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AB243657

Anti-LATS2 antibody [EPR23126-488]

  • 20ul selling size
  • KO Validated
  • RabMAb
  • Recombinant
  • What is this?

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(7 Publications)

Anti-LATS2 antibody [EPR23126-488] (ab243657) is a rabbit monoclonal antibody detecting LATS2 in Western Blot, IP. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

KPM, LATS2, Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Warts-like kinase

5 Images
Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] (AB243657)
  • IP

Supplier Data

Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] (AB243657)

LATS2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab243657 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243657 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

Lane 2 : ab243657 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab243657 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 64 seconds

Fresh lysates were used in this WB.

All lanes:

Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] (ab243657)

Predicted band size: 120 kDa

Observed band size: 140 kDa

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Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657)
  • WB

Lab

Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Fresh lysates were used in this WB.

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 3 minutes

All lanes:

Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting LATS2, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 120 kDa

Observed band size: 140 kDa

false

Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657)
  • WB

Lab

Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Fresh lysates were used in this WB.

Exposure time : 3 minutes

All lanes:

Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 120 kDa

Observed band size: 140 kDa

false

Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657)
  • WB

Lab

Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657)

Western blot : Anti-LATS2 antibody [EPR23126-488] (ab243657) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab243657 was shown to bind specifically to LATS2. A band was observed at 117-171 kDa in wild-type A549 cell lysates with no signal observed at this size in LATS2 knockout cell line. To generate this image, wild-type and LATS2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human LATS2 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-lats2-knockout-a549-cell-line-ab286746'>ab286746</a>)

Lane 2:

LATS2 knockout A549 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 117-171 kDa

false

Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657)
  • WB

Lab

Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

Lane 1:

His-tagged human LATS1 recombinant protein (aa701-1054) at 0.01 µg

Lane 2:

His-tagged human LATS2 recombinant protein (aa664-1016) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 120 kDa

false

  • Carrier free

    Anti-LATS2 antibody [EPR23126-488] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23126-488

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Anti-YAP1 (phospho S127) antibody [EP1675Y]

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Secondary Antibodies

AB150077

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  • 5
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Primary Antibodies

AB52641

Anti-STK3/MST-2 antibody [EP1466Y]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3/MST-2 antibody [EP1466Y] (AB52641)

  • 0
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Reactivity data

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Product details

What is this antibody validated in?
Anti-LATS2 antibody [EPR23126-488] (ab243657) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Human samples.

What is the molecular weight of LATS2?
Anti-LATS2 [EPR23126-488] (ab243657) specifically detects a band for LATS2 (UniProt: Q9NRM7) at a molecular weight of 120kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-LATS2 antibody [EPR23126-488] (ab243657) has been confirmed by Western blot testing in LATS2 Knockout A549 cell line.

Other related products
We have a range of other formats of antibody clone [EPR23126-488] also available for your convenience: ab243657, Carrier free - ab280363

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LATS2 also known as Large Tumor Suppressor kinase 2 is a serine/threonine-protein kinase with a molecular mass of approximately 121 kDa. It functions as a component of the Hippo signaling pathway where it helps regulate cell proliferation apoptosis and organ size. LATS2 shows expression in various tissues with notable levels in the lung heart and liver. Its role in these tissues makes it an important target for research in cancer and developmental biology.
Biological function summary

LATS2 plays an important role in cell cycle regulation and tumor suppression. LATS2 forms part of the core kinase complex within the Hippo pathway working closely with other proteins like MST1/2 kinases and Scaffold proteins SAV1. Through phosphorylation LATS2 can inhibit the activity of YAP and TAZ transcription co-activators promoting cell contact inhibition and proper tissue architecture. Its activity is important for limiting excessive cell growth and ensuring accurate cell division.

Pathways

LATS2 integrates into the Hippo signaling pathway and influences the PI3K-AKT signaling pathway. Within the Hippo pathway LATS2 interacts with MST1/2 forming an upstream kinase complex activating through phosphorylation of downstream transcription co-activators YAP and TAZ. These interactions result in the transcriptional regulation of genes controlling cell growth and apoptosis. Overlap with the PI3K-AKT pathway links LATS2 to cellular processes related to growth migration and survival providing a critical nexus that communicates cell status and environmental cues.

LATS2 has associations with both cancer and cardiac hypertrophy. In cancer dysregulation of LATS2 activity can result in aberrant cell growth and tumor development often noted in different types of carcinomas where it can act as a tumor suppressor. Mutations or reduced expression of LATS2 can correlate with increased cancer progression implying a need for comprehensive understanding of its regulation and function. Additionally in cardiac hypertrophy LATS2 contributes to cardiac cell size regulation and its imbalance can connect to abnormal heart muscle thickening. In these contexts LATS2 interaction with proteins such as YAP and TAZ provides an important point of interest for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed : 18158288, PubMed : 26437443, PubMed : 26598551, PubMed : 34404733). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed : 26437443, PubMed : 26598551, PubMed : 34404733). Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed : 26598551, PubMed : 34404733). Also phosphorylates YAP1 in response to cell contact inhibition-driven WWP1 ubiquitination of AMOTL2, which results in LATS2 activation (PubMed : 34404733). Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability (PubMed : 10871863). Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity (PubMed : 12853976). Negative regulator of the androgen receptor (PubMed : 15131260). Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities (PubMed : 21952048). This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ (PubMed : 21952048). Acts as an activator of the NLRP3 inflammasome by mediating phosphorylation of 'Ser-265' of NLRP3 following NLRP3 palmitoylation, promoting NLRP3 activation by NEK7 (PubMed : 39173637).
See full target information LATS2
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:1047-1056 PubMed40151143

2025

Breast cancer-derived exosomal miR-105-5p facilitates the transformation of NFs into CAFs through LATS2-NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodi Ding,Zhimei Sheng,Jiayu Cui,Meimei Cui,Liying Zhang,Ruijun Feng,Yongming Wang,Wei Sun,Xiurong Zhang,Lihong Shi,Baogang Zhang

iScience 27:109667 PubMed38966570

2024

upregulated by promotes ferroptosis via inhibiting axis in sepsis-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Lv,Jing Yu,Xingjia Qian,Jun Shu,Qiuhong Qian,Luhong Shen,Dongfang Shi,Zhengzheng Tao,Guiqin Fan,Bufeng Zhuang,Bing Lu

The Journal of biological chemistry 300:107212 PubMed38522513

2024

The tumor suppressor NF2 modulates TEAD4 stability and activity in Hippo signaling via direct interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Mengying Wu,Liqiao Hu,Lingli He,Liang Yuan,Lingling Yang,Bin Zhao,Lei Zhang,Xiaojing He

Discover oncology 15:73 PubMed38478152

2024

GALNT2 targeted by miR-139-5p promotes proliferation of clear cell renal cell carcinoma via inhibition of LATS2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Haisheng Yi,Lingyun Liu,Jingshun Zhang,Kaimin Guo,Yin Cao,Penghao Sun,Hongliang Wang

Neoplasma 69:1079-1091 PubMed35818964

2022

hsa_circ_0067514 suppresses gastric cancer progression and glycolysis via miR-654-3p/LATS2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Feng Jiang,Rong-Rong Li

Cell death and differentiation 29:206-217 PubMed34465890

2021

Mechanosensitive turnover of phosphoribosyl pyrophosphate synthetases regulates nucleotide metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Jichun Shao,Zhijun Zeng,Yumin He,Can Tang,Su Hwan Park,Jong-Ho Lee,Rui Liu

Oncogene 39:7181-7195 PubMed33037411

2020

S6K1 blockade overcomes acquired resistance to EGFR-TKIs in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Shen,Gao-Chan Wang,Xiang Li,Xin Ge,Meng Wang,Zhu-Mei Shi,Vikas Bhardwaj,Zi-Xuan Wang,Ralph G Zinner,Stephen C Peiper,Andrew E Aplin,Bing-Hua Jiang,Jun He
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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