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AB272552

Anti-LASS3 antibody

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Rabbit Polyclonal LASS3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CERS3 aa 50-150.

View Alternative Names

LASS3, CERS3, Ceramide synthase 3, CerS3, Dihydroceramide synthase 3, LAG1 longevity assurance homolog 3, Sphingosine N-acyltransferase CERS3, Ultra-long-chain ceramide synthase CERS3, Very-long-chain ceramide synthase CERS3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS3 antibody (AB272552)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS3 antibody (AB272552)

Paraffin-embedded formalin-fixed human skeletal tissue stained for LASS3 using ab272552 at 1/50 dilution in immunohistochemical analysis. Shows low expression as expected.

Immunocytochemistry/ Immunofluorescence - Anti-LASS3 antibody (AB272552)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LASS3 antibody (AB272552)

PFA fixed, Triton X-100 permeablized U-251 MG cells (human brain glioma cell line) labeling LASS3 (green) using ab272552at 2 ug/ml in ICC/IF analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS3 antibody (AB272552)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS3 antibody (AB272552)

Paraffin-embedded formalin-fixed human skin tissue stained for LASS3 using ab272552 at 1/50 dilurion in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS3 antibody (AB272552)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LASS3 antibody (AB272552)

Paraffin-embedded formalin-fixed human testis tissue stained for LASS3 using ab272552 at 1/50 dilurion in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CERS3 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q8IU89

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LASS3 also known as Ceramide Synthase 3 or CERS3 is a protein that catalyzes the synthesis of ceramides essential components of lipid metabolism. It exhibits a molecular mass of approximately 45 kDa. LASS3 is expressed in various tissues but shows a higher expression in the skin indicating a potential role in dermatological functions. Furthermore LASS3 resides mainly in the endoplasmic reticulum where it executes its enzymatic functions efficiently.
Biological function summary

Ceramide synthesis by LASS3 contributes significantly to the maintenance of skin barrier functions and epidermal keratinization processes. LASS3 acts as part of the larger ceramide synthase family which forms ceramide as a central molecule in sphingolipid metabolism. Keratinocytes display a reliance on LASS3 activity to produce long-chain ceramides which are key in forming the lipid matrix of the skin.

Pathways

LASS3 participates in the sphingolipid metabolic pathway working alongside other ceramide synthases like LASS1 and LASS2. It contributes to the formation of ceramides involved in cell signaling and apoptosis pathways. LASS3's activity links to the Protein Kinase C signaling pathway which regulates cell proliferation and differentiation by interacting with PKC isoforms influencing downstream signaling events.

LASS3 plays a role in skin-related conditions such as ichthyosis and psoriasis. Its involvement in ceramide synthesis connects it to these disorders through its impact on epidermal barrier restoration and inflammation processes. Additionally disruptions in LASS3 function have a connection to altered expressions of proteins like filaggrin which is significant in ichthyosis vulgaris signaling how vital LASS3 is in skin health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward very- and ultra-long-chain fatty acyl-CoA (chain length greater than C22) (PubMed : 17977534, PubMed : 22038835, PubMed : 26887952). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively (PubMed : 17977534, PubMed : 22038835, PubMed : 26887952). It is crucial for the synthesis of ultra-long-chain ceramides in the epidermis, to maintain epidermal lipid homeostasis and terminal differentiation (PubMed : 23754960).
See full target information CERS3
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Product promise

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