Anti-LAT2 antibody
0
(1 Review)
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(11 Publications)
Rabbit Polyclonal LAT2 antibody. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 11 publications.
View Alternative Names
LAT2, SLC7A8, Large neutral amino acids transporter small subunit 2, L-type amino acid transporter 2, Solute carrier family 7 member 8, hLAT2
- WB
Unknown
Western blot - Anti-LAT2 antibody (AB75610)
All lanes:
Western blot - Anti-LAT2 antibody (ab75610) at 1 µg/mL
Lane 1:
Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg
Lane 2:
HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3:
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes:
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 58 kDa
Observed band size: 52 kDa,58 kDa,85 kDa
true
Exposure time: 20min
- WB
Unknown
Western blot - Anti-LAT2 antibody (AB75610)
All lanes:
Western blot - Anti-LAT2 antibody (ab75610) at 1/1 dilution
Lane 1:
Brain (Mouse) Tissue Lysate at 10 µg
Lane 2:
Kidney (Rat) Tissue Lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution
Predicted band size: 58 kDa
Observed band size: 65 kDa
true
Exposure time: 2min
- WB
CiteAb
Western blot - Anti-LAT2 antibody (AB75610)
LAT2 western blot using anti-LAT2 antibody ab75610. Publication image and figure legend from McIntyre, K. R., Vincent, K. M. M., et al., 2020, Sci Rep, PubMed 33004923.
ab75610 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab75610 please see the product overview.
Expression of glutamine [LAT1 (a), LAT2 (b), SNAT5 (c)] and glutamate [EAAT1 (d), EAAT2 (e)] transporter proteins in membrane-enriched placental homogenates from AGA (n = 7–11) and FGR (n = 8–10) infants. Densitometric analysis is expressed as a ratio of β-tubulin signal. Line denotes median, *** p < 0.001, * p < 0.05 : Wilcoxon signed-rank test.
false
Reactivity data
Properties and storage information
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Appropriate long-term storage conditions
Aliquoting information
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LAT2 contributes significantly to immune system responses. It forms complexes with proteins such as SLP-76 to facilitate signal transduction. LAT2 becomes phosphorylated upon activation which recruits multiple effector molecules and serves as a platform for further signaling processes. This mechanism enhances the transmission of activation signals that lead to immune cell proliferation and differentiation.
Pathways
Proteins interacting with LAT2 participate in the T cell receptor (TCR) signaling pathway and FcεRI signaling in mast cells. LAT2 anchors important signaling molecules within these pathways including ZAP-70 and PLCγ1 which further propagate the signal cascade necessary for immune responses. The LAT2-SLP-76 complex helps coordinate the assembly of various intracellular signaling proteins in these pathways which is vital for effective cellular functions.
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Target data
Publications (11)
Recent publications for all applications. Explore the full list and refine your search
International journal of molecular sciences 24: PubMed37298123
2023
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Frontiers in medicine 9:827695 PubMed35602471
2022
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Nature communications 13:1151 PubMed35241668
2022
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Frontiers in oncology 11:683915 PubMed34976784
2021
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JCI insight 6: PubMed34494553
2021
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Scientific reports 10:16197 PubMed33004923
2020
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Placenta 101:57-65 PubMed32927345
2020
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Nutrients 11: PubMed31752111
2019
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The Journal of physiology 597:4975-4990 PubMed31400764
2019
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BMC genomics 19:403 PubMed29843597
2018
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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