Mouse Monoclonal LAT2 antibody. Carrier free. Suitable for Protein Microarray ChIP, IHC-P, WB, ICC/IF and reacts with Purified native protein - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SLC7A8.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86228.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)")
![Western blot - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--western-blot-img7073.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)")
![Immunocytochemistry/ Immunofluorescence - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--immunocytochemistry-immunofluorescence-img129643.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129712.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123412.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human SLC7A8. Database link Q9UHI5
Reactivity data
Select an application| Protein Microarray ChIP | IHC-P | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Purified native protein - Human | Tested | Not recommended | Not recommended | Not recommended |
Protein Microarray ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Human | Dilution info - | Notes - |
Target data
Function
Associates with SLC3A2 to form a functional heterodimeric complex that translocates small and large neutral amino acids with broad specificity and a stoichiometry of 1:1. Functions as amino acid antiporter mediating the influx of extracellular essential amino acids mainly in exchange with the efflux of highly concentrated intracellular amino acids (PubMed:10391915, PubMed:11311135, PubMed:11847106, PubMed:12716892, PubMed:15081149, PubMed:15918515, PubMed:29355479, PubMed:33298890, PubMed:34848541). Has relatively symmetrical selectivities but strongly asymmetrical substrate affinities at both the intracellular and extracellular sides of the transporter (PubMed:11847106). This asymmetry allows SLC7A8 to regulate intracellular amino acid pools (mM concentrations) by exchange with external amino acids (uM concentration range), equilibrating the relative concentrations of different amino acids across the plasma membrane instead of mediating their net uptake (PubMed:10391915, PubMed:11847106). May play an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney (PubMed:12716892). Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity (PubMed:12117417). Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane (PubMed:15769744). Imports the thyroid hormone diiodothyronine (T2) and to a smaller extent triiodothyronine (T3) but not rT 3 or thyroxine (T4) (By similarity). Mediates the uptake of L-DOPA (By similarity). May participate in auditory function (By similarity).
Alternative names
LAT2, SLC7A8, Large neutral amino acids transporter small subunit 2, L-type amino acid transporter 2, Solute carrier family 7 member 8, hLAT2
Recommended products
Mouse Monoclonal LAT2 antibody. Carrier free. Suitable for Protein Microarray ChIP, IHC-P, WB, ICC/IF and reacts with Purified native protein - Human, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SLC7A8.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.3
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human SLC7A8. Database link Q9UHI5
- Carrier free
- Yes
- Clone number
- UMAB70
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LAT2 also known as Linker for Activation of T cells 2 is a cytoplasmic adaptor protein with a molecular mass of approximately 38 kDa. LAT2 plays an important role in intracellular signaling and is widely expressed in immune system cells especially T cells mast cells and platelets. This protein functions as a scaffold providing binding sites for several signaling molecules involved in immune cell activation.
- Biological function summary
LAT2 contributes significantly to immune system responses. It forms complexes with proteins such as SLP-76 to facilitate signal transduction. LAT2 becomes phosphorylated upon activation which recruits multiple effector molecules and serves as a platform for further signaling processes. This mechanism enhances the transmission of activation signals that lead to immune cell proliferation and differentiation.
- Pathways
Proteins interacting with LAT2 participate in the T cell receptor (TCR) signaling pathway and FcεRI signaling in mast cells. LAT2 anchors important signaling molecules within these pathways including ZAP-70 and PLCγ1 which further propagate the signal cascade necessary for immune responses. The LAT2-SLP-76 complex helps coordinate the assembly of various intracellular signaling proteins in these pathways which is vital for effective cellular functions.
- Associated diseases and disorders
Defects or alterations in LAT2 function can result in immune system-related conditions such as immunodeficiencies and autoimmune disorders. Abnormal LAT2 signaling is linked to diseases like systemic lupus erythematosus where T cell signaling becomes dysregulated. Further LAT2 interacts with proteins such as Syk involved in other immune disorders highlighting its importance in maintaining immune system balance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img86228.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)
Paraffin embedded human normal colon tissue (left) and colorectal cancer tissue (right) stained for SLC7A8 using the same antibody clone in a different buffer formulation (PBS, BSA, glycerol, and sodium azide) at 1/100 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3mins.
![Western blot - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--western-blot-img7073.jpg "Western blot - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)")
Western blot - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)
All lanes: Western blot - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674) at 1/500 dilution
Lane 1: Human testis tissue lysates at 15 µg
Lane 2: Human omentum tissue lysate at 15 µg
Lane 3: Human uterus tissue lysate at 15 µg
Lane 4: Human breast tissue lysate at 15 µg
Lane 5: Human brain tissue lysate at 15 µg
Lane 6: Human liver tissue lysate at 15 µg
Lane 7: Human ovary tissue lysate at 15 µg
Lane 8: Human thyroid tissue lysate at 15 µg
Lane 9: Human colon tissue lysate at 15 µg
Lane 10: Human spleen tissue lysate at 15 µg
Predicted band size: 58 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--immunocytochemistry-immunofluorescence-img129643.jpg "Immunocytochemistry/ Immunofluorescence - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)")
Immunocytochemistry/ Immunofluorescence - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for SLC7A8 (green) using the same antibody clone in a different buffer formulation (PBS, BSA, glycerol, and sodium azide) at 1/50 dilution in ICC/IF. Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129712.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)
Paraffin embedded human normal pancreas tissue (left) and pancreatic cancer tissue (right) stained for SLC7A8 using the same antibody clone in a different buffer formulation (PBS, BSA, glycerol, and sodium azide) at 1/100 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123412.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)
Paraffin embedded human normal kidney tissue (left) and kidney carcinoma tissue (right) stained for SLC7A8 using the same antibody clone in a different buffer formulation (PBS, BSA, glycerol, and sodium azide) at 1/100 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3mins.
![Protein Microarray ChIP - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--protein-microarray-chip-img101346.jpg "Protein Microarray ChIP - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)")
Protein Microarray ChIP - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)
Overexpression protein microarray chip was immunostained with same antibody clone in a different buffer (PBS, BSA, glycerol, and sodium azide).
The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
![Protein Microarray ChIP - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674), expandable thumbnail](https://content.abcam.com/products/images/lat2-antibody-umab70-bsa-and-azide-free-ab273674--protein-microarray-chip-img101346.jpg/_jcr_content/renditions/webp-475.webp "Protein Microarray ChIP - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674)")
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