JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308422

Anti-Lassa virus Nucleoprotein antibody [HL1390]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Lassa virus Nucleoprotein antibody. Carrier free. Suitable for WB and reacts with Transfected cell line - Mammarenavirus lassaense samples.
1 Images
Western blot - Anti-Lassa virus Nucleoprotein antibody [HL1390] (AB308422)
  • WB

Supplier Data

Western blot - Anti-Lassa virus Nucleoprotein antibody [HL1390] (AB308422)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lassa virus Nucleoprotein antibody [HL1390] (ab308422) diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Lassa virus Nucleoprotein antibody [HL1390] (ab308422) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1390

Isotype

IgG

Carrier free

Yes

Reacts with

Mammarenavirus lassaense

Applications

WB

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mammarenavirus lassaense": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Mammarenavirus lassaense": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LASV NP also known as Lassa virus nucleoprotein or NP is a structural protein with a mass of approximately 63 kDa. It plays an important role in the replication cycle of Lassa virus a member of the Arenaviridae family. Expressed primarily in infected host cells NP assists in the formation of viral ribonucleoprotein complexes. This protein encapsidates the viral RNA genome safeguarding it from degradation and interacting with the viral polymerase which is necessary for efficient viral replication and transcription.
Biological function summary

This nucleoprotein serves a multifunctional role in viral propagation and immune evasion. In addition to its RNA-binding properties NP suppresses the host's innate immune responses by interfering with interferon signaling pathways. It accomplishes this by degrading double-stranded RNA thereby preventing activation of the pathway. NP is not known to be part of a larger complex but operates closely with other viral components to enhance viral survival and replication within host cells.

Pathways

LASV NP is implicated importantly in the viral life cycle and immune evasion mechanisms. The interferon signaling pathway is significantly impacted by NP's ability to counteract interferon responses which normally aid in defense against viral infections. NP cooperates with the Lassa virus glycoprotein complex and matrix protein in these pathways coordinating processes that facilitate successful viral replication and assembly while concurrently subverting host immune defenses.

LASV NP is closely associated with Lassa fever a severe viral hemorrhagic illness endemic in parts of West Africa. The NP's role in immune system modulation connects it with an increased virulence of the virus therefore contributing to the pathogenesis of Lassa fever. Additionally interactions between NP and the host immune-modulating proteins such as those in the interferon family influence the disease progression and host immune response making it a target of interest for antiviral therapies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Lassa virus Nucleoprotein
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com