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Rabbit Recombinant Monoclonal LATS2 antibody. Suitable for IP, WB and reacts with Human, Transfected cell lysate samples. Cited in 2 publications.

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Images

Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] (AB243657), expandable thumbnail
  • Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657), expandable thumbnail
  • Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657), expandable thumbnail
  • Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657), expandable thumbnail
  • Western blot - Anti-LATS2 antibody [EPR23126-488] (AB243657), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-P
Human
Not recommended
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell lysate, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Transfected cell lysate, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell lysate
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate
Dilution info
-
Notes

-

Target data

Function

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:26598551, PubMed:34404733). Also phosphorylates YAP1 in response to cell contact inhibition-driven WWP1 ubiquitination of AMOTL2, which results in LATS2 activation (PubMed:34404733). Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.

Alternative names

KPM, LATS2, Serine/threonine-protein kinase LATS2, Kinase phosphorylated during mitosis protein, Large tumor suppressor homolog 2, Serine/threonine-protein kinase kpm, Warts-like kinase

Recommended products

Rabbit Recombinant Monoclonal LATS2 antibody. Suitable for IP, WB and reacts with Human, Transfected cell lysate samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23126-488
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LATS2 also known as Large Tumor Suppressor kinase 2 is a serine/threonine-protein kinase with a molecular mass of approximately 121 kDa. It functions as a component of the Hippo signaling pathway where it helps regulate cell proliferation apoptosis and organ size. LATS2 shows expression in various tissues with notable levels in the lung heart and liver. Its role in these tissues makes it an important target for research in cancer and developmental biology.

Biological function summary

LATS2 plays an important role in cell cycle regulation and tumor suppression. LATS2 forms part of the core kinase complex within the Hippo pathway working closely with other proteins like MST1/2 kinases and Scaffold proteins SAV1. Through phosphorylation LATS2 can inhibit the activity of YAP and TAZ transcription co-activators promoting cell contact inhibition and proper tissue architecture. Its activity is important for limiting excessive cell growth and ensuring accurate cell division.

Pathways

LATS2 integrates into the Hippo signaling pathway and influences the PI3K-AKT signaling pathway. Within the Hippo pathway LATS2 interacts with MST1/2 forming an upstream kinase complex activating through phosphorylation of downstream transcription co-activators YAP and TAZ. These interactions result in the transcriptional regulation of genes controlling cell growth and apoptosis. Overlap with the PI3K-AKT pathway links LATS2 to cellular processes related to growth migration and survival providing a critical nexus that communicates cell status and environmental cues.

Associated diseases and disorders

LATS2 has associations with both cancer and cardiac hypertrophy. In cancer dysregulation of LATS2 activity can result in aberrant cell growth and tumor development often noted in different types of carcinomas where it can act as a tumor suppressor. Mutations or reduced expression of LATS2 can correlate with increased cancer progression implying a need for comprehensive understanding of its regulation and function. Additionally in cardiac hypertrophy LATS2 contributes to cardiac cell size regulation and its imbalance can connect to abnormal heart muscle thickening. In these contexts LATS2 interaction with proteins such as YAP and TAZ provides an important point of interest for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] (ab243657), expandable thumbnail

    Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] (ab243657)

    LATS2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab243657 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243657 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab243657 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab243657 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 64 seconds

    Fresh lysates were used in this WB.

    All lanes: Immunoprecipitation - Anti-LATS2 antibody [EPR23126-488] (ab243657)

    Predicted band size: 120 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657), expandable thumbnail

    Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Fresh lysates were used in this WB.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

    Lane 2: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 120 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657), expandable thumbnail

    Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Fresh lysates were used in this WB.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), transfected with scrambled siRNA control, whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting LATS2, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 120 kDa

    Observed band size: 140 kDa

  • Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657), expandable thumbnail

    Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

    Lane 1: His-tagged human LATS1 recombinant protein (aa701-1054) at 0.01 µg

    Lane 2: His-tagged human LATS2 recombinant protein (aa664-1016) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 120 kDa

  • Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657), expandable thumbnail

    Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657)

    Western blot: Anti-LATS2 antibody [EPR23126-488] (ab243657) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab243657 was shown to bind specifically to LATS2. A band was observed at 117-171 kDa in wild-type A549 cell lysates with no signal observed at this size in LATS2 knockout cell line. To generate this image, wild-type and LATS2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-LATS2 antibody [EPR23126-488] (ab243657) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: Western blot - Human LATS2 knockout A549 cell line (Human LATS2 knockout A549 cell line ab286746)

    Lane 2: LATS2 knockout A549 cell lysate at 20 µg

    Lane 3: MCF7 cell lysate at 20 µg

    Lane 4: Raji cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

    Lanes 1 - 4: Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 117-171 kDa

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Product protocols

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