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AB317207

Anti-LAT1 / SLC7A5 antibody [HL2353]

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Rabbit Monoclonal SLC7A5/LAT1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SLC7A5.

View Alternative Names

CD98LC, LAT1, MPE16, SLC7A5, Large neutral amino acids transporter small subunit 1, 4F2 light chain, CD98 light chain, Integral membrane protein E16, L-type amino acid transporter 1, Solute carrier family 7 member 5, y+ system cationic amino acid transporter, 4F2 LC, 4F2LC, E16, hLAT1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)

ab317207 detects LAT1 / SLC7A5 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded human lung cancer. LAT1 / SLC7A5 stained by ab317207 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)

ab317207 detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis. Sample : MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green : LAT1 / SLC7A5 stained by ab317207 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)
  • WB

Supplier Data

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)

Boiled and unboiled HeLa whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317207 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (WCE : whole cell extract; ME : membrane extract)

All lanes:

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (ab317207) at 1/1000 dilution

Lane 1:

Boiled HeLa whole cell extracts at 30 µg

Lane 2:

Boiled HeLa membrane extracts at 30 µg

Lane 3:

Unboiled HeLa whole cell extracts at 30 µg

Lane 4:

Unboiled HeLa membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)
  • WB

Supplier Data

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)

Non-transfected (–) and transfected (+) MCF-7 whole cell extract (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317207 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (ab317207) at 1/1000 dilution

Lane 1:

Non-transfected (–) MCF-7 whole cell extract at 30 µg

Lane 2:

SLC7A5 shRNA transfected (+) MCF-7 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)
  • WB

Supplier Data

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317207 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (ab317207) at 1/1000 dilution

Lane 1:

MCF-7 whole cell extracts at 30 µg

Lane 2:

A549 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)
  • WB

Supplier Data

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (AB317207)

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317207 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-LAT1 / SLC7A5 antibody [HL2353] (ab317207) at 1/1000 dilution

Lane 1:

Mouse ovary tissue extract at 50 µg

Lane 2:

Rat ovary tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2353

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human SLC7A5.

Q01650

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLC7A5/LAT1 or LAT1 transporter also known as the large neutral amino acid transporter small subunit 1 plays an important role in facilitating the transport of large neutral amino acids across cell membranes. LAT1 is a heterodimer with a heavy chain known usually as SLC3A2 forming a functional transporter which is around 40-50 kDa in mass. LAT1 is highly expressed in tissues with high metabolic rates like the brain placenta and some cancer cells making it important for nutrient transport in these areas.
Biological function summary

The LAT1 transporter mediates the uptake of essential amino acids which are critical for protein synthesis and cellular metabolism. It functions as part of the LAT1-SLC3A2 heterodimer complex ensuring effective transport across the blood-brain barrier and into other metabolically active tissues. This activity supports cellular growth and proliferation underlining its importance in rapidly dividing cells such as in cancer.

Pathways

LAT1 plays a critical role in the mTOR signaling pathway which is central to regulating cell growth and metabolism in response to nutrient availability. LAT1's transport function influences the activation of mTORC1 by ensuring a steady supply of leucine an amino acid that activates this complex. LAT1 is also integral to the cross-pathway interaction with the insulin signaling pathway enhancing nutrient-dependent growth signals in conjunction with other transporters.

Abnormal LAT1 function links closely with cancer and neurological disorders. Its overexpression often marks various tumors where it supports cancer cell survival by sustaining amino acid supply often in conjunction with increased SLC3A2 expression. In neurological contexts LAT1 impairments can contribute to disorders involving neurotransmitter imbalances as it participates in amino acid transport critical for neurotransmitter synthesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The heterodimer with SLC3A2 functions as a sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, leucine, histidine, methionine, tryptophan, valine, isoleucine and alanine (PubMed : 10049700, PubMed : 10574970, PubMed : 11557028, PubMed : 11564694, PubMed : 12117417, PubMed : 12225859, PubMed : 15769744, PubMed : 18262359, PubMed : 25998567, PubMed : 30867591, PubMed : 9751058). The heterodimer with SLC3A2 mediates the uptake of L-DOPA (By similarity). Functions as an amino acid exchanger (PubMed : 11557028, PubMed : 12117417, PubMed : 12225859, PubMed : 30867591). May play a role in the transport of L-DOPA across the blood-brain barrier (By similarity). May act as the major transporter of tyrosine in fibroblasts (Probable). May mediate blood-to-retina L-leucine transport across the inner blood-retinal barrier (By similarity). Can mediate the transport of thyroid hormones diiodothyronine (T2), triiodothyronine (T3) and thyroxine (T4) across the cell membrane (PubMed : 11564694). When associated with LAPTM4B, the heterodimer formed by SLC3A2 and SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation (PubMed : 25998567). Involved in the uptake of toxic methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes (PubMed : 12117417). Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the membrane (PubMed : 15769744).. (Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis.
See full target information SLC7A5
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