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AB236954

Anti-LAS1L antibody

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(1 Publication)

Rabbit Polyclonal LAS1L antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human LAS1L aa 450-600.

View Alternative Names

MSTP060, LAS1L, Ribosomal biogenesis protein LAS1L, Endoribonuclease LAS1L, Protein LAS1 homolog

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-LAS1L antibody (AB236954)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAS1L antibody (AB236954)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for LAS1L (green) using ab236954 at 1/266 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated goat anti-rabbit IgG (H+L). Counterstained with DAPI (blue).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with primary antibody overnight at 4°C.

Western blot - Anti-LAS1L antibody (AB236954)
  • WB

Supplier Data

Western blot - Anti-LAS1L antibody (AB236954)

All lanes:

Western blot - Anti-LAS1L antibody (ab236954) at 1/500 dilution

All lanes:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human LAS1L aa 450-600. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4W2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAS1L also known as LAS1-like protein is a nucleolar protein with a molecular mass of approximately 80 kDa. It is expressed widely across human tissues with prominent presence in actively dividing cells. Mechanically LAS1L assists in the processing and maturation of pre-ribosomal RNA (rRNA) playing a critical role in ribosome biogenesis. This protein functions as part of a multi-protein complex ensuring precise assembly and function of ribosomes.
Biological function summary

LAS1L serves significant functions in the nucleolus within cell nucleuses. As a component of a larger complex called the pre-ribosome LAS1L coordinates with other proteins to facilitate the cleavage of pre-rRNA into smaller ribosomal RNA units. This activity is essential for producing functional ribosomal units which are important for protein synthesis. Its interaction with proteins such as GTPases and endonucleases is important for maintaining ribosome assembly fidelity.

Pathways

LAS1L operates within the ribosome biogenesis pathway closely interacting with proteins like PELP1 and NOL9. It plays a role in the mTOR signaling pathway impacting cell growth and proliferation. The protein also helps regulate downstream processes critical for the final assembly of ribosomal subunits integrating cellular nutrient signals with ribosome production.

LAS1L shows connections to various cancers and ribosomopathies. Alterations in LAS1L expression or function can lead to disrupted ribosomal biogenesis contributing to cancer pathogenesis due to uncontrolled cell division. Moreover LAS1L is linked with Diamond-Blackfan Anemia a disorder of ribosome production. Related proteins like NPM1 and RPL11 are involved in this condition highlighting the interplay between LAS1L and ribosome function-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA (PubMed : 20647540). Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed : 22872859). Required for the efficient pre-rRNA processing at both ends of internal transcribed spacer 2 (ITS2) (PubMed : 22083961).
See full target information LAS1L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 41:3347-3354 PubMed31002350

2019

Upregulated histamine receptor H3 promotes tumor growth and metastasis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Deliang Yu,Jing Zhao,Yuhong Wang,Jing Hu,Qinchuan Zhao,Jibin Li,Jianjun Zhu
View all publications
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Product promise

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