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AB68139

Anti-LAT (phospho Y200) antibody [EP983(2)Y]

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(9 Publications)

Rabbit Recombinant Monoclonal LAT phospho Y200 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.

View Alternative Names

Linker for activation of T-cells family member 1, 36 kDa phosphotyrosine adapter protein, p36-38, pp36, LAT

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (AB68139)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (AB68139)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat cells labelling LAT with ab68139 at 1/250 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing no staining in Jurkat cells and positive staining in Jurkat cells treated with pervanadate 1mM for 30 mins. ab195889 AlexaFluor® 594-conjugated Anti-alpha Tubulin was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (AB68139)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (AB68139)

Flow cytometric analysis of 4% paraformaldehyde fixed bLA permeabilized Jurkat cells labelling LAT with ab68139 at 1 : 2000 dilution (Red) compared with an isotype control (Black) and an unlabelled control (Cell without incubation with primary antibody and secondary antibody (Blue)). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (AB68139)
  • WB

Supplier Data

Western blot - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (AB68139)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-LAT (phospho Y200) antibody [EP983(2)Y] (ab68139) at 1/10000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Lane 2:

Jurkat treated with 1mM pervanadate for 30 min whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 1 kDa

false

Exposure time: 7s

  • Carrier free

    Anti-LAT (phospho Y200) antibody [EP983(2)Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP983(2)Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody only detects LAT phosphorylated on Tyrosine 200 of long isoform and Tyrosine 171 of short isoform.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2000", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAT or Linker for Activation of T cells also known as LAT protein is a transmembrane adaptor protein with an approximate molecular mass of 36-38 kDa. This protein is expressed predominantly on T cells and other cells of the lymphoid lineage. LAT is localized to the plasma membrane and contains multiple tyrosine residues which are critical for its function in signal transduction. Its phosphorylation leads to binding of various signaling molecules enabling signal propagation.
Biological function summary

LAT plays an important role in the immune response by facilitating the assembly of signaling complexes important for T cell activation. Upon antigen stimulation LAT becomes phosphorylated mainly at tyrosine residues allowing proteins like Grb2 and PLCγ1 to form a functional signalosome complex. This complex formation is necessary for downstream signaling events that contribute to T cell proliferation differentiation and cytokine production thereby maintaining immune homeostasis and facilitating adaptive immune responses.

Pathways

LAT operates primarily within the T cell receptor (TCR) signaling pathway. The phosphorylation of LAT by ZAP-70 another important signaling protein enables its role as a scaffold bringing together other molecules for signal transduction. It is closely related to proteins like SLP-76 and GADS both of which are essential for the full activation of T cells. These interactions link LAT to broader signaling cascades such as the MAPK and calcium signaling pathways which are essential for mounting an immune response.

LAT dysfunction is linked to both autoimmune diseases and immunodeficiencies. Defective LAT expression or function can lead to conditions like severe combined immunodeficiency (SCID) due to impaired T cell signaling. Additionally abnormal LAT activity may contribute to autoimmune disorders like systemic lupus erythematosus (SLE) where an overactive immune response is present. Proteins such as LAT can interact with others involved in these conditions including PLCγ1 highlighting its central role in both healthy and diseased states of immune regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development (PubMed : 23514740, PubMed : 25907557). Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
See full target information LAT phospho Y200
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Faraday discussions 259:45-59 PubMed40351229

2025

Immune cell activation produces locally scrambled foci of plasma membrane lipids.

Applications

Unspecified application

Species

Unspecified reactive species

Daryna Sputay,Milka Doktorova,Sze Ham Chan,Emma Han Guo,Hong-Yin Wang,Joseph H Lorent,Ilya Levental,Kandice R Levental

Journal of thrombosis and haemostasis : JTH 20:2617-2631 PubMed35894121

2022

Anti-GPVI nanobody blocks collagen- and atherosclerotic plaque-induced GPVI clustering, signaling, and thrombus formation.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie J Jooss,Christopher W Smith,Alexandre Slater,Samantha J Montague,Ying Di,Christopher O'Shea,Mark R Thomas,Yvonne M C Henskens,Johan W M Heemskerk,Steve P Watson,Natalie S Poulter

Cells 10: PubMed34685597

2021

1,8-Cineole Affects Agonists-Induced Platelet Activation, Thrombus Formation and Haemostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kahdr A Alatawi,Divyashree Ravishankar,Pabitra H Patra,Alexander P Bye,Alexander R Stainer,Ketan Patel,Darius Widera,Sakthivel Vaiyapuri

Science signaling 13: PubMed32753479

2020

The collagen receptor glycoprotein VI promotes platelet-mediated aggregation of β-amyloid.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Donner,Laura Mara Toska,Irena Krüger,Sandra Gröniger,Ruben Barroso,Alice Burleigh,Diego Mezzano,Susanne Pfeiler,Malte Kelm,Norbert Gerdes,Steve P Watson,Yi Sun,Margitta Elvers

Scientific reports 9:17210 PubMed31748641

2019

Non-genomic effects of the Pregnane X Receptor negatively regulate platelet functions, thrombosis and haemostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Gagan D Flora,Khaled A Sahli,Parvathy Sasikumar,Lisa-Marie Holbrook,Alexander R Stainer,Sarah K AlOuda,Marilena Crescente,Tanya Sage,Amanda J Unsworth,Jonathan M Gibbins

Journal of thrombosis and haemostasis : JTH 18:485-496 PubMed31680418

2019

Interspecies differences in protein expression do not impact the spatiotemporal regulation of glycoprotein VI mediated activation.

Applications

Unspecified application

Species

Unspecified reactive species

Joanne L Dunster,Amanda J Unsworth,Alexander P Bye,Elizabeth J Haining,Marcin A Sowa,Ying Di,Tanya Sage,Chiara Pallini,Jeremy A Pike,Alexander T Hardy,Bernhard Nieswandt,Ángel García,Steve P Watson,Natalie S Poulter,Jonathan M Gibbins,Alice Y Pollitt

Scientific reports 8:3494 PubMed29472624

2018

Citalopram inhibits platelet function independently of SERT-mediated 5-HT transport.

Applications

Unspecified application

Species

Unspecified reactive species

Harvey G Roweth,Ruoling Yan,Nader H Bedwani,Alisha Chauhan,Nicole Fowler,Alice H Watson,Jean-Daniel Malcor,Stewart O Sage,Gavin E Jarvis

Platelets 28:629-642 PubMed28032533

2016

Tetraspanin Tspan9 regulates platelet collagen receptor GPVI lateral diffusion and activation.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth J Haining,Alexandra L Matthews,Peter J Noy,Hanna M Romanska,Helen J Harris,Jeremy Pike,Martina Morowski,Rebecca L Gavin,Jing Yang,Pierre-Emmanuel Milhiet,Fedor Berditchevski,Bernhard Nieswandt,Natalie S Poulter,Steve P Watson,Michael G Tomlinson

Journal of immunology (Baltimore, Md. : 1950) 186:931-9 PubMed21160038

2010

Lipid raft assembly and Lck recruitment in TRAIL costimulation mediates NF-κB activation and T cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Chia Huang,Hwei-Fang Tsai,Horng-Tay Tzeng,Hsiu-Jung Liao,Ping-Ning Hsu
View all publications
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Product promise

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