AB191022

Anti-LASP1 antibody - N-terminal

Name: Anti-LASP1 antibody - N-terminal (ab191022) | Abcam
Brand: Abcam Limited
SKU: AB191022
Price: 535 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal LASP1 antibody. N-terminal. Suitable for IHC-P, ICC, WB and reacts with Human, Rat samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human LASP1 aa 1-50.

View Alternative Names

MLN50, LASP1, LIM and SH3 domain protein 1, LASP-1, Metastatic lymph node gene 50 protein, MLN 50

3 Images

Supplier Data

Immunocytochemistry - Anti-LASP1 antibody - N-terminal (AB191022)

Immunocytochemical analysis of A549 cells, staining LASP1 with ab191022 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASP1 antibody - N-terminal (AB191022)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASP1 antibody - N-terminal (AB191022)

Immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded human lung cancer tissue labelling LASP1 with ab191022 at 1 μg/ml.

Supplier Data

Western blot - Anti-LASP1 antibody - N-terminal (AB191022)

All lanes:

Western blot - Anti-LASP1 antibody - N-terminal (ab191022) at 0.5 µg/mL

Lane 1:

Rat liver tissue lysate

Lane 2:

Rat spleen tissue lysate

Lane 3:

Rat intestine tissue lysate

Lane 4:

Jurkat cell lysate

Lane 5:

MCF7 cell lysate

Lane 6:

A431 cell lysate

Lane 7:

HeLa cell lysate

Lane 8:

293T cell lysate

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, ICC, WB

applications

Immunogen

Synthetic Peptide within Human LASP1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q14847

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.5-1 µg/mL", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LASP1 protein also known as LIM and SH3 protein 1 is a 261 amino acid protein with an approximate mass of 29 kDa. It acts as an actin-binding protein involved in the dynamics of the cytoskeleton. LASP1 is expressed in various tissues with higher expression seen in the kidneys lungs and breast tissues. Its structure includes an LIM domain at the N-terminal and an SH3 domain at the C-terminal essential for interactions with other proteins and cytoskeletal components.

Biological function summary

LASP1 plays a significant role in cell migration and adhesion processes. It associates with cytoskeletal proteins such as F-actin helping in the stabilization and organization of the cytoskeleton. LASP1 forms part of complexes with zyxin and vasodilator-stimulated phosphoprotein (VASP) at focal adhesions contributing to cellular motility and signaling. It influences processes like cytokinesis and wound healing which rely on precise cytoskeletal rearrangements.

Pathways

Researchers have identified LASP1 as being involved in the ERK/MAPK and PI3K/AKT signaling pathways. It links to these pathways through interactions promoting cell proliferation and survival. This protein associates with other pathway-related proteins like Grb2 and Src which play roles in transmitting signals from cell surface receptors to the nucleus impacting gene expression and cell cycle progression.

LASP1 overexpression often associates with various cancers particularly breast cancer and colorectal cancer. In these contexts LASP1 contributes to tumor progression and metastasis. Its interaction with proteins such as EGFR and HER2 has widespread implications in oncogenic signaling. Understanding LASP1's role in disease can enhance diagnostic and therapeutic strategies targeting these malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).

See full target information LASP1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Balkan medical journal 39:411-421 PubMed36172827

2022

Circ_0001535 Facilitates Tumor Malignant Progression by miR-485-5p/LASP1 Axis in Colorectal Cancer

Applications

Unspecified application

Species

Unspecified reactive species

Liang Bai,Zhifeng Gao,An Jiang,Song Ren,Baotai Wang

Reproductive biology and endocrinology : RB&E 20:64 PubMed35379225

2022

miR-218-5p in endometrial microenvironment prevents the migration of ectopic endometrial stromal cells by inhibiting LASP1.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyu Zhang,Yaoqing Wang,Liqin Zeng,Kaihui Yu,Yuanqin Wang,Yong Luo,Faying Liu,Bicheng Yang,Yang Zou,Liqun Wang,Ouping Huang

Molecular human reproduction : PubMed33543750

2021

Endometrial stromal cell proteomic analysis reveals LIM and SH3 protein 1 (LASP1) plays important roles in the progression of adenomyosis.

Applications

Unspecified application

Species

Unspecified reactive species

Faying Liu,Zengming Li,Jiubai Guo,Shufen Fang,Jiangyan Zhou,Bianna Cao,Jun Liu,Yulan Yi,Xiaoqun Yuan,Xiaoyun Xu,Ouping Huang,Liqun Wang,Yang Zou

American journal of translational research 12:6490-6500 PubMed33194046

2020

LASP1 induces colorectal cancer proliferation and invasiveness through Hippo signaling and Nanog mediated EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Na Chen,Xiangdong Han,Xue Bai,Bo Yin,Yubin Wang

Cell proliferation 52:e12564 PubMed30938030

2019

Suppression of long non-coding RNA TNRC6C-AS1 protects against thyroid carcinoma through DNA demethylation of STK4 via the Hippo signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liu-Xue Yang,Ji Wu,Man-Li Guo,Yong Zhang,Shao-Gang Ma

Oncotarget 8:75102-75113 PubMed29088849

2017

Lasp1 promotes malignant phenotype of non-small-cell lung cancer via inducing phosphorylation of FAK-AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiupeng Zhang,Yang Liu,Chuifeng Fan,Liang Wang,Ailin Li,Haijing Zhou,Lin Cai,Yuan Miao,Qingchang Li,Xueshan Qiu,Enhua Wang

Molecular cancer 15:72 PubMed27852308

2016

Systems analysis identifies miR-29b regulation of invasiveness in melanoma.

Applications

Species

Human

Miles C Andrews,Joseph Cursons,Daniel G Hurley,Matthew Anaka,Jonathan S Cebon,Andreas Behren,Edmund J Crampin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LASP1 antibody - N-terminal

Immunocytochemistry - Anti-LASP1 antibody - N-terminal (AB191022)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASP1 antibody - N-terminal (AB191022)

Western blot - Anti-LASP1 antibody - N-terminal (AB191022)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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