AB156872

Anti-LASP1 antibody [EPR11221]

RabMAb
Recombinant
What is this?

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(15 Publications)

Rabbit Recombinant Monoclonal LASP1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 15 publications.

View Alternative Names

MLN50, LASP1, LIM and SH3 domain protein 1, LASP-1, Metastatic lymph node gene 50 protein, MLN 50

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-LASP1 antibody [EPR11221] (AB156872)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LASP1 antibody [EPR11221] (AB156872)

Immunofluorescent analysis of A431 cells labeling LASP1 with ab156872 at 1/100.

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Western blot - Anti-LASP1 antibody [EPR11221] (AB156872)

All lanes:

Western blot - Anti-LASP1 antibody [EPR11221] (ab156872) at 1/10000 dilution

Lane 1:

SK-OV-3 cell lysates at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

U87-MG cell lysates at 10 µg

Lane 4:

293T cell lysates at 10 µg

Predicted band size: 30 kDa

false

Carrier free

Anti-LASP1 antibody [EPR11221] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11221

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LASP1 protein also known as LIM and SH3 protein 1 is a 261 amino acid protein with an approximate mass of 29 kDa. It acts as an actin-binding protein involved in the dynamics of the cytoskeleton. LASP1 is expressed in various tissues with higher expression seen in the kidneys lungs and breast tissues. Its structure includes an LIM domain at the N-terminal and an SH3 domain at the C-terminal essential for interactions with other proteins and cytoskeletal components.

Biological function summary

LASP1 plays a significant role in cell migration and adhesion processes. It associates with cytoskeletal proteins such as F-actin helping in the stabilization and organization of the cytoskeleton. LASP1 forms part of complexes with zyxin and vasodilator-stimulated phosphoprotein (VASP) at focal adhesions contributing to cellular motility and signaling. It influences processes like cytokinesis and wound healing which rely on precise cytoskeletal rearrangements.

Pathways

Researchers have identified LASP1 as being involved in the ERK/MAPK and PI3K/AKT signaling pathways. It links to these pathways through interactions promoting cell proliferation and survival. This protein associates with other pathway-related proteins like Grb2 and Src which play roles in transmitting signals from cell surface receptors to the nucleus impacting gene expression and cell cycle progression.

LASP1 overexpression often associates with various cancers particularly breast cancer and colorectal cancer. In these contexts LASP1 contributes to tumor progression and metastasis. Its interaction with proteins such as EGFR and HER2 has widespread implications in oncogenic signaling. Understanding LASP1's role in disease can enhance diagnostic and therapeutic strategies targeting these malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).

See full target information LASP1

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 32:1747-1763 PubMed39449799

2024

CircTIAM1 overexpression promotes the progression of papillary thyroid cancer by regulating the miR-338-3p/LASP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Y E Zhang,Yanan Liang,Yan Wu,Liwen Song,Zuwang Zhang

Oncology letters 28:306 PubMed38774456

2024

lncRNA PAARH impacts liver cancer cell proliferation by engaging miR‑6512‑3p to target LASP1.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wei,Guoman Liu,Zihua Huang,Jiahui Nian,Lizheng Huang,Yanyan Huang,Zheng Huang,Jian Pu

Oncology research 29:251-262 PubMed37303940

2023

Long noncoding RNA PPP1R14B-AS1 imitates microRNA-134-3p to facilitate breast cancer progression by upregulating LIM and SH3 protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Limin Zhou,Lianbo Zhang,Xin Guan,Y I Dong,Tao Liu

Neoplasma 69:1406-1417 PubMed36591804

2023

LncRNA CBR3-AS1 predicts a poor prognosis and promotes cervical cancer progression through the miR-3163/LASP1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yipin Cai,Yu Huang,Jie Zhang,Xiaoli Liu,Fang Zhao,Kuan Zhang,Songyuan Xu,Yan Yang,Haiwei Huang,Yu Gao

Histology and histopathology 38:585-595 PubMed36394255

2022

Circular RNA hsa_circ_0003892 promotes the development of papillary thyroid carcinoma by regulating the miR-326/LASP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Han,Junsong Liu,Qian Zhao,Honghui Li,Ting Zhang,Baiya Li,Xiaorong Niu

Journal of clinical laboratory analysis 36:e24304 PubMed35312115

2022

Hsa_circ_0007142 contributes to cisplatin resistance in esophageal squamous cell carcinoma via miR-494-3p/LASP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Na Chang,Ning Ge,Yufei Zhao,Liu Yang,Wei Qin,Yayun Cui

Frontiers in oncology 11:683915 PubMed34976784

2021

Development and Validation of an Immune-Related Signature for the Prediction of Recurrence Risk of Patients With Laryngeal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zhang,Xudong Zhao,Jin Wang,Wenyue Ji

Oncology reports 46: PubMed34328197

2021

Long non‑coding RNA H19 regulates LASP1 expression in osteosarcoma by competitively binding to miR‑29a‑3p.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Jin,Huan Wang,Xin Jin,Wenbo Wang

Digestive diseases and sciences 67:2158-2172 PubMed34024023

2021

Circular RNA circ_0006948 Promotes Esophageal Squamous Cell Carcinoma Progression by Regulating microRNA-3612/LASP1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rongzhu Tang,Qiang Zhou,Qihua Xu,Lihua Lu,Ying Zhou

IUBMB life 72:1960-1975 PubMed32726517

2020

Long non-coding RNA LINC01207 promotes prostate cancer progression by downregulating microRNA-1972 and upregulating LIM and SH3 protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Sugui Wang,Jianguo Qiu,Liping Wang,Ziyu Wu,Xianyun Zhang,Qiang Li,Fujin Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LASP1 antibody [EPR11221]

Immunocytochemistry/ Immunofluorescence - Anti-LASP1 antibody [EPR11221] (AB156872)

Western blot - Anti-LASP1 antibody [EPR11221] (AB156872)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise