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AB70561

Anti-LATS1/WARTS antibody

4

(1 Review)

|

(34 Publications)

Rabbit Polyclonal LATS1/WARTS antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 34 publications. Immunogen corresponding to Synthetic Peptide within Human LATS1 aa 50-100.

View Alternative Names

WARTS, LATS1, Serine/threonine-protein kinase LATS1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts

3 Images
Immunoprecipitation - Anti-LATS1/WARTS antibody (AB70561)
  • IP

Unknown

Immunoprecipitation - Anti-LATS1/WARTS antibody (AB70561)

Detection of human LATS1/WARTS by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg, 1/4 of immunoprecipitate loaded/lane), using ab70561 at 3 μg/mg of lysate (lane 1).
LATS1/WARTS was also immunoprecipitated with an other LATS1/WARTS antibody (lanes 2) and with ab70562 (lane 3) using 3 μg/mg lysate.
Lane 4 : Control IgG immunoprecipitate.
Subsequent Western blot detection of LATS1/WARTS was performed using ab70561 at 0.1 μg/ml.

All lanes:

Immunoprecipitation - Anti-LATS1/WARTS antibody (ab70561)

Predicted band size: 126 kDa

false

Western blot - Anti-LATS1/WARTS antibody (AB70561)
  • WB

Unknown

Western blot - Anti-LATS1/WARTS antibody (AB70561)

All lanes:

Western blot - Anti-LATS1/WARTS antibody (ab70561) at 0.02 µg/mL

Lane 1:

Whole cell lysate from Hela cells at 50 µg

Lane 2:

Whole cell lysate from Hela cells at 15 µg

Predicted band size: 126 kDa

Observed band size: 117 kDa,160 kDa,200 kDa,268 kDa,71 kDa

false

Western blot - Anti-LATS1/WARTS antibody (AB70561)
  • WB

Supplier Data

Western blot - Anti-LATS1/WARTS antibody (AB70561)

All lanes:

Western blot - Anti-LATS1/WARTS antibody (ab70561) at 0.2 µg/mL

Lane 1:

CT26.WT whole cell lysate at 50 µg

Lane 2:

TCMK-1 whole cell lysate at 50 µg

Predicted band size: 126 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human LATS1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

O95835

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/15000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/15000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab70561 was affinity purified using an epitope specific to LATS1/WARTS immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LATS1/WARTS also known as Large Tumor Suppressor 1 is a serine/threonine protein kinase with a molecular mass of approximately 125 kDa. Its expression is found in various tissue types including skin lung and liver. Mechanically LATS1/WARTS plays a critical role in regulating the cell cycle and maintaining genomic stability. This kinase is an essential part of the Hippo signaling pathway and contributes to controlling the size of organs by inhibiting cell proliferation and promoting apoptosis.
Biological function summary

LATS1/WARTS functions as an important regulator of cellular growth and differentiation. It is part of the Hippo signaling complex which includes proteins like MST1/2 and YAP/TAZ. This complex helps maintain proper tissue homeostasis by modulating gene expression in response to cellular density and mechanical cues. In doing so LATS1/WARTS phosphorylates and inactivates downstream effectors YAP and TAZ preventing them from translocating to the nucleus where they could promote transcription of growth-promoting genes.

Pathways

Scientists have found LATS1/WARTS to be integral to both the Hippo signaling pathway and the cell cycle regulation pathway. Within these pathways LATS1/WARTS interacts closely with proteins such as MOB1 and YAP mediating cellular responses to growth inhibitory signals. By controlling these interactions LATS1/WARTS helps coordinate cell proliferation and apoptosis which is critical for proper developmental processes and prevention of tumor growth.

Dysregulation of LATS1/WARTS function is associated with the development of cancers particularly in the liver and breast. Research also links LATS1/WARTS to neurodegenerative conditions by implicating its role in apoptosis and cell survival. When its kinase activity is compromised the regulatory balance maintained by the Hippo pathway is disrupted potentially leading to unchecked cell proliferation. Connections with proteins such as YAP and TAZ further illustrate its involvement in these pathological conditions showing its importance in maintaining cellular integrity and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed : 28068668).
See full target information LATS1
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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

General physiology and biophysics 44:259-274 PubMed40567070

2025

Knockdown of PLK1 suppresses malignant phenotypes and tumor growth in bladder cancer via activating Hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Yang,Yunhan Wang,Zhou Zheng,Hanchao Zhang,Haifeng Hu

Nature communications 15:9950 PubMed39550386

2024

GPRC5A promotes lung colonization of esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyu Zhou,Licheng Tan,Baifeng Zhang,Dora Lai Wan Kwong,Ching Ngar Wong,Yu Zhang,Beibei Ru,Yingchen Lyu,Kin To Hugo Siu,Jie Luo,Yuma Yang,Qin Liu,Yixin Chen,Weiguang Zhang,Chaohui He,Peng Jiang,Yanru Qin,Beilei Liu,Xin-Yuan Guan

Translational oncology 49:102076 PubMed39222611

2024

Effect of tumor-derived extracellular vesicle-shuttled lncRNA MALAT1 on proliferation, invasion and metastasis of triple-negative breast cancer by regulating macrophage M2 polarization via the POSTN/Hippo/YAP axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xuedong Wang,Qiwei Jian,Ziyun Zhang,Juan Gu,Xinping Wang,Yueping Wang

The Journal of biological chemistry 300:107212 PubMed38522513

2024

The tumor suppressor NF2 modulates TEAD4 stability and activity in Hippo signaling via direct interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Mengying Wu,Liqiao Hu,Lingli He,Liang Yuan,Lingling Yang,Bin Zhao,Lei Zhang,Xiaojing He

Stem cell reviews and reports 20:554-567 PubMed38150082

2023

miR-155-5p/Bmal1 Modulates the Senescence and Osteogenic Differentiation of Mouse BMSCs through the Hippo Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lanxin Zhang,Chengxiaoxue Zhang,Jiawen Zheng,Yuhong Wang,Xiaoyu Wei,Yuqing Yang,Qing Zhao

International journal of general medicine 16:467-480 PubMed36760683

2023

FNBP4 is a Potential Biomarker Associated with Cuproptosis and Promotes Tumor Progression in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Wen Zheng,Chao-Hua Zhang,Wu Wu,Zhu Zhu,Jian-Ping Gong,Chun-Ming Li

Oxidative medicine and cellular longevity 2022:4374978 PubMed36035221

2022

TRIM65 Promotes Malignant Cell Behaviors in Triple-Negative Breast Cancer by Impairing the Stability of LATS1 Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Yongbin Lu,Yi Xiao,Jingru Yang,Hongxin Su,Xiaobin Zhang,Fei Su,Baohong Tian,Da Zhao,Xiaoling Ling,Tao Zhang

Oncology letters 23:149 PubMed35350589

2022

causes changes in the host's expression of cancer-associated miRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Wang,Ning Wang,Ying Hui Zhao,Gang Lu

Open medicine (Warsaw, Poland) 17:453-462 PubMed35350839

2022

LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zeyi Ling,Xiaoli Long,Ying Wu,Jie Li,Mingliang Feng

Frontiers in oncology 12:839603 PubMed35311132

2022

α-Hederin Inhibits the Proliferation of Hepatocellular Carcinoma Cells Hippo-Yes-Associated Protein Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tongqing Chen,Dongdong Sun,Qijuan Wang,Tingting Zhou,Jiani Tan,Changliang Xu,Haibo Cheng,Weixing Shen
View all publications
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