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Rabbit Monoclonal CERS2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CERS2.

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Images

Western blot - Anti-LASS2 antibody [HL1717] (AB308189), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (AB308189), expandable thumbnail
  • Western blot - Anti-LASS2 antibody [HL1717] (AB308189), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human CERS2. The exact immunogen used to generate this antibody is proprietary information. Database link Q96G23

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target data

Function

Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward very-long-chain fatty acyl-CoA (chain length C22-C27) (PubMed:17977534, PubMed:18165233, PubMed:18541923, PubMed:19728861, PubMed:20937905, PubMed:22144673, PubMed:22661289, PubMed:26887952, PubMed:29632068). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively (By similarity) (PubMed:17977534, PubMed:18165233, PubMed:18541923, PubMed:19728861, PubMed:20937905, PubMed:22144673, PubMed:22661289, PubMed:26887952, PubMed:29632068). Plays a non-redundant role in the synthesis of ceramides with very-long-chain fatty acids in kidney, liver and brain. Regulates the abundance of myelin-specific sphingolipids galactosylceramide and sulfatide that affects myelin sheath architecture and motor neuron functions (By similarity).

Alternative names

LASS2, TMSG1, CERS2, Ceramide synthase 2, CerS2, LAG1 longevity assurance homolog 2, SP260, Sphingosine N-acyltransferase CERS2, Tumor metastasis-suppressor gene 1 protein, Very-long-chain ceramide synthase CERS2

Recommended products

Rabbit Monoclonal CERS2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CERS2.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human CERS2. The exact immunogen used to generate this antibody is proprietary information. Database link Q96G23
Carrier free
Yes
Clone number
HL1717
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-LASS2 antibody [HL1717] (ab308189), expandable thumbnail

    Western blot - Anti-LASS2 antibody [HL1717] (ab308189)

    The signal was developed with Trident ECL plus-Enhanced

    All lanes: Western blot - Anti-LASS2 antibody [HL1717] (ab308189) at 1/1000 dilution

    Lane 1: Wild-type (WT) 293T cell extract at 30 µg

    Lane 2: LASS2 knockout (KO) 293T cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 45 kDa

  • Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (ab308189), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (ab308189)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling LASS2 protein with ab308189 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

  • Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (ab308189), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (ab308189)

    Immunohistochemical analysis of paraffin-embedded human lung cancer labeling LASS2 protein with ab308189 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (ab308189), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (ab308189)

    For immunofluorescence analysis, HeLa cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of LASS2 protein using ab308189 at 1/500 dilution (green).

    Nuclei (blue) is stained with fluoroshield with DAPI

  • Western blot - Anti-LASS2 antibody [HL1717] (ab308189), expandable thumbnail

    Western blot - Anti-LASS2 antibody [HL1717] (ab308189)

    Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody [HL1717] (ab308189) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

    All lanes: Western blot - Anti-LASS2 antibody [HL1717] (ab308189) at 1/1000 dilution

    Lane 1: HEK293T cell lysate at 30 µg

    Lane 2: A431 cell lysate at 30 µg

    Lane 3: HeLa cell lysate at 30 µg

    Lane 4: HepG2 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Developed using the ECL technique.

    Predicted band size: 44.9 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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