AB73205

Anti-LAT (phospho Y171) antibody

Name: Anti-LAT (phospho Y171) antibody (ab73205) | Abcam
Brand: Abcam Limited
SKU: AB73205
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal LAT phospho Y171 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human LAT phospho Y171.

View Alternative Names

Linker for activation of T-cells family member 1, 36 kDa phosphotyrosine adapter protein, p36-38, pp36, LAT

1 Images

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Western blot - Anti-LAT (phospho Y171) antibody (AB73205)

All lanes:

Western blot - Anti-LAT (phospho Y171) antibody (ab73205) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells treated with UV (5 min) at 30 µg

Lane 2:

Extracts from Jurkat cells treated with UV (5 min) at 30 µg with immunising phosphopeptide

Predicted band size: 28 kDa

Observed band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human LAT phospho Y171. The exact immunogen used to generate this antibody is proprietary information.

O43561

Specificity

Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab73205 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAT or Linker for Activation of T cells also known as LAT protein is a transmembrane adaptor protein with an approximate molecular mass of 36-38 kDa. This protein is expressed predominantly on T cells and other cells of the lymphoid lineage. LAT is localized to the plasma membrane and contains multiple tyrosine residues which are critical for its function in signal transduction. Its phosphorylation leads to binding of various signaling molecules enabling signal propagation.

Biological function summary

LAT plays an important role in the immune response by facilitating the assembly of signaling complexes important for T cell activation. Upon antigen stimulation LAT becomes phosphorylated mainly at tyrosine residues allowing proteins like Grb2 and PLCγ1 to form a functional signalosome complex. This complex formation is necessary for downstream signaling events that contribute to T cell proliferation differentiation and cytokine production thereby maintaining immune homeostasis and facilitating adaptive immune responses.

Pathways

LAT operates primarily within the T cell receptor (TCR) signaling pathway. The phosphorylation of LAT by ZAP-70 another important signaling protein enables its role as a scaffold bringing together other molecules for signal transduction. It is closely related to proteins like SLP-76 and GADS both of which are essential for the full activation of T cells. These interactions link LAT to broader signaling cascades such as the MAPK and calcium signaling pathways which are essential for mounting an immune response.

LAT dysfunction is linked to both autoimmune diseases and immunodeficiencies. Defective LAT expression or function can lead to conditions like severe combined immunodeficiency (SCID) due to impaired T cell signaling. Additionally abnormal LAT activity may contribute to autoimmune disorders like systemic lupus erythematosus (SLE) where an overactive immune response is present. Proteins such as LAT can interact with others involved in these conditions including PLCγ1 highlighting its central role in both healthy and diseased states of immune regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development (PubMed : 23514740, PubMed : 25907557). Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

See full target information LAT phospho Y171

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

European journal of immunology 44:3741-6 PubMed25251667

2014

NF-κB is crucial in proximal T-cell signaling for calcium influx and NFAT activation.

Applications

Unspecified application

Species

Unspecified reactive species

Crystina C Bronk,Sean Yoder,Emily L Hopewell,Shengyu Yang,Esteban Celis,Xue-Zhong Yu,Amer A Beg

Journal of immunology (Baltimore, Md. : 1950) 186:784-90 PubMed21149602

2010

Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-γ1 phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory Driessens,Yan Zheng,Frederick Locke,Judy L Cannon,Fotini Gounari,Thomas F Gajewski

View all publications

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