliferation linking nutrient availability to these processes. LARP4B works in tandem with other proteins like LARP1 and EIF4E to modulate mRNA stability indirectly influencing cell growth through translation regulation.
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Publications (2)
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Cell proliferation 53:e12823 PubMed32515533
2020
Applications
Unspecified application
Species
Unspecified reactive species
Molecular medicine reports 20:951-958 PubMed31173237
2019
Applications
Unspecified application
Species
Unspecified reactive species
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