Rabbit Polyclonal LASP1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human LASP1 aa 100-150.
View Alternative Names
MLN50, LASP1, LIM and SH3 domain protein 1, LASP-1, Metastatic lymph node gene 50 protein, MLN 50
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASP1 antibody (AB117806)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling LASP1 with ab117806 at 1 μg/mL concentration. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
- IP
Unknown
Immunoprecipitation - Anti-LASP1 antibody (AB117806)
ab117806 at 1 μg/ml staining LASP1 by WB following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab117806 at 6 μg/mg lysate.
Lane 2; IP using an antibody to a downstream epitope.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.
All lanes:
Immunoprecipitation - Anti-LASP1 antibody (ab117806)
Predicted band size: 30 kDa
false
- WB
Unknown
Western blot - Anti-LASP1 antibody (AB117806)
All lanes:
Western blot - Anti-LASP1 antibody (ab117806) at 0.1 µg/mL
Lane 1:
Whole cell lysate from HeLa cells at 50 µg
Lane 2:
Whole cell lysate from HeLa cells at 15 µg
Lane 3:
Whole cell lysate from 293T cells at 50 µg
Lane 4:
Whole cell lysate from Jurkat cells at 50 µg
Predicted band size: 30 kDa
true
Exposure time: 30s
- WB
CiteAb
Western blot - Anti-LASP1 antibody (AB117806)
LASP1 western blot using anti-LASP1 antibody ab117806. Publication image and figure legend from Hu, J. J., Liu, Y. W., et al., 2014, Proteome Sci, PubMed 24580839.
ab117806 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab117806 please see the product overview.
Confirmation of RNA and protein expression levels by real-time quantitative PCR and western blot analysis (n = 3, per treatment type). (A) The expression of LASP1 and ferritin light and heavy chain mRNAs relative to that of GAPDH, the internal control, as detected by real-time PCR. (B) Expression of LASP1 and ferritin light and heavy chains, as assessed by western blot analysis. The signal corresponding to the protein bands was quantified using densitometric scanning and the relative protein abundance was determined after normalization with the levels of β-actin protein. Data are presented as mean ± SD and compared using the t-test for independent samples. *p <0.05, indicating significant differences between the two samples compared.
false
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LASP1 plays a significant role in cell migration and adhesion processes. It associates with cytoskeletal proteins such as F-actin helping in the stabilization and organization of the cytoskeleton. LASP1 forms part of complexes with zyxin and vasodilator-stimulated phosphoprotein (VASP) at focal adhesions contributing to cellular motility and signaling. It influences processes like cytokinesis and wound healing which rely on precise cytoskeletal rearrangements.
Pathways
Researchers have identified LASP1 as being involved in the ERK/MAPK and PI3K/AKT signaling pathways. It links to these pathways through interactions promoting cell proliferation and survival. This protein associates with other pathway-related proteins like Grb2 and Src which play roles in transmitting signals from cell surface receptors to the nucleus impacting gene expression and cell cycle progression.
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Target data
Publications (5)
Recent publications for all applications. Explore the full list and refine your search
Cancers 15: PubMed37345118
2023
Applications
Unspecified application
Species
Unspecified reactive species
Open life sciences 16:523-536 PubMed34124372
2021
Applications
Unspecified application
Species
Unspecified reactive species
Gastroenterology research and practice 2020:8834359 PubMed33376485
2020
Applications
Unspecified application
Species
Unspecified reactive species
The Journal of biological chemistry 292:5801-5813 PubMed28232485
2017
Applications
Unspecified application
Species
Unspecified reactive species
Proteome science 12:13 PubMed24580839
2014
Applications
WB
Species
Dog
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