AB2507

Anti-LAT antibody

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(5 Publications)

Rabbit Polyclonal LAT antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human LAT aa 1-50.

View Alternative Names

Linker for activation of T-cells family member 1, 36 kDa phosphotyrosine adapter protein, p36-38, pp36, LAT

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Western blot - Anti-LAT antibody (AB2507)

Antibody recognizes approximately 36 KD size protein and its degradation products in mouse normal tissues. However, antibody specifically reacts with a approximately 46 KD protein in human tumor cell lines Jurkatt and K562. This immunoreactive 46 KD protein appears to be more stable than 36 KD immunoreactive protein observed in normal mouse tissue. (Lane 1, Human T cell line Jurkatt. Lane 2, Mouse Lung Lane 3, Human cell line K562 Lane 4, Mouse Heart).
Antibody recognizes approximately 36 KD size protein and its degradation products in mouse normal tissues. However, antibody specifically reacts with a approximately 46 KD protein in human tumor cell lines Jurkatt and K562. This immunoreactive 46 KD protein appears to be more stable than 36 KD immunoreactive protein observed in normal mouse tissue. (Lane 1, Human T cell line Jurkatt. Lane 2, Mouse Lung Lane 3, Human cell line K562 Lane 4, Mouse Heart).

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Western blot - Anti-LAT antibody (ab2507)

Predicted band size: 28 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human LAT aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O43561

Specificity

Binds to a 36-40 Kd protein in normal mouse lung and heart tissue. A larger size band of approx. 40-45 Kd is seen in human tumour cell lines.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.1% Sodium azide Constituents: 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAT or Linker for Activation of T cells also known as LAT protein is a transmembrane adaptor protein with an approximate molecular mass of 36-38 kDa. This protein is expressed predominantly on T cells and other cells of the lymphoid lineage. LAT is localized to the plasma membrane and contains multiple tyrosine residues which are critical for its function in signal transduction. Its phosphorylation leads to binding of various signaling molecules enabling signal propagation.

Biological function summary

LAT plays an important role in the immune response by facilitating the assembly of signaling complexes important for T cell activation. Upon antigen stimulation LAT becomes phosphorylated mainly at tyrosine residues allowing proteins like Grb2 and PLCγ1 to form a functional signalosome complex. This complex formation is necessary for downstream signaling events that contribute to T cell proliferation differentiation and cytokine production thereby maintaining immune homeostasis and facilitating adaptive immune responses.

Pathways

LAT operates primarily within the T cell receptor (TCR) signaling pathway. The phosphorylation of LAT by ZAP-70 another important signaling protein enables its role as a scaffold bringing together other molecules for signal transduction. It is closely related to proteins like SLP-76 and GADS both of which are essential for the full activation of T cells. These interactions link LAT to broader signaling cascades such as the MAPK and calcium signaling pathways which are essential for mounting an immune response.

LAT dysfunction is linked to both autoimmune diseases and immunodeficiencies. Defective LAT expression or function can lead to conditions like severe combined immunodeficiency (SCID) due to impaired T cell signaling. Additionally abnormal LAT activity may contribute to autoimmune disorders like systemic lupus erythematosus (SLE) where an overactive immune response is present. Proteins such as LAT can interact with others involved in these conditions including PLCγ1 highlighting its central role in both healthy and diseased states of immune regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development (PubMed : 23514740, PubMed : 25907557). Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

See full target information LAT

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:8959-8974 PubMed32469452

2020

PTPN22 interacts with EB1 to regulate T-cell receptor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaonan Zhang,Yang Yu,Bin Bai,Tao Wang,Jiahui Zhao,Na Zhang,Yanjiao Zhao,Xipeng Wang,Bing Wang

Diabetes 62:2048-58 PubMed23349496

2013

TSG-6 produced by hMSCs delays the onset of autoimmune diabetes by suppressing Th1 development and enhancing tolerogenicity.

Applications

Species

Unspecified reactive species

Daniel J Kota,Lindsey L Wiggins,Nara Yoon,Ryang Hwa Lee

Proceedings of the National Academy of Sciences of the United States of America 109:E3-12 PubMed22135465

2011

Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival.

Applications

Species

Mouse

Julie Zikherman,Kristin Doan,Ramya Parameswaran,William Raschke,Arthur Weiss

The Journal of biological chemistry 283:15419-30 PubMed18378687

2008

Inhibition of ZAP-70 kinase activity via an analog-sensitive allele blocks T cell receptor and CD28 superagonist signaling.

Applications

Species

Human

Susan E Levin,Chao Zhang,Theresa A Kadlecek,Kevan M Shokat,Arthur Weiss

The Journal of biological chemistry 281:26391-9 PubMed16818493

2006

Microtubules and actin microfilaments regulate lipid raft/caveolae localization of adenylyl cyclase signaling components.

Applications

Unspecified application

Species

Unspecified reactive species

Brian P Head,Hemal H Patel,David M Roth,Fiona Murray,James S Swaney,Ingrid R Niesman,Marilyn G Farquhar,Paul A Insel

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