AB305030

Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal LATS1/WARTS phospho T1079 antibody. Carrier free. Suitable for Dot, WB, ICC/IF and reacts with Synthetic peptide, Human samples. Cited in 1 publication.

View Alternative Names

WARTS, LATS1, Serine/threonine-protein kinase LATS1, Large tumor suppressor homolog 1, WARTS protein kinase, h-warts, KPM, LATS2, Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Warts-like kinase

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

This data was developed using ab305029, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling LATS1(phospho T1079)+LATS2 (phospho T1041) with ab305029 at 1/100 (5.0 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing mainly centrosome staining in HeLa cells treated with Calyculin A (100 ng/ml) for 10 min. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 2 µg/ml dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

This data was developed using ab305029, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling LATS1(phospho T1079)+LATS2 (phospho T1041) with ab305029 at 1/100 (5.0 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing centriole (arrow) staining in HeLa cells in M phase. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5µg/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preabsorbed at 1/1000 2 µg/ml dilution.

Supplier Data

Western blot - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

This data was developed using ab305029, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST The antibody detects human LATS1 (phospho T1079) & LATS2 (phospho T1041). In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : Lanes 1-2 : 41 seconds, lanes 3-4 and 7-8 : 180 seconds, lanes 5-6 : 10 seconds.

All lanes:

Western blot - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] (<a href='/en-us/products/primary-antibodies/lats1-warts-phospho-t1079-lats2-phospho-t1041-antibody-epr27261-8-ab305029'>ab305029</a>) at 1/1000 dilution

Lanes 1 - 2:

HEK293T cells transfected with a human LATS1(S909A mutation) expression vector containing a his tag

Lanes 3 - 4:

HEK293T cells transfected with a human LATS1(T1079A mutation) expression vector containing a his tag

Lanes 5 - 6:

HEK293T cells transfected with a human LATS2(S872A mutation) expression vector containing a his tag

Lanes 7 - 8:

HEK293T cells transfected with a human LATS2(T1041A mutation) expression vector containing a his tag

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 150 kDa

false

Supplier Data

Western blot - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

This data was developed using , the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used immediately to minimize protein degradation. In Western blot, anti-LATS1 antibody (ab243656) staining at 1/1000 dilution. Anti-LATS2 antibody (ab243657) staining at 1/1000 dilution. Anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 15 seconds

All lanes:

Lane 1:

Untreated HeLa(human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg (untreated membrane) at 20 µg

Lane 2:

HeLa treated with 100 ng/mL Calyculin A for 10 minutes (untreated membrane)

Lane 3:

Untreated HeLa whole cell lysate 20 μg (phosphatase treated membrane) at 20 µg

Lane 4:

HeLa treated with 100 ng/mL Calyculin A for 10 minutes (phosphatase treated membrane)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa

false

Exposure time: 15s

Supplier Data

Dot Blot - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

This data was developed using ab305029, the same antibody clone in a different buffer formulation.

Dot blot analysis of LATS1(phospho T1079)+LATS2 (phospho T1041 using ab305029 at 1 : 1000 (0.5 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : LATS1 (phospho T1079)/ LATS2 (phospho T1041) peptide a

Lane 2 : LATS1 (phospho T1079)/ LATS2 (phospho T1041) peptide b

Lane 3 : LATS1/ LATS2 non-phospho peptide a

Peptide a, b and c share 100% sequence similarity with human LATS1 and LATS2.

Exposure time : 180 seconds

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Unconjugated

Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27261-8

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Dot, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Synthetic peptide": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The LATS1/LATS2 proteins also called WARTS are serine-threonine kinases involved in the Hippo signaling pathway with molecular masses of about 122 kDa and 104 kDa respectively. LATS1 and LATS2 are expressed in various tissues including the heart lung and liver. They work by phosphorylating other proteins which regulates cell proliferation and apoptosis. LATS proteins are found in the cytoplasm and can move into the nucleus influencing processes important for maintaining cellular homeostasis.

Biological function summary

LATS1 and LATS2 are part of the core Hippo signaling complex playing roles in controlling cell growth and organ size. They achieve this by inhibiting the transcription co-activator YAP/TAZ preventing excessive cell division and promoting programmed cell death when needed. This regulation ensures proper development and suppresses tumor formation. LATS proteins act as tumor suppressors preventing the overgrowth of cells that could lead to cancerous transformations.

Pathways

LATS1 and LATS2 integrate into the Hippo signaling and apoptotic pathways. In the Hippo pathway these kinases partner with MOB1 to phosphorylate YAP/TAZ preventing their translocation into the nucleus where they might trigger oncogenic gene expression. They also interact with other proteins like MST1/2 kinases within the Hippo cascade ensuring tight regulation of cell proliferation and death.

LATS1 and LATS2 are linked to cancer development and liver diseases. Dysregulation or mutations in these proteins can lead to unchecked cell growth posing a risk for cancers particularly liver cancer. Research shows that their interaction with other oncogenes like KRAS or tumor suppressors like p53 further influences their role in carcinogenesis. Additionally alterations in the Hippo pathway involving LATS proteins contribute to liver conditions potentially leading to hepatic disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed : 10518011, PubMed : 10831611, PubMed : 18158288, PubMed : 26437443, PubMed : 28068668). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed : 18158288, PubMed : 26437443, PubMed : 28068668). Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed : 18158288, PubMed : 26437443, PubMed : 28068668). Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint (PubMed : 15122335, PubMed : 19927127). Negatively regulates G2/M transition by down-regulating CDK1 kinase activity (PubMed : 9988268). Involved in the control of p53 expression (PubMed : 15122335). Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1 (PubMed : 15220930). May also play a role in endocrine function. Plays a role in mammary gland epithelial cell differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed : 28068668). Acts as an activator of the NLRP3 inflammasome by mediating phosphorylation of 'Ser-265' of NLRP3 following NLRP3 palmitoylation, promoting NLRP3 activation by NEK7 (PubMed : 39173637).

See full target information LATS1 pT1079

Additional targets

LATS2 phospho T1041

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 239:e31220 PubMed38372068

2024

mA-induced TRIB3 regulates Hippo pathway through interacting with LATS1 to promote the progression of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiamei Wu,Tingzhuang Yi,Chenyi Zhuo,Duanduan Wang,Ming Zhang,Rui Hu,Dan Wu,Guoxin Hou,Yutong Xing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

Immunocytochemistry/ Immunofluorescence - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

Western blot - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

Western blot - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

Dot Blot - Anti-LATS1/WARTS (phospho T1079) + LATS2 (phospho T1041) antibody [EPR27261-8] - BSA and Azide free (AB305030)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise