Rabbit Monoclonal CERS2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CERS2.
Images
![Western blot - Anti-LASS2 antibody [HL1717] (AB308189), expandable thumbnail](https://content.abcam.com/products/images/lass2-antibody-hl1717-ab308189--western-blot-img429487.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LASS2 antibody [HL1717] (AB308189)")
![Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189), expandable thumbnail](https://content.abcam.com/products/images/lass2-antibody-hl1717-ab308189--immunohistochemistry-paraffin-embedded-sections-img407278.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189)")
![Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189), expandable thumbnail](https://content.abcam.com/products/images/lass2-antibody-hl1717-ab308189--immunohistochemistry-paraffin-embedded-sections-img407277.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (AB308189)")
![Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (AB308189), expandable thumbnail](https://content.abcam.com/products/images/lass2-antibody-hl1717-ab308189--immunocytochemistry-immunofluorescence-img407275.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (AB308189)")
![Western blot - Anti-LASS2 antibody [HL1717] (AB308189), expandable thumbnail](https://content.abcam.com/products/images/lass2-antibody-hl1717-ab308189--western-blot-img407276.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LASS2 antibody [HL1717] (AB308189)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CERS2. The exact immunogen used to generate this antibody is proprietary information. Database link Q96G23
Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
Ceramide synthase that catalyzes formation of ceramide from sphinganine and acyl-CoA substrates, with high selectivity toward very-long (C22:0-C24:0) chain as acyl donor. May regulate lipid metabolism in hepatocytes (By similarity).
Alternative names
Ceramide synthase 2, CerS2, LAG1 longevity assurance homolog 2, SP260, Sphingosine N-acyltransferase CERS2, Tumor metastasis-suppressor gene 1 protein, LASS2, TMSG1, CERS2
Recommended products
Rabbit Monoclonal CERS2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CERS2.
Alternative names
Ceramide synthase 2, CerS2, LAG1 longevity assurance homolog 2, SP260, Sphingosine N-acyltransferase CERS2, Tumor metastasis-suppressor gene 1 protein, LASS2, TMSG1, CERS2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CERS2. The exact immunogen used to generate this antibody is proprietary information. Database link Q96G23
- Carrier free
Yes
- Clone number
HL1717
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-LASS2 antibody [HL1717] (ab308189), expandable thumbnail](https://content.abcam.com/products/images/lass2-antibody-hl1717-ab308189--western-blot-img429487.jpg "Western blot - Anti-LASS2 antibody [HL1717] (ab308189)")
Western blot - Anti-LASS2 antibody [HL1717] (ab308189)
The signal was developed with Trident ECL plus-Enhanced
All lanes: Western blot - Anti-LASS2 antibody [HL1717] (ab308189) at 1/1000 dilution
Lane 1: Wild-type (WT) 293T cell extract at 30 µg
Lane 2: LASS2 knockout (KO) 293T cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 45 kDa
![Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (ab308189), expandable thumbnail](https://content.abcam.com/products/images/lass2-antibody-hl1717-ab308189--immunohistochemistry-paraffin-embedded-sections-img407278.jpg "Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (ab308189)")
Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (ab308189)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling LASS2 protein with ab308189 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.
![Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (ab308189), expandable thumbnail](https://content.abcam.com/products/images/lass2-antibody-hl1717-ab308189--immunohistochemistry-paraffin-embedded-sections-img407277.jpg "Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (ab308189)")
Immunohistochemistry paraffin embedded sections - Anti-LASS2 antibody [HL1717] (ab308189)
Immunohistochemical analysis of paraffin-embedded human lung cancer labeling LASS2 protein with ab308189 at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (ab308189), expandable thumbnail](https://content.abcam.com/products/images/lass2-antibody-hl1717-ab308189--immunocytochemistry-immunofluorescence-img407275.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (ab308189)")
Immunocytochemistry/ Immunofluorescence - Anti-LASS2 antibody [HL1717] (ab308189)
For immunofluorescence analysis, HeLa cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of LASS2 protein using ab308189 at 1/500 dilution (green).
Nuclei (blue) is stained with fluoroshield with DAPI
![Western blot - Anti-LASS2 antibody [HL1717] (ab308189), expandable thumbnail](https://content.abcam.com/products/images/lass2-antibody-hl1717-ab308189--western-blot-img407276.jpg "Western blot - Anti-LASS2 antibody [HL1717] (ab308189)")
Western blot - Anti-LASS2 antibody [HL1717] (ab308189)
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody [HL1717] (ab308189) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
All lanes: Western blot - Anti-LASS2 antibody [HL1717] (ab308189) at 1/1000 dilution
Lane 1: HEK293T cell lysate at 30 µg
Lane 2: A431 cell lysate at 30 µg
Lane 3: HeLa cell lysate at 30 µg
Lane 4: HepG2 cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 44.9 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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