Rabbit Polyclonal LATS1/WARTS antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LATS1 aa 1-300.
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- Cell death discovery 9:3412023NRP1 transduces mechanical stress inhibition via LATS1/YAP in hypertrophic scars.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengzhi Li,Peng Wang,Jingting Li,Fei Zhou,Shixin Huang,Shaohai Qi,Bin ShuPubMed 37704618
- Cell death discovery 8:592022YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangfei Xue,Xiaoting Tian,Congcong Zhang,Yayou Miao,Yikun Wang,Yingxiu Peng,Shiyu Qiu,Hong Wang,Jiangtao Cui,Leiqun Cao,Fenyong Sun,Yongxia Qiao,Xiao ZhangPubMed 35149670
- Molecular therapy. Nucleic acids 25:116-1262021lncRNA H19 facilitates the proliferation and differentiation of human dental pulp stem cells via EZH2-dependent LATS1 methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Du,Xiaoming Shi,Aizhong GuanPubMed 34401209
- Journal of cancer research and clinical oncology 147:373-3912020The clinical relevance of the Hippo pathway in pancreatic ductal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard Drexler,Mirco Küchler,Kim C Wagner,Tim Reese,Bernd Feyerabend,Moritz Kleine,Karl J OldhaferPubMed 33098447
- EBioMedicine 56:1027952020SPOP promotes ubiquitination and degradation of LATS1 to enhance kidney cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLixia Wang et. al.PubMed 32460168
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LATS1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link O95835
Reactivity data
Select an application| IP | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Associated Products
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5 products for Alternative Product
Target data
Function
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed:28068668).
Alternative names
WARTS, LATS1, Serine/threonine-protein kinase LATS1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts
Recommended products
Rabbit Polyclonal LATS1/WARTS antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LATS1 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LATS1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link O95835
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
LATS1/WARTS also known as Large Tumor Suppressor 1 is a serine/threonine protein kinase with a molecular mass of approximately 125 kDa. Its expression is found in various tissue types including skin lung and liver. Mechanically LATS1/WARTS plays a critical role in regulating the cell cycle and maintaining genomic stability. This kinase is an essential part of the Hippo signaling pathway and contributes to controlling the size of organs by inhibiting cell proliferation and promoting apoptosis.
- Biological function summary
LATS1/WARTS functions as an important regulator of cellular growth and differentiation. It is part of the Hippo signaling complex which includes proteins like MST1/2 and YAP/TAZ. This complex helps maintain proper tissue homeostasis by modulating gene expression in response to cellular density and mechanical cues. In doing so LATS1/WARTS phosphorylates and inactivates downstream effectors YAP and TAZ preventing them from translocating to the nucleus where they could promote transcription of growth-promoting genes.
- Pathways
Scientists have found LATS1/WARTS to be integral to both the Hippo signaling pathway and the cell cycle regulation pathway. Within these pathways LATS1/WARTS interacts closely with proteins such as MOB1 and YAP mediating cellular responses to growth inhibitory signals. By controlling these interactions LATS1/WARTS helps coordinate cell proliferation and apoptosis which is critical for proper developmental processes and prevention of tumor growth.
- Associated diseases and disorders
Dysregulation of LATS1/WARTS function is associated with the development of cancers particularly in the liver and breast. Research also links LATS1/WARTS to neurodegenerative conditions by implicating its role in apoptosis and cell survival. When its kinase activity is compromised the regulatory balance maintained by the Hippo pathway is disrupted potentially leading to unchecked cell proliferation. Connections with proteins such as YAP and TAZ further illustrate its involvement in these pathological conditions showing its importance in maintaining cellular integrity and function.
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-LATS1/WARTS antibody (ab234820)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for LATS1/WARTS (green) using ab234820 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
Immunoprecipitation - Anti-LATS1/WARTS antibody (ab234820)
LATS1/WARTS was immunoprecipitated from 500 μg K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with 6 μg ab234820.
Lane 1: Rabbit control IgG IP (1 μg) in K562 whole cell lysate.
Lane 2: ab234820 IP in K562 whole cell lysate.
Lane 3: K562 whole cell lysate 10 μg (Input).For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
All lanes: Immunoprecipitation - Anti-LATS1/WARTS antibody (ab234820)
Predicted band size: 126 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (ab234820)
Paraffin-embedded human liver cancer tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (ab234820)
Paraffin-embedded human placenta tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (ab234820)
Paraffin-embedded human liver cancer tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.
Performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (ab234820)
Paraffin-embedded human adrenal gland tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.
Performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody.
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