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AB32070

Anti-LAT antibody [Y109]

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(3 Publications)

Rabbit Recombinant Monoclonal LAT antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Linker for activation of T-cells family member 1, 36 kDa phosphotyrosine adapter protein, p36-38, pp36, LAT

3 Images
Flow Cytometry (Intracellular) - Anti-LAT antibody [Y109] (AB32070)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-LAT antibody [Y109] (AB32070)

Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling LAT with purified ab32070 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT antibody [Y109] (AB32070)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT antibody [Y109] (AB32070)

ab32070 at a dilution of 1/100 staining LAT in paraffin embedded human lymph node tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-LAT antibody [Y109] (AB32070)
  • WB

Unknown

Western blot - Anti-LAT antibody [Y109] (AB32070)

All lanes:

Western blot - Anti-LAT antibody [Y109] (ab32070) at 1/500 dilution

All lanes:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Predicted band size: 28 kDa

Observed band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y109

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific for LAT. It detects the long and short forms of LAT.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAT or Linker for Activation of T cells also known as LAT protein is a transmembrane adaptor protein with an approximate molecular mass of 36-38 kDa. This protein is expressed predominantly on T cells and other cells of the lymphoid lineage. LAT is localized to the plasma membrane and contains multiple tyrosine residues which are critical for its function in signal transduction. Its phosphorylation leads to binding of various signaling molecules enabling signal propagation.
Biological function summary

LAT plays an important role in the immune response by facilitating the assembly of signaling complexes important for T cell activation. Upon antigen stimulation LAT becomes phosphorylated mainly at tyrosine residues allowing proteins like Grb2 and PLCγ1 to form a functional signalosome complex. This complex formation is necessary for downstream signaling events that contribute to T cell proliferation differentiation and cytokine production thereby maintaining immune homeostasis and facilitating adaptive immune responses.

Pathways

LAT operates primarily within the T cell receptor (TCR) signaling pathway. The phosphorylation of LAT by ZAP-70 another important signaling protein enables its role as a scaffold bringing together other molecules for signal transduction. It is closely related to proteins like SLP-76 and GADS both of which are essential for the full activation of T cells. These interactions link LAT to broader signaling cascades such as the MAPK and calcium signaling pathways which are essential for mounting an immune response.

LAT dysfunction is linked to both autoimmune diseases and immunodeficiencies. Defective LAT expression or function can lead to conditions like severe combined immunodeficiency (SCID) due to impaired T cell signaling. Additionally abnormal LAT activity may contribute to autoimmune disorders like systemic lupus erythematosus (SLE) where an overactive immune response is present. Proteins such as LAT can interact with others involved in these conditions including PLCγ1 highlighting its central role in both healthy and diseased states of immune regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development (PubMed : 23514740, PubMed : 25907557). Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
See full target information LAT
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

British journal of pharmacology 182:3923-3951 PubMed40344208

2025

A mitochondria-targeting and G-quadruplex structure-binding ligand inducing calcium overload and ferroptosis in human cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Xin Zheng,Wei Long,Yao-Xun Zeng,Meng-Ting She,Yingying Zheng,Wen-De Zheng,Ya-Kun Wang,Ka-Hin Chan,Alan Siu-Lun Leung,Chun-Ming Chan,Yu-Jing Lu,Wing-Leung Wong

European journal of haematology 109:513-518 PubMed35871391

2022

Loss of LEF-1 expression as a diagnostic indicator for extranodal NK/T-cell lymphoma: An immunohistochemical study of 88 cases.

Applications

Unspecified application

Species

Unspecified reactive species

Cen Qiu,Guangye Du

International journal of molecular sciences 16:11196-212 PubMed25993291

2015

Immunohistochemical analysis of the natural killer cell cytotoxicity pathway in human abdominal aortic aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Hinterseher,Charles M Schworer,John H Lillvis,Elizabeth Stahl,Robert Erdman,Zoran Gatalica,Gerard Tromp,Helena Kuivaniemi
View all publications
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