Anti-LAT2 antibody [UMAB70] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)

Paraffin embedded human normal colon tissue (left) and colorectal cancer tissue (right) stained for SLC7A8 using the same antibody clone in a different buffer formulation (PBS, BSA, glycerol, and sodium azide) at 1/100 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)

Paraffin embedded human normal kidney tissue (left) and kidney carcinoma tissue (right) stained for SLC7A8 using the same antibody clone in a different buffer formulation (PBS, BSA, glycerol, and sodium azide) at 1/100 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for SLC7A8 (green) using the same antibody clone in a different buffer formulation (PBS, BSA, glycerol, and sodium azide) at 1/50 dilution in ICC/IF. Actin filaments were labeled with Alexa Fluor^® 594 Phalloidin (red), and nuclear with DAPI (blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)

Paraffin embedded human normal pancreas tissue (left) and pancreatic cancer tissue (right) stained for SLC7A8 using the same antibody clone in a different buffer formulation (PBS, BSA, glycerol, and sodium azide) at 1/100 dilution in immunohistochemical analysis.

Heat-induced epitope retrieval was performed using 10 mM citric buffer, pH 6.0, 120°C for 3mins.

• WB

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Western blot - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)

All lanes:

Western blot - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (ab273674) at 1/500 dilution

Lane 1:

Human testis tissue lysates at 15 µg

Lane 2:

Human omentum tissue lysate at 15 µg

Lane 3:

Human uterus tissue lysate at 15 µg

Lane 4:

Human breast tissue lysate at 15 µg

Lane 5:

Human brain tissue lysate at 15 µg

Lane 6:

Human liver tissue lysate at 15 µg

Lane 7:

Human ovary tissue lysate at 15 µg

Lane 8:

Human thyroid tissue lysate at 15 µg

Lane 9:

Human colon tissue lysate at 15 µg

Lane 10:

Human spleen tissue lysate at 15 µg

Predicted band size: 58 kDa

false

• Protein Microarray ChIP

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Protein Microarray ChIP - Anti-LAT2 antibody [UMAB70] - BSA and Azide free (AB273674)

Overexpression protein microarray chip was immunostained with same antibody clone in a different buffer (PBS, BSA, glycerol, and sodium azide).

The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.