AB73289

Anti-Lass5 antibody

Name: Anti-Lass5 antibody (ab73289) | Abcam
Brand: Abcam Limited
SKU: AB73289

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(4 Publications )

Rabbit Polyclonal Lass5 antibody. Suitable for WB and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CERS5.

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Western blot - Anti-Lass5 antibody (AB73289)

Lane 1:

Western blot - Anti-Lass5 antibody (ab73289) at 1 µg/mL

Lane 2:

Western blot - Anti-Lass5 antibody (ab73289) at 2 µg/mL

All lanes:

Rat brain tissue lysate at 15 µg

Predicted band size: 46 kDa

Observed band size: 46 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

applications

Immunogen

Synthetic Peptide within Human CERS5. The exact immunogen used to generate this antibody is proprietary information.

Q8N5B7

Specificity

ab73289 may cross-react with the highly homologous Lass6.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lass5 also known as ceramide synthase 5 (CerS5) plays a critical role in ceramide production by catalyzing the condensation of sphinganine or sphingosine with palmitoyl-CoA. It has a molecular mass of approximately 42 kDa. Lass5 is primarily expressed in the liver heart and skeletal muscle and it is found in the endoplasmic reticulum membrane. This enzyme displays a preference for producing C16-ceramide due to its palmitoyl-CoA specificity.

Biological function summary

Lass5 contributes significantly to the regulation of cell proliferation apoptosis and response to stress. Lass5 forms part of a larger complex involved in sphingolipid metabolism which includes other ceramide synthases such as CerS2 and CerS4. By controlling ceramide levels Lass5 can influence cellular and physiological functions like autophagy and cell cycle arrest.

Pathways

Lass5 is heavily involved in the sphingolipid signaling pathway and the endoplasmic reticulum stress response pathway. These pathways are important for cell survival and homeostasis. Lass5 interacts with key proteins such as Bcl-2 family members which are critical for apoptosis regulation. It works together with other ceramide synthases to maintain cellular homeostasis and mediate various biological processes impacting cell function and survival.

Lass5 has been linked to cancer and neurodegenerative diseases. Overexpression or dysregulation of Lass5 can result in abnormal ceramide accumulation contributing to diseases like hepatocellular carcinoma. It may also play a role in the progression of neurodegenerative disorders such as Alzheimer's disease possibly through interactions with proteins like amyloid precursor protein (APP) in the neuronal context. Understanding Lass5's influence in these conditions can help in developing therapeutic strategies that target its function or regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward palmitoyl-CoA (hexadecanoyl-CoA; C16 : 0-CoA) (PubMed : 16951403, PubMed : 18541923, PubMed : 22144673, PubMed : 22661289, PubMed : 23530041, PubMed : 26887952, PubMed : 29632068, PubMed : 31916624). Can use other acyl donors, but with less efficiency (By similarity). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively (PubMed : 31916624). Plays a role in de novo ceramide synthesis and surfactant homeostasis in pulmonary epithelia (By similarity).

See full target information CERS5

Alternative Names

LASS5, CERS5, Ceramide synthase 5, CerS5, LAG1 longevity assurance homolog 5, Sphingoid base N-palmitoyltransferase CERS5, Sphingosine N-acyltransferase CERS5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadf2746 PubMed37390205

2023

A localized hydrogel-mediated chemotherapy causes immunogenic cell death via activation of ceramide-mediated unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Animesh Kar,Dolly Jain,Sandeep Kumar,Kajal Rajput,Sanjay Pal,Kajal Rana,Raunak Kar,Somesh K Jha,Nihal Medatwal,Prabhu Srinivas Yavvari,Nishant Pandey,Devashish Mehta,Harsh Sharma,Debanjan Bhattacharya,Manas K Pradhan,Ravi Datta Sharma,Aasheesh Srivastava,Usha Agrawal,Arnab Mukhopadhyay,Sagar Sengupta,Veena S Patil,Avinash Bajaj,Ujjaini Dasgupta

Cell death & disease 12:171 PubMed33568634

2021

Alternative splicing of ceramide synthase 2 alters levels of specific ceramides and modulates cancer cell proliferation and migration in Luminal B breast cancer subtype.

Applications

Unspecified application

Species

Unspecified reactive species

Trishna Pani,Kajal Rajput,Animesh Kar,Harsh Sharma,Rituparna Basak,Nihal Medatwal,Sandhini Saha,Gagan Dev,Sharwan Kumar,Siddhi Gupta,Arnab Mukhopadhyay,Dipankar Malakar,Tushar Kanti Maiti,Aneeshkumar G Arimbasseri,S V S Deo,Ravi Datta Sharma,Avinash Bajaj,Ujjaini Dasgupta

Biomolecules 10: PubMed32325909

2020

Ceramides Profile Identifies Patients with More Advanced Stages of Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Adam R Markowski,Agnieszka U Błachnio-Zabielska,Katarzyna Guzińska-Ustymowicz,Agnieszka Markowska,Karolina Pogodzińska,Kamila Roszczyc,Justyna Zińczuk,Piotr Zabielski

JCI insight 2: PubMed28469091

2017

Increased de novo ceramide synthesis and accumulation in failing myocardium.

Applications

Species

Human

Ruiping Ji,Hirokazu Akashi,Konstantinos Drosatos,Xianghai Liao,Hongfeng Jiang,Peter J Kennel,Danielle L Brunjes,Estibaliz Castillero,Xiaokan Zhang,Lily Y Deng,Shunichi Homma,Isaac J George,Hiroo Takayama,Yoshifumi Naka,Ira J Goldberg,P Christian Schulze

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Lass5 antibody

Western blot - Anti-Lass5 antibody (AB73289)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (4)

Product promise