JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248934

Anti-LAS1L antibody [EPR8988(B)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal LAS1L antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MSTP060, LAS1L, Ribosomal biogenesis protein LAS1L, Endoribonuclease LAS1L, Protein LAS1 homolog

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-LAS1L antibody [EPR8988(B)] - BSA and Azide free (AB248934)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LAS1L antibody [EPR8988(B)] - BSA and Azide free (AB248934)

This data was developed using ab140656, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling LAS1L with ab140656 at 1/250 dilution.

Western blot - Anti-LAS1L antibody [EPR8988(B)] - BSA and Azide free (AB248934)
  • WB

Unknown

Western blot - Anti-LAS1L antibody [EPR8988(B)] - BSA and Azide free (AB248934)

This data was developed using ab140656, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LAS1L antibody [EPR8988(B)] (<a href='/en-us/products/primary-antibodies/las1l-antibody-epr8988b-ab140656'>ab140656</a>) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

PC-3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8988(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB248893

Anti-NOL9 antibody [EPR9578] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-NOL9 antibody [EPR9578] - BSA and Azide free (AB248893)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB69933

Anti-ZNF148 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF148 antibody (AB69933)

  • 4
  • 1
Primary Antibodies

AB181152

Anti-ZCCHC8 antibody [EPR13612]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC8 antibody [EPR13612] (AB181152)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab248934 is the carrier-free version of ab140656.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAS1L also known as LAS1-like protein is a nucleolar protein with a molecular mass of approximately 80 kDa. It is expressed widely across human tissues with prominent presence in actively dividing cells. Mechanically LAS1L assists in the processing and maturation of pre-ribosomal RNA (rRNA) playing a critical role in ribosome biogenesis. This protein functions as part of a multi-protein complex ensuring precise assembly and function of ribosomes.
Biological function summary

LAS1L serves significant functions in the nucleolus within cell nucleuses. As a component of a larger complex called the pre-ribosome LAS1L coordinates with other proteins to facilitate the cleavage of pre-rRNA into smaller ribosomal RNA units. This activity is essential for producing functional ribosomal units which are important for protein synthesis. Its interaction with proteins such as GTPases and endonucleases is important for maintaining ribosome assembly fidelity.

Pathways

LAS1L operates within the ribosome biogenesis pathway closely interacting with proteins like PELP1 and NOL9. It plays a role in the mTOR signaling pathway impacting cell growth and proliferation. The protein also helps regulate downstream processes critical for the final assembly of ribosomal subunits integrating cellular nutrient signals with ribosome production.

LAS1L shows connections to various cancers and ribosomopathies. Alterations in LAS1L expression or function can lead to disrupted ribosomal biogenesis contributing to cancer pathogenesis due to uncontrolled cell division. Moreover LAS1L is linked with Diamond-Blackfan Anemia a disorder of ribosome production. Related proteins like NPM1 and RPL11 are involved in this condition highlighting the interplay between LAS1L and ribosome function-related diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA (PubMed : 20647540). Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed : 22872859). Required for the efficient pre-rRNA processing at both ends of internal transcribed spacer 2 (ITS2) (PubMed : 22083961).
See full target information LAS1L
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com