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AB314123

Anti-LATS1/WARTS (phospho S909) + LATS2 (phospho S872) antibody [EPR27262-6] - BSA and Azide free

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Rabbit Recombinant Monoclonal LATS1/WARTS phospho S909 antibody. Carrier free. Suitable for Dot, ICC/IF, Flow Cyt (Intra) and reacts with Synthetic peptide, Human samples.

View Alternative Names

WARTS, LATS1, Serine/threonine-protein kinase LATS1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-LATS1/WARTS (phospho S909) + LATS2 (phospho S872) antibody [EPR27262-6] - BSA and Azide free (AB314123)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LATS1/WARTS (phospho S909) + LATS2 (phospho S872) antibody [EPR27262-6] - BSA and Azide free (AB314123)

This data was developed using ab314122, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling LATS1/WARTS (phospho S909) + LATS2 (phospho S872) with ab314122 at 1/100 (5.12 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and midbody staining in HeLa cells in M phase. Signal decreases after alkaline phosphatase treatment at 37℃ for 2h. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8)ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

Flow Cytometry (Intracellular) - Anti-LATS1/WARTS (phospho S909) + LATS2 (phospho S872) antibody [EPR27262-6] - BSA and Azide free (AB314123)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-LATS1/WARTS (phospho S909) + LATS2 (phospho S872) antibody [EPR27262-6] - BSA and Azide free (AB314123)

This data was developed using ab314122, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa treated with phosphatase at 37 ℃ overnight (human cervical adenocarcinoma epithelial cell) (Left) / Untreated HeLa (Right) cells labelling LATS1/WARTS (phospho S909) + LATS2 (phospho S872) with ab314122 at 1/50 dilution (1 ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. The bottom data shows total level of LATS1 is not affected by phosphatase treatment at 37 ℃ overnight.

Dot Blot - Anti-LATS1/WARTS (phospho S909) + LATS2 (phospho S872) antibody [EPR27262-6] - BSA and Azide free (AB314123)
  • Dot

Supplier Data

Dot Blot - Anti-LATS1/WARTS (phospho S909) + LATS2 (phospho S872) antibody [EPR27262-6] - BSA and Azide free (AB314123)

This data was developed using ab314122, the same antibody clone in a different buffer formulation. Dot blot analysis of LATS1/WARTS (phospho S909) + LATS2 (phospho S872) using ab314122 at 1 : 1000 (0.512 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST

  • Unconjugated

    Anti-LATS1/WARTS (phospho S909) + LATS2 (phospho S872) antibody [EPR27262-6]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27262-6

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab314123 is the carrier-free version of ab314122.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LATS1/LATS2 proteins also called WARTS are serine-threonine kinases involved in the Hippo signaling pathway with molecular masses of about 122 kDa and 104 kDa respectively. LATS1 and LATS2 are expressed in various tissues including the heart lung and liver. They work by phosphorylating other proteins which regulates cell proliferation and apoptosis. LATS proteins are found in the cytoplasm and can move into the nucleus influencing processes important for maintaining cellular homeostasis.
Biological function summary

LATS1 and LATS2 are part of the core Hippo signaling complex playing roles in controlling cell growth and organ size. They achieve this by inhibiting the transcription co-activator YAP/TAZ preventing excessive cell division and promoting programmed cell death when needed. This regulation ensures proper development and suppresses tumor formation. LATS proteins act as tumor suppressors preventing the overgrowth of cells that could lead to cancerous transformations.

Pathways

LATS1 and LATS2 integrate into the Hippo signaling and apoptotic pathways. In the Hippo pathway these kinases partner with MOB1 to phosphorylate YAP/TAZ preventing their translocation into the nucleus where they might trigger oncogenic gene expression. They also interact with other proteins like MST1/2 kinases within the Hippo cascade ensuring tight regulation of cell proliferation and death.

LATS1 and LATS2 are linked to cancer development and liver diseases. Dysregulation or mutations in these proteins can lead to unchecked cell growth posing a risk for cancers particularly liver cancer. Research shows that their interaction with other oncogenes like KRAS or tumor suppressors like p53 further influences their role in carcinogenesis. Additionally alterations in the Hippo pathway involving LATS proteins contribute to liver conditions potentially leading to hepatic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed : 28068668).
See full target information LATS1 phospho S909

Additional targets

LATS2 phospho S872
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Product promise

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