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AB234820

Anti-LATS1/WARTS antibody

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(8 Publications)

Rabbit Polyclonal LATS1/WARTS antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human LATS1 aa 1-300.

View Alternative Names

WARTS, LATS1, Serine/threonine-protein kinase LATS1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (AB234820)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (AB234820)

Paraffin-embedded human liver cancer tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-LATS1/WARTS antibody (AB234820)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LATS1/WARTS antibody (AB234820)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for LATS1/WARTS (green) using ab234820 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (AB234820)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (AB234820)

Paraffin-embedded human adrenal gland tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.

Performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (AB234820)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (AB234820)

Paraffin-embedded human placenta tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (AB234820)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS1/WARTS antibody (AB234820)

Paraffin-embedded human liver cancer tissue stained for LATS1/WARTS using ab234820 at 1/100 dilution in immunohistochemical analysis.

Performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody.

Immunoprecipitation - Anti-LATS1/WARTS antibody (AB234820)
  • IP

Supplier Data

Immunoprecipitation - Anti-LATS1/WARTS antibody (AB234820)

LATS1/WARTS was immunoprecipitated from 500 μg K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with 6 μg ab234820.

Lane 1 : Rabbit control IgG IP (1 μg) in K562 whole cell lysate.
Lane 2 : ab234820 IP in K562 whole cell lysate.
Lane 3 : K562 whole cell lysate 10 μg (Input).

For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-LATS1/WARTS antibody (ab234820)

Predicted band size: 126 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, IP

applications

Immunogen

Recombinant Fragment Protein within Human LATS1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O95835

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/200 - 1/2000", "IP-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LATS1/WARTS also known as Large Tumor Suppressor 1 is a serine/threonine protein kinase with a molecular mass of approximately 125 kDa. Its expression is found in various tissue types including skin lung and liver. Mechanically LATS1/WARTS plays a critical role in regulating the cell cycle and maintaining genomic stability. This kinase is an essential part of the Hippo signaling pathway and contributes to controlling the size of organs by inhibiting cell proliferation and promoting apoptosis.
Biological function summary

LATS1/WARTS functions as an important regulator of cellular growth and differentiation. It is part of the Hippo signaling complex which includes proteins like MST1/2 and YAP/TAZ. This complex helps maintain proper tissue homeostasis by modulating gene expression in response to cellular density and mechanical cues. In doing so LATS1/WARTS phosphorylates and inactivates downstream effectors YAP and TAZ preventing them from translocating to the nucleus where they could promote transcription of growth-promoting genes.

Pathways

Scientists have found LATS1/WARTS to be integral to both the Hippo signaling pathway and the cell cycle regulation pathway. Within these pathways LATS1/WARTS interacts closely with proteins such as MOB1 and YAP mediating cellular responses to growth inhibitory signals. By controlling these interactions LATS1/WARTS helps coordinate cell proliferation and apoptosis which is critical for proper developmental processes and prevention of tumor growth.

Dysregulation of LATS1/WARTS function is associated with the development of cancers particularly in the liver and breast. Research also links LATS1/WARTS to neurodegenerative conditions by implicating its role in apoptosis and cell survival. When its kinase activity is compromised the regulatory balance maintained by the Hippo pathway is disrupted potentially leading to unchecked cell proliferation. Connections with proteins such as YAP and TAZ further illustrate its involvement in these pathological conditions showing its importance in maintaining cellular integrity and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed : 10518011, PubMed : 10831611, PubMed : 18158288, PubMed : 26437443, PubMed : 28068668). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed : 18158288, PubMed : 26437443, PubMed : 28068668). Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed : 18158288, PubMed : 26437443, PubMed : 28068668). Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint (PubMed : 15122335, PubMed : 19927127). Negatively regulates G2/M transition by down-regulating CDK1 kinase activity (PubMed : 9988268). Involved in the control of p53 expression (PubMed : 15122335). Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1 (PubMed : 15220930). May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed : 28068668). Acts as an activator of the NLRP3 inflammasome by mediating phosphorylation of 'Ser-265' of NLRP3 following NLRP3 palmitoylation, promoting NLRP3 activation by NEK7 (PubMed : 39173637).
See full target information LATS1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 25:2385172 PubMed39097779

2024

KLF7 enhances the invasion and migration of colorectal cancer cells via the miR-139-5p/TPD52 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Zhang,Zhihan Li,Jiaxu Han,Zhongtao Tian,Qingyu Meng,Wenbo Niu

Journal of cellular physiology 239:e31220 PubMed38372068

2024

mA-induced TRIB3 regulates Hippo pathway through interacting with LATS1 to promote the progression of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamei Wu,Tingzhuang Yi,Chenyi Zhuo,Duanduan Wang,Ming Zhang,Rui Hu,Dan Wu,Guoxin Hou,Yutong Xing

Cell death discovery 9:341 PubMed37704618

2023

NRP1 transduces mechanical stress inhibition via LATS1/YAP in hypertrophic scars.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhi Li,Peng Wang,Jingting Li,Fei Zhou,Shixin Huang,Shaohai Qi,Bin Shu

iScience 26:107617 PubMed37664594

2023

SHOX2 promotes prostate cancer proliferation and metastasis through disruption of the Hippo-YAP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Yang,Hualin Chen,Lin Ma,Jie Dong,Mengchao Wei,Xiaoqiang Xue,Yingjie Li,Zhaoheng Jin,Weifeng Xu,Zhigang Ji

Cell death discovery 8:59 PubMed35149670

2022

YAP ISGylation increases its stability and promotes its positive regulation on PPP by stimulating 6PGL transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangfei Xue,Xiaoting Tian,Congcong Zhang,Yayou Miao,Yikun Wang,Yingxiu Peng,Shiyu Qiu,Hong Wang,Jiangtao Cui,Leiqun Cao,Fenyong Sun,Yongxia Qiao,Xiao Zhang

Molecular therapy. Nucleic acids 25:116-126 PubMed34401209

2021

lncRNA H19 facilitates the proliferation and differentiation of human dental pulp stem cells via EZH2-dependent LATS1 methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Du,Xiaoming Shi,Aizhong Guan

Journal of cancer research and clinical oncology 147:373-391 PubMed33098447

2020

The clinical relevance of the Hippo pathway in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Richard Drexler,Mirco Küchler,Kim C Wagner,Tim Reese,Bernd Feyerabend,Moritz Kleine,Karl J Oldhafer

EBioMedicine 56:102795 PubMed32460168

2020

SPOP promotes ubiquitination and degradation of LATS1 to enhance kidney cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Wang,Min Lin,Man Chu,Yi Liu,Jia Ma,Youhua He,Zhi-Wei Wang
View all publications
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