Rabbit Polyclonal SHMT1 antibody. Suitable for WB and reacts with Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Lassa virus Josiah GPC.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Lassa virus Josiah GPC. The exact immunogen used to generate this antibody is proprietary information. Database link P08669
Reactivity data
Select an application| WB | |
|---|---|
| Synthetic peptide | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 0.1 µg/mL | Notes - |
Target data
Function
Stable signal peptide. Functions as a cleaved signal peptide that is retained as the third component of the GP complex (GP-C) (PubMed:35173332). Helps to stabilize the spike complex in its native conformation (PubMed:35173332). The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of G1 and G2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion (PubMed:14555961). Glycoprotein G1. Forms the virion spikes together with glycoprotein G2 (PubMed:26849049, PubMed:35173332). The glycoprotein spike trimers are connected to the underlying matrix (PubMed:26849049). Interacts with the host receptor. Mediates virus attachment to the host primary receptor alpha-dystroglycan DAG1 (alpha-DG) at the cell surface (PubMed:11967329, PubMed:27147735). This attachment induces virion internalization apparently through macropinocytosis (Probable) (PubMed:27147735). Following endocytosis, there is a pH-dependent switch from binding DAG1 to the host lysosomal receptor LAMP1 (PubMed:24970085, PubMed:27605678, PubMed:28448640). This latter binding triggers the dissociation of GP1, exposing the fusion subunit, GP2, such that fusion can occur (PubMed:24970085). Down-modulates host DAG1 (PubMed:17761532). Glycoprotein G2. Forms the virion spikes together with glycoprotein G1 (PubMed:26849049, PubMed:35173332). The glycoprotein spike trimers are connected to the underlying matrix (PubMed:26849049). Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced by acidification (PubMed:31004664).
Alternative names
GP-C, GPC, Pre-glycoprotein polyprotein GP complex, Pre-GP-C
Recommended products
Rabbit Polyclonal SHMT1 antibody. Suitable for WB and reacts with Synthetic peptide samples. Immunogen corresponding to Synthetic Peptide within Lassa virus Josiah GPC.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Lassa virus Josiah GPC. The exact immunogen used to generate this antibody is proprietary information. Database link P08669
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Lassa virus glycoprotein (LASV GP) functions as an important element in virus entry. This glycoprotein consists of two subunits GP1 and GP2 which undergo cleavage for activation and have a combined molecular mass of approximately 75 kDa. Of the two GP1 facilitates viral attachment to host cells while GP2 mediates membrane fusion. LASV GP primarily expresses on the surface of Lassa virus particles and infected host cells playing an important role in initiating infection.
- Biological function summary
The Lassa virus glycoprotein complex facilitates the entry and replication of the virus inside host cells. It requires interaction between GP1 and host cell receptors like alpha-dystroglycan to initiate virus attachment. GP2's fusogenic activity then enables fusion of viral and host membranes allowing viral RNA entry into the host cell cytoplasm. The glycoprotein complex drives viral infectivity and pathogenicity supporting the cross-species transmission of the virus.
- Pathways
LASV GP engages the endocytic pathway to deliver viral genetic material into host cells. This process involves the endocytosis and trafficking of virus-receptor complexes to late endosomes. It also coordinates with cellular proteins like cathepsin L for the cleavage necessary to activate membrane fusion. Such interactions with endocytic components highlight LASV GP's centrality in the viral life cycle and host-pathogen interactions.
- Associated diseases and disorders
LASV GP constitutes a fundamental role in Lassa fever pathogenesis. Lassa fever an acute viral hemorrhagic illness manifests after LASV GP mediates effective viral entry and replication in host tissues. The glycoprotein's interaction with host factors like alpha-dystroglycan marks it as a target in therapeutic strategies and vaccine development. The viral glycoprotein's influence on immune evasion and disease severity highlights its significance in the context of Lassa fever research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-LASV GP antibody (ab190655)
All lanes: Western blot - Anti-LASV GP antibody (ab190655) at 0.1 µg/mL
Lane 1: Recombinant Lassa virus glycoprotein at 0.05 µg
Lane 2: Recombinant Lassa virus glycoprotein at 0.1 µg
SecondaryAll lanes: Anti-rabbit IgG-HRP conjugate and TMB membrane substrate
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 60 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com