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AB169259

Anti-LASS3 antibody

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(2 Publications)

Mouse Polyclonal LASS3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CERS3.

View Alternative Names

LASS3, CERS3, Ceramide synthase 3, CerS3, Dihydroceramide synthase 3, LAG1 longevity assurance homolog 3, Sphingosine N-acyltransferase CERS3, Ultra-long-chain ceramide synthase CERS3, Very-long-chain ceramide synthase CERS3

1 Images
Western blot - Anti-LASS3 antibody (AB169259)
  • WB

Unknown

Western blot - Anti-LASS3 antibody (AB169259)

All lanes:

Western blot - Anti-LASS3 antibody (ab169259) at 1 µg/mL

Lane 1:

LASS3 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 46 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CERS3.

Q8IU89

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LASS3 also known as Ceramide Synthase 3 or CERS3 is a protein that catalyzes the synthesis of ceramides essential components of lipid metabolism. It exhibits a molecular mass of approximately 45 kDa. LASS3 is expressed in various tissues but shows a higher expression in the skin indicating a potential role in dermatological functions. Furthermore LASS3 resides mainly in the endoplasmic reticulum where it executes its enzymatic functions efficiently.
Biological function summary

Ceramide synthesis by LASS3 contributes significantly to the maintenance of skin barrier functions and epidermal keratinization processes. LASS3 acts as part of the larger ceramide synthase family which forms ceramide as a central molecule in sphingolipid metabolism. Keratinocytes display a reliance on LASS3 activity to produce long-chain ceramides which are key in forming the lipid matrix of the skin.

Pathways

LASS3 participates in the sphingolipid metabolic pathway working alongside other ceramide synthases like LASS1 and LASS2. It contributes to the formation of ceramides involved in cell signaling and apoptosis pathways. LASS3's activity links to the Protein Kinase C signaling pathway which regulates cell proliferation and differentiation by interacting with PKC isoforms influencing downstream signaling events.

LASS3 plays a role in skin-related conditions such as ichthyosis and psoriasis. Its involvement in ceramide synthesis connects it to these disorders through its impact on epidermal barrier restoration and inflammation processes. Additionally disruptions in LASS3 function have a connection to altered expressions of proteins like filaggrin which is significant in ichthyosis vulgaris signaling how vital LASS3 is in skin health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward very- and ultra-long-chain fatty acyl-CoA (chain length greater than C22) (PubMed : 17977534, PubMed : 22038835, PubMed : 26887952). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively (PubMed : 17977534, PubMed : 22038835, PubMed : 26887952). It is crucial for the synthesis of ultra-long-chain ceramides in the epidermis, to maintain epidermal lipid homeostasis and terminal differentiation (PubMed : 23754960).
See full target information CERS3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed36831084

2023

Horse-Derived Ceramide Accentuates Glucosylceramide Synthase and Ceramide Synthase 3 by Activating PPARβ/δ and/or PPARγ to Stimulate Ceramide Synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tami Igarashi,Hiroki Yanagi,Masayuki Yagi,Masamitsu Ichihashi,Genji Imokawa

The American journal of surgical pathology 40:224-35 PubMed26501226

2015

A Molecular Study of Pediatric Spindle and Sclerosing Rhabdomyosarcoma: Identification of Novel and Recurrent VGLL2-related Fusions in Infantile Cases.

Applications

Unspecified application

Species

Unspecified reactive species

Rita Alaggio,Lei Zhang,Yun-Shao Sung,Shih-Chiang Huang,Chun-Liang Chen,Gianni Bisogno,Angelica Zin,Narasimhan P Agaram,Michael P LaQuaglia,Leonard H Wexler,Cristina R Antonescu
View all publications
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Product promise

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