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AB140656

Anti-LAS1L antibody [EPR8988(B)]

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(2 Publications)

Rabbit Recombinant Monoclonal LAS1L antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

MSTP060, LAS1L, Ribosomal biogenesis protein LAS1L, Endoribonuclease LAS1L, Protein LAS1 homolog

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-LAS1L antibody [EPR8988(B)] (AB140656)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LAS1L antibody [EPR8988(B)] (AB140656)

Immunofluorescent analysis of HeLa cells labelling LAS1L with ab140656 at 1/250 dilution.

Western blot - Anti-LAS1L antibody [EPR8988(B)] (AB140656)
  • WB

Unknown

Western blot - Anti-LAS1L antibody [EPR8988(B)] (AB140656)

All lanes:

Western blot - Anti-LAS1L antibody [EPR8988(B)] (ab140656) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

PC-3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

  • Carrier free

    Anti-LAS1L antibody [EPR8988(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8988(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAS1L also known as LAS1-like protein is a nucleolar protein with a molecular mass of approximately 80 kDa. It is expressed widely across human tissues with prominent presence in actively dividing cells. Mechanically LAS1L assists in the processing and maturation of pre-ribosomal RNA (rRNA) playing a critical role in ribosome biogenesis. This protein functions as part of a multi-protein complex ensuring precise assembly and function of ribosomes.
Biological function summary

LAS1L serves significant functions in the nucleolus within cell nucleuses. As a component of a larger complex called the pre-ribosome LAS1L coordinates with other proteins to facilitate the cleavage of pre-rRNA into smaller ribosomal RNA units. This activity is essential for producing functional ribosomal units which are important for protein synthesis. Its interaction with proteins such as GTPases and endonucleases is important for maintaining ribosome assembly fidelity.

Pathways

LAS1L operates within the ribosome biogenesis pathway closely interacting with proteins like PELP1 and NOL9. It plays a role in the mTOR signaling pathway impacting cell growth and proliferation. The protein also helps regulate downstream processes critical for the final assembly of ribosomal subunits integrating cellular nutrient signals with ribosome production.

LAS1L shows connections to various cancers and ribosomopathies. Alterations in LAS1L expression or function can lead to disrupted ribosomal biogenesis contributing to cancer pathogenesis due to uncontrolled cell division. Moreover LAS1L is linked with Diamond-Blackfan Anemia a disorder of ribosome production. Related proteins like NPM1 and RPL11 are involved in this condition highlighting the interplay between LAS1L and ribosome function-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA (PubMed : 20647540). Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed : 22872859). Required for the efficient pre-rRNA processing at both ends of internal transcribed spacer 2 (ITS2) (PubMed : 22083961).
See full target information LAS1L
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular biology 431:3771-3786 PubMed31288032

2019

Nol9 Is a Spatial Regulator for the Human ITS2 Pre-rRNA Endonuclease-Kinase Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Jacob Gordon,Monica C Pillon,Robin E Stanley

Molecular & cellular proteomics : MCP 16:S200-S214 PubMed28077445

2017

Human Antiviral Protein IFIX Suppresses Viral Gene Expression during Herpes Simplex Virus 1 (HSV-1) Infection and Is Counteracted by Virus-induced Proteasomal Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Marni S Crow,Ileana M Cristea
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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