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AB308363

Anti-Lassa virus Nucleoprotein antibody [HL1392]

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Rabbit Recombinant Monoclonal NCAP antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - Mammarenavirus lassaense samples. Immunogen corresponding to Synthetic Peptide within Lassa virus Josiah N.

View Alternative Names

Nucleoprotein, Nucleocapsid protein, Protein N, N

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Lassa virus Nucleoprotein antibody [HL1392] (AB308363)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Lassa virus Nucleoprotein antibody [HL1392] (AB308363)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Lassa virus Nucleoprotein using ab308363 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Western blot - Anti-Lassa virus Nucleoprotein antibody [HL1392] (AB308363)
  • WB

Supplier Data

Western blot - Anti-Lassa virus Nucleoprotein antibody [HL1392] (AB308363)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lassa virus Nucleoprotein antibody [HL1392] (ab308363) diluted at 1/300000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1392

Isotype

IgG

Carrier free

Yes

Reacts with

Mammarenavirus lassaense

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Lassa virus Josiah N. The exact immunogen used to generate this antibody is proprietary information.

P13699

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mammarenavirus lassaense": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Mammarenavirus lassaense": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/100000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nucleoprotein also known as NP is a structural protein that plays an important role in the assembly and genomic encapsidation of several viruses including influenza and hantavirus. It has a molecular mass that varies depending on the specific virus but typically ranges from 50 to 60 kDa. Nucleoprotein is expressed predominantly within the nuclear and cytoplasmic regions of infected host cells. It interacts directly with the viral RNA forming a ribonucleoprotein complex that facilitates the viral replication process.
Biological function summary

Nucleoprotein acts as an essential component in the viral lifecycle by aiding in the protection and packaging of viral RNA. It typically associates with other viral proteins to form a ribonucleoprotein complex which is necessary for viral replication and transcription. This complex ensures the encapsidation of the viral genome protecting it from nucleases and allowing proper assembly into new virions. Additionally nucleoprotein has been found to modulate host immune responses potentially aiding viral evasion of host defense mechanisms.

Pathways

Nucleoprotein participates in viral replication and transcription pathways particularly within the context of the host-virus interaction. It is associated with the replication machinery of the virus where it interacts with the polymerase complex which includes proteins such as polymerase basic 1 (PB1) and polymerase basic 2 (PB2). This interaction facilitates the synthesis of complementary RNA strands necessary for viral propagation and the production of viral proteins.

Nucleoprotein’s involvement is significant in conditions like viral hemorrhagic fevers such as those caused by hantavirus which lead to fever and thrombocytopenia. It plays a role in diseases by contributing to viral pathogenesis and influencing disease severity. The interaction of nucleoprotein with other proteins such as glycoproteins found in the viral envelope highlights its importance in the infectious cycle and its potential as a target for therapeutic interventions and vaccine development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Encapsidates the genome, protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC). Serves as template for viral transcription and replication. The increased presence of protein N in host cell does not seem to trigger the switch from transcription to replication as observed in other negative strain RNA viruses. Through the interaction with host IKBKE, strongly inhibits the phosphorylation and nuclear translocation of host IRF3, a protein involved in interferon activation pathway, leading to the inhibition of interferon-beta and IRF3-dependent promoters activation. Encodes also a functional 3'-5' exoribonuclease that degrades preferentially dsRNA substrates and thereby participates in the suppression of interferon induction.
See full target information N
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Product promise

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