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AB75610

Anti-LAT2 antibody

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(1 Review)

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(11 Publications)

Rabbit Polyclonal LAT2 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 11 publications.

View Alternative Names

LAT2, SLC7A8, Large neutral amino acids transporter small subunit 2, L-type amino acid transporter 2, Solute carrier family 7 member 8, hLAT2

3 Images
Western blot - Anti-LAT2 antibody (AB75610)
  • WB

Unknown

Western blot - Anti-LAT2 antibody (AB75610)

All lanes:

Western blot - Anti-LAT2 antibody (ab75610) at 1 µg/mL

Lane 1:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 2:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 58 kDa

Observed band size: 52 kDa,58 kDa,85 kDa

true

Exposure time: 20min

Western blot - Anti-LAT2 antibody (AB75610)
  • WB

Unknown

Western blot - Anti-LAT2 antibody (AB75610)

All lanes:

Western blot - Anti-LAT2 antibody (ab75610) at 1/1 dilution

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Kidney (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 58 kDa

Observed band size: 65 kDa

true

Exposure time: 2min

Western blot - Anti-LAT2 antibody (AB75610)
  • WB

CiteAb

Western blot - Anti-LAT2 antibody (AB75610)

LAT2 western blot using anti-LAT2 antibody ab75610. Publication image and figure legend from McIntyre, K. R., Vincent, K. M. M., et al., 2020, Sci Rep, PubMed 33004923.

ab75610 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab75610 please see the product overview.

Expression of glutamine [LAT1 (a), LAT2 (b), SNAT5 (c)] and glutamate [EAAT1 (d), EAAT2 (e)] transporter proteins in membrane-enriched placental homogenates from AGA (n = 7–11) and FGR (n = 8–10) infants. Densitometric analysis is expressed as a ratio of β-tubulin signal. Line denotes median, *** p < 0.001, * p < 0.05 : Wilcoxon signed-rank test.

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAT2 also known as Linker for Activation of T cells 2 is a cytoplasmic adaptor protein with a molecular mass of approximately 38 kDa. LAT2 plays an important role in intracellular signaling and is widely expressed in immune system cells especially T cells mast cells and platelets. This protein functions as a scaffold providing binding sites for several signaling molecules involved in immune cell activation.
Biological function summary

LAT2 contributes significantly to immune system responses. It forms complexes with proteins such as SLP-76 to facilitate signal transduction. LAT2 becomes phosphorylated upon activation which recruits multiple effector molecules and serves as a platform for further signaling processes. This mechanism enhances the transmission of activation signals that lead to immune cell proliferation and differentiation.

Pathways

Proteins interacting with LAT2 participate in the T cell receptor (TCR) signaling pathway and FcεRI signaling in mast cells. LAT2 anchors important signaling molecules within these pathways including ZAP-70 and PLCγ1 which further propagate the signal cascade necessary for immune responses. The LAT2-SLP-76 complex helps coordinate the assembly of various intracellular signaling proteins in these pathways which is vital for effective cellular functions.

Defects or alterations in LAT2 function can result in immune system-related conditions such as immunodeficiencies and autoimmune disorders. Abnormal LAT2 signaling is linked to diseases like systemic lupus erythematosus where T cell signaling becomes dysregulated. Further LAT2 interacts with proteins such as Syk involved in other immune disorders highlighting its importance in maintaining immune system balance.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Associates with SLC3A2 to form a functional heterodimeric complex that translocates small and large neutral amino acids with broad specificity and a stoichiometry of 1 : 1. Functions as amino acid antiporter mediating the influx of extracellular essential amino acids mainly in exchange with the efflux of highly concentrated intracellular amino acids (PubMed : 10391915, PubMed : 11311135, PubMed : 11847106, PubMed : 12716892, PubMed : 15081149, PubMed : 15918515, PubMed : 29355479, PubMed : 33298890, PubMed : 34848541). Has relatively symmetrical selectivities but strongly asymmetrical substrate affinities at both the intracellular and extracellular sides of the transporter (PubMed : 11847106). This asymmetry allows SLC7A8 to regulate intracellular amino acid pools (mM concentrations) by exchange with external amino acids (uM concentration range), equilibrating the relative concentrations of different amino acids across the plasma membrane instead of mediating their net uptake (PubMed : 10391915, PubMed : 11847106). May play an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney (PubMed : 12716892). Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity (PubMed : 12117417). Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane (PubMed : 15769744). Imports the thyroid hormone diiodothyronine (T2) and to a smaller extent triiodothyronine (T3) but not rT 3 or thyroxine (T4) (By similarity). Mediates the uptake of L-DOPA (By similarity). May participate in auditory function (By similarity).
See full target information SLC7A8

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37298123

2023

Genetic Ablation of LAT1 Inhibits Growth of Liver Cancer Cells and Downregulates mTORC1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Sun-Yee Kim,Qunxiang Ong,Yilie Liao,Zhaobing Ding,Alicia Qian Ler Tan,Ler Ting Rachel Lim,Hui Min Tan,Siew Lan Lim,Qian Yi Lee,Weiping Han

Frontiers in medicine 9:827695 PubMed35602471

2022

Identification and Validation of Immune Molecular Subtypes and Immune Landscape Based on Colon Cancer Cohort.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqian Qi,Qian Zhang

Nature communications 13:1151 PubMed35241668

2022

Changes to gut amino acid transporters and microbiome associated with increased E/I ratio in Chd8 mouse model of ASD-like behavior.

Applications

Unspecified application

Species

Unspecified reactive species

You Yu,Bing Zhang,Peifeng Ji,Zhenqiang Zuo,Yongxi Huang,Ning Wang,Chang Liu,Shuang-Jiang Liu,Fangqing Zhao

Frontiers in oncology 11:683915 PubMed34976784

2021

Development and Validation of an Immune-Related Signature for the Prediction of Recurrence Risk of Patients With Laryngeal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Zhang,Xudong Zhao,Jin Wang,Wenyue Ji

JCI insight 6: PubMed34494553

2021

Genomic and epigenomic evolution of acquired resistance to combination therapy in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qingjie Min,Yan Wang,Qingnan Wu,Xianfeng Li,Huajing Teng,Jiawen Fan,Yiren Cao,Pingsheng Fan,Qimin Zhan

Scientific reports 10:16197 PubMed33004923

2020

Human placental uptake of glutamine and glutamate is reduced in fetal growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsty R McIntyre,Kirsty M M Vincent,Christina E Hayward,Xiaojia Li,Colin P Sibley,Michelle Desforges,Susan L Greenwood,Mark R Dilworth

Placenta 101:57-65 PubMed32927345

2020

Abnormalities of placental development and function are associated with the different fetal growth patterns of hypoplastic left heart syndrome and transposition of the great arteries.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Courtney,Weston Troja,Kathryn J Owens,Heather M Brockway,Andrea C Hinton,Robert B Hinton,James F Cnota,Helen N Jones

Nutrients 11: PubMed31752111

2019

Butyric Acid and Leucine Induce α-Defensin Secretion from Small Intestinal Paneth Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Akiko Takakuwa,Kiminori Nakamura,Mani Kikuchi,Rina Sugimoto,Shuya Ohira,Yuki Yokoi,Tokiyoshi Ayabe

The Journal of physiology 597:4975-4990 PubMed31400764

2019

Evidence of adaptation of maternofetal transport of glutamine relative to placental size in normal mice, and in those with fetal growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsty R McIntyre,Christina E Hayward,Colin P Sibley,Susan L Greenwood,Mark R Dilworth

BMC genomics 19:403 PubMed29843597

2018

Understanding the regulatory mechanisms of milk production using integrative transcriptomic and proteomic analyses: improving inefficient utilization of crop by-products as forage in dairy industry.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Dai,Quanjuan Wang,Fengqi Zhao,Jianxin Liu,Hongyun Liu
View all publications

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