AB308189

Anti-LASS2 antibody [HL1717]

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal CERS2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CERS2.

View Alternative Names

LASS2, TMSG1, CERS2, Ceramide synthase 2, CerS2, LAG1 longevity assurance homolog 2, SP260, Sphingosine N-acyltransferase CERS2, Tumor metastasis-suppressor gene 1 protein, Very-long-chain ceramide synthase CERS2

5 Images

Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189)

Immunohistochemical analysis of paraffin-embedded human lung cancer labeling LASS2 protein with ab308189 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (AB308189)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (AB308189)

For immunofluorescence analysis, HeLa cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of LASS2 protein using ab308189 at 1/500 dilution (green).

Nuclei (blue) is stained with fluoroshield with DAPI

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling LASS2 protein with ab308189 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Supplier Data

Western blot - Anti-LASS2 antibody [HL1717] (AB308189)

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody [HL1717] (ab308189) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-LASS2 antibody [HL1717] (ab308189) at 1/1000 dilution

Lane 1:

HEK293T cell lysate at 30 µg

Lane 2:

A431 cell lysate at 30 µg

Lane 3:

HeLa cell lysate at 30 µg

Lane 4:

HepG2 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 44.9 kDa

true

Supplier Data

Western blot - Anti-LASS2 antibody [HL1717] (AB308189)

The signal was developed with Trident ECL plus-Enhanced

All lanes:

Western blot - Anti-LASS2 antibody [HL1717] (ab308189) at 1/1000 dilution

Lane 1:

Wild-type (WT) 293T cell extract at 30 µg

Lane 2:

LASS2 knockout (KO) 293T cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1717

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CERS2. The exact immunogen used to generate this antibody is proprietary information.

Q96G23

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward very-long-chain fatty acyl-CoA (chain length C22-C27) (PubMed : 17977534, PubMed : 18165233, PubMed : 18541923, PubMed : 19728861, PubMed : 20937905, PubMed : 22144673, PubMed : 22661289, PubMed : 26887952, PubMed : 29632068). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively (By similarity) (PubMed : 17977534, PubMed : 18165233, PubMed : 18541923, PubMed : 19728861, PubMed : 20937905, PubMed : 22144673, PubMed : 22661289, PubMed : 26887952, PubMed : 29632068). Plays a non-redundant role in the synthesis of ceramides with very-long-chain fatty acids in kidney, liver and brain. Regulates the abundance of myelin-specific sphingolipids galactosylceramide and sulfatide that affects myelin sheath architecture and motor neuron functions (By similarity).

See full target information CERS2

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LASS2 antibody [HL1717]

Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189)

Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (AB308189)

Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189)

Western blot - Anti-LASS2 antibody [HL1717] (AB308189)

Western blot - Anti-LASS2 antibody [HL1717] (AB308189)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Product promise